Lycopene Improves In Vitro Development of Porcine Embryos by Reducing Oxidative Stress and Apoptosis
In vitro culture (IVC) for porcine embryo development is inferior compared to in vivo development because oxidative stress can be induced by the production of excessive reactive oxygen species (ROS) under high oxygen tension in the in vitro environment. To overcome this problem, we investigated the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/2/230 |
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| author | Hyo-Gu Kang Sanghoon Lee Pil-Soo Jeong Min Ju Kim Soo-Hyun Park Ye Eun Joo Sung Hyun Park Bong-Seok Song Sun-Uk Kim Min Kyu Kim Bo-Woong Sim |
| author_facet | Hyo-Gu Kang Sanghoon Lee Pil-Soo Jeong Min Ju Kim Soo-Hyun Park Ye Eun Joo Sung Hyun Park Bong-Seok Song Sun-Uk Kim Min Kyu Kim Bo-Woong Sim |
| author_sort | Hyo-Gu Kang |
| collection | DOAJ |
| description | In vitro culture (IVC) for porcine embryo development is inferior compared to in vivo development because oxidative stress can be induced by the production of excessive reactive oxygen species (ROS) under high oxygen tension in the in vitro environment. To overcome this problem, we investigated the effect of lycopene, an antioxidant carotenoid, on developmental competence and the mechanisms involved in mitochondria-dependent apoptosis pathways in porcine embryos. In vitro fertilized (IVF) embryos were cultured in IVC medium supplemented with 0, 0.02, 0.05, 0.1, or 0.2 μM lycopene. The results indicate that 0.1 μM lycopene significantly increased the rate of blastocyst formation and the total cell numbers, including trophectoderm cell numbers, on Day In terms of mitochondria-dependent apoptosis, IVF embryos treated with 0.1 μM lycopene exhibited significantly decreased levels of ROS, increased mitochondrial membrane potential, and decreased expression of cytochrome c on Days 2 and Furthermore, 0.1 μM lycopene significantly decreased the number and percentage of caspase 3-positive and apoptotic cells in Day-6 blastocysts. In addition, Day-2 embryos and Day-6 blastocysts treated with 0.1 μM lycopene showed significantly reduced mRNA expression related to antioxidant enzymes (<i>SOD1, SOD2, CATALASE</i>) and apoptosis (<i>BAX/BCL2L1</i> ratio). These results indicate that lycopene supplementation during the entire period of IVC enhanced embryonic development in pigs by regulating oxidative stress and mitochondria-dependent apoptosis. |
| first_indexed | 2024-03-09T05:51:03Z |
| format | Article |
| id | doaj.art-30404a924b9a491188f0aaf99ec86d78 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T05:51:03Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-30404a924b9a491188f0aaf99ec86d782023-12-03T12:17:00ZengMDPI AGAntioxidants2076-39212021-02-0110223010.3390/antiox10020230Lycopene Improves In Vitro Development of Porcine Embryos by Reducing Oxidative Stress and ApoptosisHyo-Gu Kang0Sanghoon Lee1Pil-Soo Jeong2Min Ju Kim3Soo-Hyun Park4Ye Eun Joo5Sung Hyun Park6Bong-Seok Song7Sun-Uk Kim8Min Kyu Kim9Bo-Woong Sim10Futuristic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungcheongbuk-do, Cheongju 28116, KoreaFuturistic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungcheongbuk-do, Cheongju 28116, KoreaFuturistic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungcheongbuk-do, Cheongju 28116, KoreaFuturistic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungcheongbuk-do, Cheongju 28116, KoreaFuturistic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungcheongbuk-do, Cheongju 28116, KoreaFuturistic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungcheongbuk-do, Cheongju 28116, KoreaFuturistic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungcheongbuk-do, Cheongju 28116, KoreaFuturistic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungcheongbuk-do, Cheongju 28116, KoreaFuturistic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungcheongbuk-do, Cheongju 28116, KoreaLaboratory of Animal Reproduction and Physiology, Department of Animal Science and Biotechnology, College of Agriculture and Life Science, Chungnam National University, Daejeon 34134, KoreaFuturistic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Chungcheongbuk-do, Cheongju 28116, KoreaIn vitro culture (IVC) for porcine embryo development is inferior compared to in vivo development because oxidative stress can be induced by the production of excessive reactive oxygen species (ROS) under high oxygen tension in the in vitro environment. To overcome this problem, we investigated the effect of lycopene, an antioxidant carotenoid, on developmental competence and the mechanisms involved in mitochondria-dependent apoptosis pathways in porcine embryos. In vitro fertilized (IVF) embryos were cultured in IVC medium supplemented with 0, 0.02, 0.05, 0.1, or 0.2 μM lycopene. The results indicate that 0.1 μM lycopene significantly increased the rate of blastocyst formation and the total cell numbers, including trophectoderm cell numbers, on Day In terms of mitochondria-dependent apoptosis, IVF embryos treated with 0.1 μM lycopene exhibited significantly decreased levels of ROS, increased mitochondrial membrane potential, and decreased expression of cytochrome c on Days 2 and Furthermore, 0.1 μM lycopene significantly decreased the number and percentage of caspase 3-positive and apoptotic cells in Day-6 blastocysts. In addition, Day-2 embryos and Day-6 blastocysts treated with 0.1 μM lycopene showed significantly reduced mRNA expression related to antioxidant enzymes (<i>SOD1, SOD2, CATALASE</i>) and apoptosis (<i>BAX/BCL2L1</i> ratio). These results indicate that lycopene supplementation during the entire period of IVC enhanced embryonic development in pigs by regulating oxidative stress and mitochondria-dependent apoptosis.https://www.mdpi.com/2076-3921/10/2/230lycopeneantioxidantreactive oxygen speciesmitochondria-dependent apoptosispigreproduction |
| spellingShingle | Hyo-Gu Kang Sanghoon Lee Pil-Soo Jeong Min Ju Kim Soo-Hyun Park Ye Eun Joo Sung Hyun Park Bong-Seok Song Sun-Uk Kim Min Kyu Kim Bo-Woong Sim Lycopene Improves In Vitro Development of Porcine Embryos by Reducing Oxidative Stress and Apoptosis Antioxidants lycopene antioxidant reactive oxygen species mitochondria-dependent apoptosis pig reproduction |
| title | Lycopene Improves In Vitro Development of Porcine Embryos by Reducing Oxidative Stress and Apoptosis |
| title_full | Lycopene Improves In Vitro Development of Porcine Embryos by Reducing Oxidative Stress and Apoptosis |
| title_fullStr | Lycopene Improves In Vitro Development of Porcine Embryos by Reducing Oxidative Stress and Apoptosis |
| title_full_unstemmed | Lycopene Improves In Vitro Development of Porcine Embryos by Reducing Oxidative Stress and Apoptosis |
| title_short | Lycopene Improves In Vitro Development of Porcine Embryos by Reducing Oxidative Stress and Apoptosis |
| title_sort | lycopene improves in vitro development of porcine embryos by reducing oxidative stress and apoptosis |
| topic | lycopene antioxidant reactive oxygen species mitochondria-dependent apoptosis pig reproduction |
| url | https://www.mdpi.com/2076-3921/10/2/230 |
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