Identification of metabolite and protein explanatory variables governing microbiome establishment and re-establishment within a cellulose-degrading anaerobic bioreactor.
Proteins, metabolites, and 16S rRNA measurements were used to examine the community structure and functional relationships within a cellulose degrading anaerobic bioreactor. The bioreactor was seeded with bovine rumen fluid and operated with a 4 day hydraulic retention time on cellulose (avicel) as...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC6173382?pdf=render |
| _version_ | 1818431854635646976 |
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| author | Stephen J Callister Lee Ann McCue Amy A Boaro Brian LaMarche Richard A White Joseph M Brown Birgitte K Ahring |
| author_facet | Stephen J Callister Lee Ann McCue Amy A Boaro Brian LaMarche Richard A White Joseph M Brown Birgitte K Ahring |
| author_sort | Stephen J Callister |
| collection | DOAJ |
| description | Proteins, metabolites, and 16S rRNA measurements were used to examine the community structure and functional relationships within a cellulose degrading anaerobic bioreactor. The bioreactor was seeded with bovine rumen fluid and operated with a 4 day hydraulic retention time on cellulose (avicel) as sole carbon and energy source. The reactor performance and microbial community structure was monitored during the establishment of the cellulose-degrading community. After stable operation was established in the bioreactor, the mixing intensity was increased in order to investigate the effect of a physical disruption of the microbial community structure. Finally, the original conditions were re-established to understand the stability of the microbial community after a perturbation. All factors measured were found to be inter-correlated during these three distinct phases of operation (establishment, perturbation and re-establishment). In particular, the return of community structure and function to pre-perturbed conditions suggests that propionate fermentation and acetate utilization were the explanatory factors for community establishment and re-establishment. |
| first_indexed | 2024-12-14T15:55:55Z |
| format | Article |
| id | doaj.art-304337722d6045e3b34fdedac7d9d9ad |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T15:55:55Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-304337722d6045e3b34fdedac7d9d9ad2022-12-21T22:55:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-011310e020483110.1371/journal.pone.0204831Identification of metabolite and protein explanatory variables governing microbiome establishment and re-establishment within a cellulose-degrading anaerobic bioreactor.Stephen J CallisterLee Ann McCueAmy A BoaroBrian LaMarcheRichard A WhiteJoseph M BrownBirgitte K AhringProteins, metabolites, and 16S rRNA measurements were used to examine the community structure and functional relationships within a cellulose degrading anaerobic bioreactor. The bioreactor was seeded with bovine rumen fluid and operated with a 4 day hydraulic retention time on cellulose (avicel) as sole carbon and energy source. The reactor performance and microbial community structure was monitored during the establishment of the cellulose-degrading community. After stable operation was established in the bioreactor, the mixing intensity was increased in order to investigate the effect of a physical disruption of the microbial community structure. Finally, the original conditions were re-established to understand the stability of the microbial community after a perturbation. All factors measured were found to be inter-correlated during these three distinct phases of operation (establishment, perturbation and re-establishment). In particular, the return of community structure and function to pre-perturbed conditions suggests that propionate fermentation and acetate utilization were the explanatory factors for community establishment and re-establishment.http://europepmc.org/articles/PMC6173382?pdf=render |
| spellingShingle | Stephen J Callister Lee Ann McCue Amy A Boaro Brian LaMarche Richard A White Joseph M Brown Birgitte K Ahring Identification of metabolite and protein explanatory variables governing microbiome establishment and re-establishment within a cellulose-degrading anaerobic bioreactor. PLoS ONE |
| title | Identification of metabolite and protein explanatory variables governing microbiome establishment and re-establishment within a cellulose-degrading anaerobic bioreactor. |
| title_full | Identification of metabolite and protein explanatory variables governing microbiome establishment and re-establishment within a cellulose-degrading anaerobic bioreactor. |
| title_fullStr | Identification of metabolite and protein explanatory variables governing microbiome establishment and re-establishment within a cellulose-degrading anaerobic bioreactor. |
| title_full_unstemmed | Identification of metabolite and protein explanatory variables governing microbiome establishment and re-establishment within a cellulose-degrading anaerobic bioreactor. |
| title_short | Identification of metabolite and protein explanatory variables governing microbiome establishment and re-establishment within a cellulose-degrading anaerobic bioreactor. |
| title_sort | identification of metabolite and protein explanatory variables governing microbiome establishment and re establishment within a cellulose degrading anaerobic bioreactor |
| url | http://europepmc.org/articles/PMC6173382?pdf=render |
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