Affordable amino acid α/β-deuteration and specific labeling for NMR signal enhancement: Evaluation on the kinase p38α

Affordable amino acid α/β-deuteration and specific labeling for NMR signal enhancement: Evaluation on the kinase p38α

Although very effective in decreasing NMR relaxation of large proteins, homogeneous deuteration can be costly, and anyway unsuitable for recombinant production in metazoan systems. We sought to explore other deuteration schemes, which would be adapted to protein expression in mammalian cells. Here,...

Full description

Bibliographic Details
Main Authors: Rania Ghouil, Chafiaa Bouguechtouli, Hélène Chérot, Agathe Marcelot, Maxime Roche, Francois-Xavier Theillet
Format: Article
Language:English
Published: Elsevier 2023-12-01
Series:Journal of Magnetic Resonance Open
Subjects:
Kinase
p38
Cell-free expression
Deuteration
In-cell NMR
Online Access:http://www.sciencedirect.com/science/article/pii/S2666441023000341

Similar Items

Similar Items