Protocol for isolating small cytosolic dsDNA from cultured murine cells

Summary: We recently identified a class of small cytosolic double-stranded DNA (scDNA) approximately 20–40 bp in size in human and mouse cells. Here, we present a protocol for scDNA isolation from cultured murine cells. We describe steps for cytosolic compartment separation, DNA isolation in the cyt...

Fuld beskrivelse

Bibliografiske detaljer
Main Authors: Zhizheng Dai, Hongyu Ji, Anqi Zheng, Ai-Long Huang, Kai-Fu Tang
Format: Article
Sprog:English
Udgivet: Elsevier 2024-06-01
Serier:STAR Protocols
Fag:
Online adgang:http://www.sciencedirect.com/science/article/pii/S2666166724001631