HEK293 Cell Line as a Platform to Produce Recombinant Proteins and Viral Vectors
Animal cell-based expression platforms enable the production of complex biomolecules such as recombinant proteins and viral vectors. Although most biotherapeutics are produced in animal cell lines, production in human cell lines is expanding. One important advantage of using human cell lines is the...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-12-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2021.796991/full |
| _version_ | 1818987153995071488 |
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| author | Evan Tan Cara Sze Hui Chin Zhi Feng Sherman Lim Say Kong Ng |
| author_facet | Evan Tan Cara Sze Hui Chin Zhi Feng Sherman Lim Say Kong Ng |
| author_sort | Evan Tan |
| collection | DOAJ |
| description | Animal cell-based expression platforms enable the production of complex biomolecules such as recombinant proteins and viral vectors. Although most biotherapeutics are produced in animal cell lines, production in human cell lines is expanding. One important advantage of using human cell lines is the increased potential that the resulting biotherapeutics would carry more “human-like” post-translational modifications. Among the human cell lines, HEK293 is widely utilized due to its high transfectivity, rapid growth rate, and ability to grow in a serum-free, suspension culture. In this review, we discuss the use of HEK293 cells and its subtypes in the production of biotherapeutics. We also compare their usage against other commonly used host cell lines in each category of biotherapeutics and summarise the factors influencing the choice of host cell lines used. |
| first_indexed | 2024-12-20T19:02:10Z |
| format | Article |
| id | doaj.art-3039674df5144cb3b24da5f9ef91cc9f |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-20T19:02:10Z |
| publishDate | 2021-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-3039674df5144cb3b24da5f9ef91cc9f2022-12-21T19:29:21ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852021-12-01910.3389/fbioe.2021.796991796991HEK293 Cell Line as a Platform to Produce Recombinant Proteins and Viral VectorsEvan TanCara Sze Hui ChinZhi Feng Sherman LimSay Kong NgAnimal cell-based expression platforms enable the production of complex biomolecules such as recombinant proteins and viral vectors. Although most biotherapeutics are produced in animal cell lines, production in human cell lines is expanding. One important advantage of using human cell lines is the increased potential that the resulting biotherapeutics would carry more “human-like” post-translational modifications. Among the human cell lines, HEK293 is widely utilized due to its high transfectivity, rapid growth rate, and ability to grow in a serum-free, suspension culture. In this review, we discuss the use of HEK293 cells and its subtypes in the production of biotherapeutics. We also compare their usage against other commonly used host cell lines in each category of biotherapeutics and summarise the factors influencing the choice of host cell lines used.https://www.frontiersin.org/articles/10.3389/fbioe.2021.796991/fullHEK293human embryonic kidney cell 293biotechnologyviral vectorsviral vectored vaccinesbiotherapeutics and biologics |
| spellingShingle | Evan Tan Cara Sze Hui Chin Zhi Feng Sherman Lim Say Kong Ng HEK293 Cell Line as a Platform to Produce Recombinant Proteins and Viral Vectors Frontiers in Bioengineering and Biotechnology HEK293 human embryonic kidney cell 293 biotechnology viral vectors viral vectored vaccines biotherapeutics and biologics |
| title | HEK293 Cell Line as a Platform to Produce Recombinant Proteins and Viral Vectors |
| title_full | HEK293 Cell Line as a Platform to Produce Recombinant Proteins and Viral Vectors |
| title_fullStr | HEK293 Cell Line as a Platform to Produce Recombinant Proteins and Viral Vectors |
| title_full_unstemmed | HEK293 Cell Line as a Platform to Produce Recombinant Proteins and Viral Vectors |
| title_short | HEK293 Cell Line as a Platform to Produce Recombinant Proteins and Viral Vectors |
| title_sort | hek293 cell line as a platform to produce recombinant proteins and viral vectors |
| topic | HEK293 human embryonic kidney cell 293 biotechnology viral vectors viral vectored vaccines biotherapeutics and biologics |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2021.796991/full |
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