A Comparison of RNA Interference via Injection and Feeding in Honey Bees
RNA interference (RNAi) has been used successfully to reduce target gene expression and induce specific phenotypes in several species. It has proved useful as a tool to investigate gene function and has the potential to manage pest populations and reduce disease pathogens. However, it is not known w...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/13/10/928 |
| _version_ | 1797472503824646144 |
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| author | Yong Zhang Zhen Li Zi-Long Wang Li-Zhen Zhang Zhi-Jiang Zeng |
| author_facet | Yong Zhang Zhen Li Zi-Long Wang Li-Zhen Zhang Zhi-Jiang Zeng |
| author_sort | Yong Zhang |
| collection | DOAJ |
| description | RNA interference (RNAi) has been used successfully to reduce target gene expression and induce specific phenotypes in several species. It has proved useful as a tool to investigate gene function and has the potential to manage pest populations and reduce disease pathogens. However, it is not known whether different administration methods are equally effective at interfering with genes in bees. Therefore, we compared the effects of feeding and injection of small interfering RNA (siRNA) on the messenger RNA (mRNA) levels of alpha-aminoadipic semialdehyde dehydrogenase (<i>ALDH7A1</i>), 4-coumarate-CoA ligase (<i>4CL</i>), and heat shock protein 70 (<i>HSP70</i>). Both feeding and injection of siRNA successfully knocked down the gene but feeding required more siRNA than the injection. Our results suggest that both feeding and injection of siRNA effectively interfere with brain genes in bees. The appropriateness of each method would depend on the situation. |
| first_indexed | 2024-03-09T20:03:38Z |
| format | Article |
| id | doaj.art-304a61e78e7248279b7d14c8cc5364f3 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-09T20:03:38Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-304a61e78e7248279b7d14c8cc5364f32023-11-24T00:38:13ZengMDPI AGInsects2075-44502022-10-01131092810.3390/insects13100928A Comparison of RNA Interference via Injection and Feeding in Honey BeesYong Zhang0Zhen Li1Zi-Long Wang2Li-Zhen Zhang3Zhi-Jiang Zeng4Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, ChinaHoneybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, ChinaHoneybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, ChinaHoneybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, ChinaHoneybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, ChinaRNA interference (RNAi) has been used successfully to reduce target gene expression and induce specific phenotypes in several species. It has proved useful as a tool to investigate gene function and has the potential to manage pest populations and reduce disease pathogens. However, it is not known whether different administration methods are equally effective at interfering with genes in bees. Therefore, we compared the effects of feeding and injection of small interfering RNA (siRNA) on the messenger RNA (mRNA) levels of alpha-aminoadipic semialdehyde dehydrogenase (<i>ALDH7A1</i>), 4-coumarate-CoA ligase (<i>4CL</i>), and heat shock protein 70 (<i>HSP70</i>). Both feeding and injection of siRNA successfully knocked down the gene but feeding required more siRNA than the injection. Our results suggest that both feeding and injection of siRNA effectively interfere with brain genes in bees. The appropriateness of each method would depend on the situation.https://www.mdpi.com/2075-4450/13/10/928RNAisiRNAhoney beebrain |
| spellingShingle | Yong Zhang Zhen Li Zi-Long Wang Li-Zhen Zhang Zhi-Jiang Zeng A Comparison of RNA Interference via Injection and Feeding in Honey Bees Insects RNAi siRNA honey bee brain |
| title | A Comparison of RNA Interference via Injection and Feeding in Honey Bees |
| title_full | A Comparison of RNA Interference via Injection and Feeding in Honey Bees |
| title_fullStr | A Comparison of RNA Interference via Injection and Feeding in Honey Bees |
| title_full_unstemmed | A Comparison of RNA Interference via Injection and Feeding in Honey Bees |
| title_short | A Comparison of RNA Interference via Injection and Feeding in Honey Bees |
| title_sort | comparison of rna interference via injection and feeding in honey bees |
| topic | RNAi siRNA honey bee brain |
| url | https://www.mdpi.com/2075-4450/13/10/928 |
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