Brassavola tuberculata Hook.: in vitro growth and ex vitro establishment as a function of the micropropagation system and sucrose
Abstract This study examines the in vitro growth and ex vitro establishment of Brassavola tuberculata in relation to the micropropagation system and sucrose concentration employed in the in vitro culture. A completely randomized experimental design was utilized, employing a 2 x 5 factorial arrangeme...
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| Format: | Article |
| Language: | English |
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Instituto Internacional de Ecologia
2023-07-01
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| Series: | Brazilian Journal of Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842023000100813&lng=en&tlng=en |
| _version_ | 1797777846557474816 |
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| author | J. S. Soares J. C. M. Ramos J. C. Sorgato L. M. Ribeiro L. C. Reis |
| author_facet | J. S. Soares J. C. M. Ramos J. C. Sorgato L. M. Ribeiro L. C. Reis |
| author_sort | J. S. Soares |
| collection | DOAJ |
| description | Abstract This study examines the in vitro growth and ex vitro establishment of Brassavola tuberculata in relation to the micropropagation system and sucrose concentration employed in the in vitro culture. A completely randomized experimental design was utilized, employing a 2 x 5 factorial arrangement. The experimental period began with seedlings cultivated in vitro for 180 days, which were subsequently transferred to Murashige and Skoog culture media containing sucrose concentrations of 0, 15, 30, 45, or 60 g L-1. The cultures were subjected to two micropropagation systems: conventional and gas exchange. After 90 days of in vitro cultivation, the plants were evaluated, transplanted into a substrate, and placed in a screened nursery for ex vitro cultivation. After 300 days of ex vitro cultivation, the survival and initial characteristics of the plants were assessed. The micropropagation system allowing gas exchange and sucrose concentrations up to 30 g L-1 enhanced the shoot and root growth of in vitro propagated plants. No noticeable anatomical differences were observed after 90 days of in vitro culture among the different sucrose concentrations and micropropagation systems used. In the ex vitro establishment, irrespective of sucrose concentration, the micropropagation system facilitating gas exchange positively influenced all evaluated characteristics. |
| first_indexed | 2024-03-12T23:09:24Z |
| format | Article |
| id | doaj.art-303b3b9ca909476d9fe0a2ca3b944ee8 |
| institution | Directory Open Access Journal |
| issn | 1678-4375 |
| language | English |
| last_indexed | 2024-03-12T23:09:24Z |
| publishDate | 2023-07-01 |
| publisher | Instituto Internacional de Ecologia |
| record_format | Article |
| series | Brazilian Journal of Biology |
| spelling | doaj.art-303b3b9ca909476d9fe0a2ca3b944ee82023-07-18T07:48:42ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-43752023-07-018310.1590/1519-6984.270892Brassavola tuberculata Hook.: in vitro growth and ex vitro establishment as a function of the micropropagation system and sucroseJ. S. Soareshttps://orcid.org/0000-0001-9497-2975J. C. M. Ramoshttps://orcid.org/0000-0002-3987-6726J. C. Sorgatohttps://orcid.org/0000-0003-0001-5467L. M. Ribeirohttps://orcid.org/0000-0002-7278-7696L. C. Reishttps://orcid.org/0000-0002-2494-209XAbstract This study examines the in vitro growth and ex vitro establishment of Brassavola tuberculata in relation to the micropropagation system and sucrose concentration employed in the in vitro culture. A completely randomized experimental design was utilized, employing a 2 x 5 factorial arrangement. The experimental period began with seedlings cultivated in vitro for 180 days, which were subsequently transferred to Murashige and Skoog culture media containing sucrose concentrations of 0, 15, 30, 45, or 60 g L-1. The cultures were subjected to two micropropagation systems: conventional and gas exchange. After 90 days of in vitro cultivation, the plants were evaluated, transplanted into a substrate, and placed in a screened nursery for ex vitro cultivation. After 300 days of ex vitro cultivation, the survival and initial characteristics of the plants were assessed. The micropropagation system allowing gas exchange and sucrose concentrations up to 30 g L-1 enhanced the shoot and root growth of in vitro propagated plants. No noticeable anatomical differences were observed after 90 days of in vitro culture among the different sucrose concentrations and micropropagation systems used. In the ex vitro establishment, irrespective of sucrose concentration, the micropropagation system facilitating gas exchange positively influenced all evaluated characteristics.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842023000100813&lng=en&tlng=enin vitro cultivationphotoautotrophicphotomixotrophicacclimatizationornamental horticulturenative species |
| spellingShingle | J. S. Soares J. C. M. Ramos J. C. Sorgato L. M. Ribeiro L. C. Reis Brassavola tuberculata Hook.: in vitro growth and ex vitro establishment as a function of the micropropagation system and sucrose Brazilian Journal of Biology in vitro cultivation photoautotrophic photomixotrophic acclimatization ornamental horticulture native species |
| title | Brassavola tuberculata Hook.: in vitro growth and ex vitro establishment as a function of the micropropagation system and sucrose |
| title_full | Brassavola tuberculata Hook.: in vitro growth and ex vitro establishment as a function of the micropropagation system and sucrose |
| title_fullStr | Brassavola tuberculata Hook.: in vitro growth and ex vitro establishment as a function of the micropropagation system and sucrose |
| title_full_unstemmed | Brassavola tuberculata Hook.: in vitro growth and ex vitro establishment as a function of the micropropagation system and sucrose |
| title_short | Brassavola tuberculata Hook.: in vitro growth and ex vitro establishment as a function of the micropropagation system and sucrose |
| title_sort | brassavola tuberculata hook in vitro growth and ex vitro establishment as a function of the micropropagation system and sucrose |
| topic | in vitro cultivation photoautotrophic photomixotrophic acclimatization ornamental horticulture native species |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842023000100813&lng=en&tlng=en |
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