DLP fabrication of HA scaffold with customized porous structures to regulate immune microenvironment and macrophage polarization for enhancing bone regeneration
The immune microenvironment plays a pivotal role in osteoanagenesis. Biomaterials can modulate osteogenic efficacy by inducing specific local immune reactions. As 3D-printing technology advances, digital light projection printing has emerged as a promising method for creating large scale, high-preci...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-02-01
|
| Series: | Materials Today Bio |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006423003897 |
| _version_ | 1827381682285576192 |
|---|---|
| author | Shilang Xiong Yinuo Zhang Jianhua Zeng Jingyu Zhou Shiwei Liu Peng Wei Hantian Liu Feng Yi Zongmiao Wan Long Xiong Bin Zhang Jingtang Li |
| author_facet | Shilang Xiong Yinuo Zhang Jianhua Zeng Jingyu Zhou Shiwei Liu Peng Wei Hantian Liu Feng Yi Zongmiao Wan Long Xiong Bin Zhang Jingtang Li |
| author_sort | Shilang Xiong |
| collection | DOAJ |
| description | The immune microenvironment plays a pivotal role in osteoanagenesis. Biomaterials can modulate osteogenic efficacy by inducing specific local immune reactions. As 3D-printing technology advances, digital light projection printing has emerged as a promising method for creating large scale, high-precision biomaterial scaffolds. By adjusting the solid content and the sintering conditions during printing, the pore size of biomaterials can be meticulously controlled. Yet, the systematic influence of pore size on the immune microenvironment remains uncharted. We fabricated 3D-printed hydroxyapatite bioceramic scaffolds with three distinct pore sizes: 400 μm, 600 μm, and 800 μm. Our study revealed that scaffolds with a pore size of 600 μm promote macrophage M2 polarization, which is achieved by upregulating interferon−beta and HIF-1α production. When these materials were implanted subcutaneously in rats and within rabbit skulls, we observed that the 600 μm scaffolds notably improved the long-term inflammatory response, fostered vascular proliferation, and augmented new bone growth. This research paves the way for innovative therapeutic strategies for treating large segmental bone defects in clinical settings. |
| first_indexed | 2024-03-08T14:07:49Z |
| format | Article |
| id | doaj.art-303dd0863eb34f63a40a800b264b8ef1 |
| institution | Directory Open Access Journal |
| issn | 2590-0064 |
| language | English |
| last_indexed | 2024-03-08T14:07:49Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj.art-303dd0863eb34f63a40a800b264b8ef12024-01-15T04:24:04ZengElsevierMaterials Today Bio2590-00642024-02-0124100929DLP fabrication of HA scaffold with customized porous structures to regulate immune microenvironment and macrophage polarization for enhancing bone regenerationShilang Xiong0Yinuo Zhang1Jianhua Zeng2Jingyu Zhou3Shiwei Liu4Peng Wei5Hantian Liu6Feng Yi7Zongmiao Wan8Long Xiong9Bin Zhang10Jingtang Li11Department of Orthopedics, First Affiliated Hospital of Nanchang University, No. 17 Yong Wai Zheng Street, Nanchang, Jiangxi, 330006, ChinaDepartment of Orthopedics, Huashan Hospital, Fudan University, 12 Middle Wulumuqi Road, Shanghai, 200040, ChinaDepartment of Spine Surgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, 200092, ChinaDepartment of Orthopedics, Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, ChinaDepartment of Orthopedics, Ganzhou People's Hospital No.16, Mei Guan Road, Zhang Gong District, Ganzhou, Jiangxi, 341000, ChinaDepartment of Orthopedics, Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, ChinaDepartment of Orthopedics, Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, ChinaDepartment of Orthopedics, Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, ChinaDepartment of Orthopedics, First Affiliated Hospital of Nanchang University, No. 17 Yong Wai Zheng Street, Nanchang, Jiangxi, 330006, ChinaDepartment of Orthopedics, Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, China; Corresponding author.Department of Orthopedics, First Affiliated Hospital of Nanchang University, No. 17 Yong Wai Zheng Street, Nanchang, Jiangxi, 330006, China; Corresponding author. Department of Orthopedics, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, China.Department of Traumatology, Jiangxi Provincial People's Hospital the First Affiliated Hospital of Nanchang Medical College, Nanchang, Jiangxi, 330006, China; Corresponding author.The immune microenvironment plays a pivotal role in osteoanagenesis. Biomaterials can modulate osteogenic efficacy by inducing specific local immune reactions. As 3D-printing technology advances, digital light projection printing has emerged as a promising method for creating large scale, high-precision biomaterial scaffolds. By adjusting the solid content and the sintering conditions during printing, the pore size of biomaterials can be meticulously controlled. Yet, the systematic influence of pore size on the immune microenvironment remains uncharted. We fabricated 3D-printed hydroxyapatite bioceramic scaffolds with three distinct pore sizes: 400 μm, 600 μm, and 800 μm. Our study revealed that scaffolds with a pore size of 600 μm promote macrophage M2 polarization, which is achieved by upregulating interferon−beta and HIF-1α production. When these materials were implanted subcutaneously in rats and within rabbit skulls, we observed that the 600 μm scaffolds notably improved the long-term inflammatory response, fostered vascular proliferation, and augmented new bone growth. This research paves the way for innovative therapeutic strategies for treating large segmental bone defects in clinical settings.http://www.sciencedirect.com/science/article/pii/S2590006423003897Bone regenerationImmunological environment3D printing technologyDigital light projectionPore size |
| spellingShingle | Shilang Xiong Yinuo Zhang Jianhua Zeng Jingyu Zhou Shiwei Liu Peng Wei Hantian Liu Feng Yi Zongmiao Wan Long Xiong Bin Zhang Jingtang Li DLP fabrication of HA scaffold with customized porous structures to regulate immune microenvironment and macrophage polarization for enhancing bone regeneration Materials Today Bio Bone regeneration Immunological environment 3D printing technology Digital light projection Pore size |
| title | DLP fabrication of HA scaffold with customized porous structures to regulate immune microenvironment and macrophage polarization for enhancing bone regeneration |
| title_full | DLP fabrication of HA scaffold with customized porous structures to regulate immune microenvironment and macrophage polarization for enhancing bone regeneration |
| title_fullStr | DLP fabrication of HA scaffold with customized porous structures to regulate immune microenvironment and macrophage polarization for enhancing bone regeneration |
| title_full_unstemmed | DLP fabrication of HA scaffold with customized porous structures to regulate immune microenvironment and macrophage polarization for enhancing bone regeneration |
| title_short | DLP fabrication of HA scaffold with customized porous structures to regulate immune microenvironment and macrophage polarization for enhancing bone regeneration |
| title_sort | dlp fabrication of ha scaffold with customized porous structures to regulate immune microenvironment and macrophage polarization for enhancing bone regeneration |
| topic | Bone regeneration Immunological environment 3D printing technology Digital light projection Pore size |
| url | http://www.sciencedirect.com/science/article/pii/S2590006423003897 |
| work_keys_str_mv | AT shilangxiong dlpfabricationofhascaffoldwithcustomizedporousstructurestoregulateimmunemicroenvironmentandmacrophagepolarizationforenhancingboneregeneration AT yinuozhang dlpfabricationofhascaffoldwithcustomizedporousstructurestoregulateimmunemicroenvironmentandmacrophagepolarizationforenhancingboneregeneration AT jianhuazeng dlpfabricationofhascaffoldwithcustomizedporousstructurestoregulateimmunemicroenvironmentandmacrophagepolarizationforenhancingboneregeneration AT jingyuzhou dlpfabricationofhascaffoldwithcustomizedporousstructurestoregulateimmunemicroenvironmentandmacrophagepolarizationforenhancingboneregeneration AT shiweiliu dlpfabricationofhascaffoldwithcustomizedporousstructurestoregulateimmunemicroenvironmentandmacrophagepolarizationforenhancingboneregeneration AT pengwei dlpfabricationofhascaffoldwithcustomizedporousstructurestoregulateimmunemicroenvironmentandmacrophagepolarizationforenhancingboneregeneration AT hantianliu dlpfabricationofhascaffoldwithcustomizedporousstructurestoregulateimmunemicroenvironmentandmacrophagepolarizationforenhancingboneregeneration AT fengyi dlpfabricationofhascaffoldwithcustomizedporousstructurestoregulateimmunemicroenvironmentandmacrophagepolarizationforenhancingboneregeneration AT zongmiaowan dlpfabricationofhascaffoldwithcustomizedporousstructurestoregulateimmunemicroenvironmentandmacrophagepolarizationforenhancingboneregeneration AT longxiong dlpfabricationofhascaffoldwithcustomizedporousstructurestoregulateimmunemicroenvironmentandmacrophagepolarizationforenhancingboneregeneration AT binzhang dlpfabricationofhascaffoldwithcustomizedporousstructurestoregulateimmunemicroenvironmentandmacrophagepolarizationforenhancingboneregeneration AT jingtangli dlpfabricationofhascaffoldwithcustomizedporousstructurestoregulateimmunemicroenvironmentandmacrophagepolarizationforenhancingboneregeneration |