Predictive value of TRPV2 expression from peripheral blood mononuclear cells on the early recurrence of atrial fibrillation after radiofrequency catheter ablation
Abstract Background Recent study has shown that the transient receptor potential vanilloid 2 (TRPV2) channel was exclusively upregulated in patients with atrial fibrillation (AF), and that this overexpression might be detrimental for occurrence and maintenance of AF. We aimed to characterize the exp...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-12-01
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| Series: | BMC Cardiovascular Disorders |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12872-022-02992-0 |
| _version_ | 1797980136548597760 |
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| author | Xuebin Ling Jun Wang Xue Qin Chufen Lin Wei Jie Yane Chen Dajia Fu Yang Yang Qingwen Meng Jing Lin Hui Liu Tianfa Li Junli Guo |
| author_facet | Xuebin Ling Jun Wang Xue Qin Chufen Lin Wei Jie Yane Chen Dajia Fu Yang Yang Qingwen Meng Jing Lin Hui Liu Tianfa Li Junli Guo |
| author_sort | Xuebin Ling |
| collection | DOAJ |
| description | Abstract Background Recent study has shown that the transient receptor potential vanilloid 2 (TRPV2) channel was exclusively upregulated in patients with atrial fibrillation (AF), and that this overexpression might be detrimental for occurrence and maintenance of AF. We aimed to characterize the expression levels of TRPV2 mRNA in peripheral blood mononuclear cells (PBMCs) with/without early recurrence of atrial fibrillation (ERAF) after radiofrequency catheter ablation (RFCA), and to find a reliable predictor for ERAF. Methods 65 patients of AF, who underwent RFCA successfully, then divided into two groups according to ERAF during following 3 months. PBMCs were isolated from whole blood by Ficoll gradient centrifugation before and after RFCA. Gene set enrichment analysis was performed to evaluate TRPV channels expression levels and Kyoto Encyclopedia of Genes and Genomes (KEGG) mapping was used for pathway enrichment analysis. Results There was no significant difference in the TRPV2 mRNA expression level between the two groups before RFCA, while without ERAF group of TRPV2 expression was markedly reduced compared to ERAF group after RFCA. Moreover, the number of TRPV2 expression was confirmed as an independent predictor for the first time through receiver operating characteristic and Kaplan–Meier survival curve analysis. It should be pointed out that the above results were only used to predict ERAF, and have no predictive significance for late recurrence of atrial fibrillation according to the current data. Additionally, ERAF was inversely correlated with P wave dispersion. KEGG mapping further clustered 41 pathways, revealing that ‘‘cyclic guanosine monophosphate-protein kinase G signaling pathway’’ was significantly enriched. Conclusions We firstly assume that downregulated expression of peripheral TRPV2 appear in patients without ERAF after RFCA. TRPV2 may thus represent a novel predictor of early phase after successful radiofrequency ablation. |
| first_indexed | 2024-04-11T05:50:50Z |
| format | Article |
| id | doaj.art-3045ad62951543f2858d71853a04b7ae |
| institution | Directory Open Access Journal |
| issn | 1471-2261 |
| language | English |
| last_indexed | 2024-04-11T05:50:50Z |
| publishDate | 2022-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cardiovascular Disorders |
| spelling | doaj.art-3045ad62951543f2858d71853a04b7ae2022-12-22T04:42:05ZengBMCBMC Cardiovascular Disorders1471-22612022-12-0122111310.1186/s12872-022-02992-0Predictive value of TRPV2 expression from peripheral blood mononuclear cells on the early recurrence of atrial fibrillation after radiofrequency catheter ablationXuebin Ling0Jun Wang1Xue Qin2Chufen Lin3Wei Jie4Yane Chen5Dajia Fu6Yang Yang7Qingwen Meng8Jing Lin9Hui Liu10Tianfa Li11Junli Guo12Key Laboratory of Tropical Cardiovascular Diseases Research of Hainan Province, Cardiovascular Diseases Institute of the First Affiliated Hospital, Department of Cardiovascular Surgery, the Second Affiliated Hospital of Hainan Medical University, Key Laboratory of Emergency and Trauma of Ministry of Education, Hainan Medical UniversityKey Laboratory of Tropical Cardiovascular Diseases Research of Hainan Province, Cardiovascular Diseases Institute of the First Affiliated Hospital, Department of Cardiovascular Surgery, the Second Affiliated Hospital of Hainan Medical University, Key Laboratory of Emergency and Trauma of Ministry of Education, Hainan Medical UniversityKey Laboratory of Tropical Cardiovascular Diseases Research of Hainan Province, Cardiovascular Diseases Institute of the First Affiliated Hospital, Department of Cardiovascular Surgery, the Second Affiliated Hospital of Hainan Medical University, Key Laboratory of Emergency and Trauma of Ministry of Education, Hainan Medical UniversityDepartment of Health Medicine, Affiliated Haikou Hospital of Xiangya Medical College, Central South UniversityKey Laboratory of Tropical Cardiovascular Diseases Research of Hainan Province, Cardiovascular Diseases Institute of the First Affiliated Hospital, Department of Cardiovascular Surgery, the Second Affiliated Hospital of Hainan Medical University, Key Laboratory of Emergency and Trauma of Ministry of Education, Hainan Medical UniversityKey Laboratory of Tropical Cardiovascular Diseases Research of Hainan Province, Cardiovascular Diseases Institute of the First Affiliated Hospital, Department of Cardiovascular Surgery, the Second Affiliated Hospital of Hainan Medical University, Key Laboratory of Emergency and Trauma of Ministry of Education, Hainan Medical UniversityKey Laboratory of Tropical Cardiovascular Diseases Research of Hainan Province, Cardiovascular Diseases Institute of the First Affiliated Hospital, Department of Cardiovascular Surgery, the Second Affiliated Hospital of Hainan Medical University, Key Laboratory of Emergency and Trauma of Ministry of Education, Hainan Medical UniversityKey Laboratory of Tropical Cardiovascular Diseases Research of Hainan Province, Cardiovascular Diseases Institute of the First Affiliated Hospital, Department of Cardiovascular Surgery, the Second Affiliated Hospital of Hainan Medical University, Key Laboratory of Emergency and Trauma of Ministry of Education, Hainan Medical UniversityKey Laboratory of Tropical Cardiovascular Diseases Research of Hainan Province, Cardiovascular Diseases Institute of the First Affiliated Hospital, Department of Cardiovascular Surgery, the Second Affiliated Hospital of Hainan Medical University, Key Laboratory of Emergency and Trauma of Ministry of Education, Hainan Medical UniversityKey Laboratory of Tropical Cardiovascular Diseases Research of Hainan Province, Cardiovascular Diseases Institute of the First Affiliated Hospital, Department of Cardiovascular Surgery, the Second Affiliated Hospital of Hainan Medical University, Key Laboratory of Emergency and Trauma of Ministry of Education, Hainan Medical UniversityDepartment of Anatomy, School of Basic Medicine and Life Science, Hainan Medical UniversityKey Laboratory of Tropical Cardiovascular Diseases Research of Hainan Province, Cardiovascular Diseases Institute of the First Affiliated Hospital, Department of Cardiovascular Surgery, the Second Affiliated Hospital of Hainan Medical University, Key Laboratory of Emergency and Trauma of Ministry of Education, Hainan Medical UniversityKey Laboratory of Tropical Cardiovascular Diseases Research of Hainan Province, Cardiovascular Diseases Institute of the First Affiliated Hospital, Department of Cardiovascular Surgery, the Second Affiliated Hospital of Hainan Medical University, Key Laboratory of Emergency and Trauma of Ministry of Education, Hainan Medical UniversityAbstract Background Recent study has shown that the transient receptor potential vanilloid 2 (TRPV2) channel was exclusively upregulated in patients with atrial fibrillation (AF), and that this overexpression might be detrimental for occurrence and maintenance of AF. We aimed to characterize the expression levels of TRPV2 mRNA in peripheral blood mononuclear cells (PBMCs) with/without early recurrence of atrial fibrillation (ERAF) after radiofrequency catheter ablation (RFCA), and to find a reliable predictor for ERAF. Methods 65 patients of AF, who underwent RFCA successfully, then divided into two groups according to ERAF during following 3 months. PBMCs were isolated from whole blood by Ficoll gradient centrifugation before and after RFCA. Gene set enrichment analysis was performed to evaluate TRPV channels expression levels and Kyoto Encyclopedia of Genes and Genomes (KEGG) mapping was used for pathway enrichment analysis. Results There was no significant difference in the TRPV2 mRNA expression level between the two groups before RFCA, while without ERAF group of TRPV2 expression was markedly reduced compared to ERAF group after RFCA. Moreover, the number of TRPV2 expression was confirmed as an independent predictor for the first time through receiver operating characteristic and Kaplan–Meier survival curve analysis. It should be pointed out that the above results were only used to predict ERAF, and have no predictive significance for late recurrence of atrial fibrillation according to the current data. Additionally, ERAF was inversely correlated with P wave dispersion. KEGG mapping further clustered 41 pathways, revealing that ‘‘cyclic guanosine monophosphate-protein kinase G signaling pathway’’ was significantly enriched. Conclusions We firstly assume that downregulated expression of peripheral TRPV2 appear in patients without ERAF after RFCA. TRPV2 may thus represent a novel predictor of early phase after successful radiofrequency ablation.https://doi.org/10.1186/s12872-022-02992-0TRPV2Peripheral blood mononuclear cells (PBMCs)Early recurrenceAtrial fibrillation |
| spellingShingle | Xuebin Ling Jun Wang Xue Qin Chufen Lin Wei Jie Yane Chen Dajia Fu Yang Yang Qingwen Meng Jing Lin Hui Liu Tianfa Li Junli Guo Predictive value of TRPV2 expression from peripheral blood mononuclear cells on the early recurrence of atrial fibrillation after radiofrequency catheter ablation BMC Cardiovascular Disorders TRPV2 Peripheral blood mononuclear cells (PBMCs) Early recurrence Atrial fibrillation |
| title | Predictive value of TRPV2 expression from peripheral blood mononuclear cells on the early recurrence of atrial fibrillation after radiofrequency catheter ablation |
| title_full | Predictive value of TRPV2 expression from peripheral blood mononuclear cells on the early recurrence of atrial fibrillation after radiofrequency catheter ablation |
| title_fullStr | Predictive value of TRPV2 expression from peripheral blood mononuclear cells on the early recurrence of atrial fibrillation after radiofrequency catheter ablation |
| title_full_unstemmed | Predictive value of TRPV2 expression from peripheral blood mononuclear cells on the early recurrence of atrial fibrillation after radiofrequency catheter ablation |
| title_short | Predictive value of TRPV2 expression from peripheral blood mononuclear cells on the early recurrence of atrial fibrillation after radiofrequency catheter ablation |
| title_sort | predictive value of trpv2 expression from peripheral blood mononuclear cells on the early recurrence of atrial fibrillation after radiofrequency catheter ablation |
| topic | TRPV2 Peripheral blood mononuclear cells (PBMCs) Early recurrence Atrial fibrillation |
| url | https://doi.org/10.1186/s12872-022-02992-0 |
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