Urinary peptidomics reveals proteases involved in idiopathic membranous nephropathy
Abstract Background Idiopathic membranous nephropathy (IMN) is a cause of nephrotic syndrome that is increasing in incidence but has unclear pathogenesis. Urinary peptidomics is a promising technology for elucidating molecular mechanisms underlying diseases. Dysregulation of the proteolytic system i...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-11-01
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| Series: | BMC Genomics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12864-021-08155-3 |
| _version_ | 1818741140112801792 |
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| author | Baoxu Lin Jianhua Liu Yue Zhang Yabin Wu Shixiao Chen Yibo Bai Qiuying Liu Xiaosong Qin |
| author_facet | Baoxu Lin Jianhua Liu Yue Zhang Yabin Wu Shixiao Chen Yibo Bai Qiuying Liu Xiaosong Qin |
| author_sort | Baoxu Lin |
| collection | DOAJ |
| description | Abstract Background Idiopathic membranous nephropathy (IMN) is a cause of nephrotic syndrome that is increasing in incidence but has unclear pathogenesis. Urinary peptidomics is a promising technology for elucidating molecular mechanisms underlying diseases. Dysregulation of the proteolytic system is implicated in various diseases. Here, we aimed to conduct urinary peptidomics to identify IMN-related proteases. Results Peptide fingerprints indicated differences in naturally produced urinary peptide components among 20 healthy individuals, 22 patients with IMN, and 15 patients with other kidney diseases. In total, 1,080 peptide-matched proteins were identified, 279 proteins differentially expressed in the urine of IMN patients were screened, and 32 proteases were predicted; 55 of the matched proteins were also differentially expressed in the kidney tissues of IMN patients, and these were mainly involved in the regulation of proteasome-, lysosome-, and actin cytoskeleton-related signaling pathways. The 32 predicted proteases showed abnormal expression in the glomeruli of IMN patients based on Gene Expression Omnibus databases. Western blot revealed abnormal expression of calpain, matrix metalloproteinase 14, and cathepsin S in kidney tissues of patients with IMN. Conclusions This work shown the calpain/matrix metalloproteinase/cathepsin axis might be dysregulated in IMN. Our study is the first to systematically explore the role of proteases in IMN by urinary peptidomics, which are expected to facilitate discovery of better biomarkers for IMN. |
| first_indexed | 2024-12-18T01:51:53Z |
| format | Article |
| id | doaj.art-304b1ce3c3e740239e3d34ff25cd62ed |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-18T01:51:53Z |
| publishDate | 2021-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-304b1ce3c3e740239e3d34ff25cd62ed2022-12-21T21:25:00ZengBMCBMC Genomics1471-21642021-11-0122111210.1186/s12864-021-08155-3Urinary peptidomics reveals proteases involved in idiopathic membranous nephropathyBaoxu Lin0Jianhua Liu1Yue Zhang2Yabin Wu3Shixiao Chen4Yibo Bai5Qiuying Liu6Xiaosong Qin7Department of Laboratory Medicine, Shengjing Hospital of China Medical UniversityDepartment of Laboratory Medicine, Shengjing Hospital of China Medical UniversityDepartment of Laboratory Medicine, Shengjing Hospital of China Medical UniversityDepartment of Laboratory Medicine, Shengjing Hospital of China Medical UniversityDepartment of Laboratory Medicine, Shengjing Hospital of China Medical UniversityDepartment of Laboratory Medicine, Shengjing Hospital of China Medical UniversityDepartment of Laboratory Medicine, Shengjing Hospital of China Medical UniversityDepartment of Laboratory Medicine, Shengjing Hospital of China Medical UniversityAbstract Background Idiopathic membranous nephropathy (IMN) is a cause of nephrotic syndrome that is increasing in incidence but has unclear pathogenesis. Urinary peptidomics is a promising technology for elucidating molecular mechanisms underlying diseases. Dysregulation of the proteolytic system is implicated in various diseases. Here, we aimed to conduct urinary peptidomics to identify IMN-related proteases. Results Peptide fingerprints indicated differences in naturally produced urinary peptide components among 20 healthy individuals, 22 patients with IMN, and 15 patients with other kidney diseases. In total, 1,080 peptide-matched proteins were identified, 279 proteins differentially expressed in the urine of IMN patients were screened, and 32 proteases were predicted; 55 of the matched proteins were also differentially expressed in the kidney tissues of IMN patients, and these were mainly involved in the regulation of proteasome-, lysosome-, and actin cytoskeleton-related signaling pathways. The 32 predicted proteases showed abnormal expression in the glomeruli of IMN patients based on Gene Expression Omnibus databases. Western blot revealed abnormal expression of calpain, matrix metalloproteinase 14, and cathepsin S in kidney tissues of patients with IMN. Conclusions This work shown the calpain/matrix metalloproteinase/cathepsin axis might be dysregulated in IMN. Our study is the first to systematically explore the role of proteases in IMN by urinary peptidomics, which are expected to facilitate discovery of better biomarkers for IMN.https://doi.org/10.1186/s12864-021-08155-3Urinary peptidomicsIdiopathic membranous nephropathyProteases |
| spellingShingle | Baoxu Lin Jianhua Liu Yue Zhang Yabin Wu Shixiao Chen Yibo Bai Qiuying Liu Xiaosong Qin Urinary peptidomics reveals proteases involved in idiopathic membranous nephropathy BMC Genomics Urinary peptidomics Idiopathic membranous nephropathy Proteases |
| title | Urinary peptidomics reveals proteases involved in idiopathic membranous nephropathy |
| title_full | Urinary peptidomics reveals proteases involved in idiopathic membranous nephropathy |
| title_fullStr | Urinary peptidomics reveals proteases involved in idiopathic membranous nephropathy |
| title_full_unstemmed | Urinary peptidomics reveals proteases involved in idiopathic membranous nephropathy |
| title_short | Urinary peptidomics reveals proteases involved in idiopathic membranous nephropathy |
| title_sort | urinary peptidomics reveals proteases involved in idiopathic membranous nephropathy |
| topic | Urinary peptidomics Idiopathic membranous nephropathy Proteases |
| url | https://doi.org/10.1186/s12864-021-08155-3 |
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