Ethanol and supercritical fluid extracts of hemp seed (Cannabis sativa L.) increase gene expression of antioxidant enzymes in HepG2 cells
Objective: To determine the gene expression of antioxidant enzymes by hemp seed extracts in human hepatoma (HepG2) cells. Methods: Ethanol and supercritical fluid (SF) extracts obtained from de-hulled hemp seed were used for the evaluation of in vitro antioxidant activity and gene expression of anti...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2015-06-01
|
| Series: | Asian Pacific Journal of Reproduction |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2305050015300129 |
| _version_ | 1818062350101512192 |
|---|---|
| author | Sunghyun Hong Kandhasamy Sowndhararajan Taewoo Joo Chanmook Lim Haeme Cho Songmun Kim Gur-Yoo Kim Jin-Woo Jhoo |
| author_facet | Sunghyun Hong Kandhasamy Sowndhararajan Taewoo Joo Chanmook Lim Haeme Cho Songmun Kim Gur-Yoo Kim Jin-Woo Jhoo |
| author_sort | Sunghyun Hong |
| collection | DOAJ |
| description | Objective: To determine the gene expression of antioxidant enzymes by hemp seed extracts in human hepatoma (HepG2) cells.
Methods: Ethanol and supercritical fluid (SF) extracts obtained from de-hulled hemp seed were used for the evaluation of in vitro antioxidant activity and gene expression of antioxidant enzymes. In vitro antioxidant activities of the samples evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) radical scavenging assays. The expression of antioxidant enzymes such as superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) in HepG2 cells was evaluated by real-time PCR.
Results: In the antioxidant assay, SF extract of hemp seed exhibited higher ABTS and DPPH radical scavenging activities (IC50 of 66.6 µg/mL and 9.2 mg/mL, respectively) than ethanol extract. The results of antioxidant enzyme expression in real-time PCR study revealed the H2O2 (200 µM) challenged HepG2 cells reduced the expression of enzymes such as SOD, GPx and CAT. However, the cells treated with ethanol and SF extracts were up-regulated the expression of antioxidant enzymes in concentration dependent manner. When compared to ethanol extract, the SF extract exhibited higher activity in the expression of all the antioxidant enzymes at the concentration of 500 µg/mL.
Conclusions: In conclusion, the findings of our study demonstrated that the hemp seed effectively inhibited H2O2 mediated oxidative stress and may be useful as a therapeutic agent in preventing oxidative stress mediated diseases. |
| first_indexed | 2024-12-10T14:02:48Z |
| format | Article |
| id | doaj.art-303b59cd611849dbb37f46888856d8d6 |
| institution | Directory Open Access Journal |
| issn | 2305-0500 |
| language | English |
| last_indexed | 2024-12-10T14:02:48Z |
| publishDate | 2015-06-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Reproduction |
| spelling | doaj.art-303b59cd611849dbb37f46888856d8d62022-12-22T01:45:45ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Reproduction2305-05002015-06-014214715210.1016/S2305-0500(15)30012-9Ethanol and supercritical fluid extracts of hemp seed (Cannabis sativa L.) increase gene expression of antioxidant enzymes in HepG2 cellsSunghyun Hong0Kandhasamy Sowndhararajan1Taewoo Joo2Chanmook Lim3Haeme Cho4Songmun Kim5Gur-Yoo Kim6Jin-Woo Jhoo7Kangwon National University, Department of Animal Products and Food Science, Chuncheon, Gangwon, Republic of Korea, 200-701Department of Process and Food Engineering, Faculty of Engineering, Universiti Putra Malaysia, Serdang, Selangor, Malaysia, 43400Kangwon National University, Department of Animal Products and Food Science, Chuncheon, Gangwon, Republic of Korea, 200-701Kangwon National University, Department of Animal Products and Food Science, Chuncheon, Gangwon, Republic of Korea, 200-701Kangwon National University, Department of Biological Environment, Chuncheon, Gangwon, Republic of Korea, 200-701Kangwon National University, Department of Biological Environment, Chuncheon, Gangwon, Republic of Korea, 200-701Kangwon National University, Department of Animal Products and Food Science, Chuncheon, Gangwon, Republic of Korea, 200-701Kangwon National University, Department of Animal Products and Food Science, Chuncheon, Gangwon, Republic of Korea, 200-701Objective: To determine the gene expression of antioxidant enzymes by hemp seed extracts in human hepatoma (HepG2) cells. Methods: Ethanol and supercritical fluid (SF) extracts obtained from de-hulled hemp seed were used for the evaluation of in vitro antioxidant activity and gene expression of antioxidant enzymes. In vitro antioxidant activities of the samples evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) radical scavenging assays. The expression of antioxidant enzymes such as superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) in HepG2 cells was evaluated by real-time PCR. Results: In the antioxidant assay, SF extract of hemp seed exhibited higher ABTS and DPPH radical scavenging activities (IC50 of 66.6 µg/mL and 9.2 mg/mL, respectively) than ethanol extract. The results of antioxidant enzyme expression in real-time PCR study revealed the H2O2 (200 µM) challenged HepG2 cells reduced the expression of enzymes such as SOD, GPx and CAT. However, the cells treated with ethanol and SF extracts were up-regulated the expression of antioxidant enzymes in concentration dependent manner. When compared to ethanol extract, the SF extract exhibited higher activity in the expression of all the antioxidant enzymes at the concentration of 500 µg/mL. Conclusions: In conclusion, the findings of our study demonstrated that the hemp seed effectively inhibited H2O2 mediated oxidative stress and may be useful as a therapeutic agent in preventing oxidative stress mediated diseases.http://www.sciencedirect.com/science/article/pii/S2305050015300129Antioxidant enzymeCannabis sativaHemp seedHepG2 cellsCatalaseSuperoxide dismutase |
| spellingShingle | Sunghyun Hong Kandhasamy Sowndhararajan Taewoo Joo Chanmook Lim Haeme Cho Songmun Kim Gur-Yoo Kim Jin-Woo Jhoo Ethanol and supercritical fluid extracts of hemp seed (Cannabis sativa L.) increase gene expression of antioxidant enzymes in HepG2 cells Asian Pacific Journal of Reproduction Antioxidant enzyme Cannabis sativa Hemp seed HepG2 cells Catalase Superoxide dismutase |
| title | Ethanol and supercritical fluid extracts of hemp seed (Cannabis sativa L.) increase gene expression of antioxidant enzymes in HepG2 cells |
| title_full | Ethanol and supercritical fluid extracts of hemp seed (Cannabis sativa L.) increase gene expression of antioxidant enzymes in HepG2 cells |
| title_fullStr | Ethanol and supercritical fluid extracts of hemp seed (Cannabis sativa L.) increase gene expression of antioxidant enzymes in HepG2 cells |
| title_full_unstemmed | Ethanol and supercritical fluid extracts of hemp seed (Cannabis sativa L.) increase gene expression of antioxidant enzymes in HepG2 cells |
| title_short | Ethanol and supercritical fluid extracts of hemp seed (Cannabis sativa L.) increase gene expression of antioxidant enzymes in HepG2 cells |
| title_sort | ethanol and supercritical fluid extracts of hemp seed cannabis sativa l increase gene expression of antioxidant enzymes in hepg2 cells |
| topic | Antioxidant enzyme Cannabis sativa Hemp seed HepG2 cells Catalase Superoxide dismutase |
| url | http://www.sciencedirect.com/science/article/pii/S2305050015300129 |
| work_keys_str_mv | AT sunghyunhong ethanolandsupercriticalfluidextractsofhempseedcannabissativalincreasegeneexpressionofantioxidantenzymesinhepg2cells AT kandhasamysowndhararajan ethanolandsupercriticalfluidextractsofhempseedcannabissativalincreasegeneexpressionofantioxidantenzymesinhepg2cells AT taewoojoo ethanolandsupercriticalfluidextractsofhempseedcannabissativalincreasegeneexpressionofantioxidantenzymesinhepg2cells AT chanmooklim ethanolandsupercriticalfluidextractsofhempseedcannabissativalincreasegeneexpressionofantioxidantenzymesinhepg2cells AT haemecho ethanolandsupercriticalfluidextractsofhempseedcannabissativalincreasegeneexpressionofantioxidantenzymesinhepg2cells AT songmunkim ethanolandsupercriticalfluidextractsofhempseedcannabissativalincreasegeneexpressionofantioxidantenzymesinhepg2cells AT guryookim ethanolandsupercriticalfluidextractsofhempseedcannabissativalincreasegeneexpressionofantioxidantenzymesinhepg2cells AT jinwoojhoo ethanolandsupercriticalfluidextractsofhempseedcannabissativalincreasegeneexpressionofantioxidantenzymesinhepg2cells |