Transcriptome Analysis on the Mechanism of Ethylicin Inhibiting <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> on Kiwifruit
Bacterial canker disease caused by <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> (Psa) is a devastating disease of kiwifruit, which is severely limiting the development of the kiwifruit industry. Ethylicin is a broad-spectrum plant biomimetic fungicide. However, its appli...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-03-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/9/4/724 |
| _version_ | 1827696264587771904 |
|---|---|
| author | Tao Liu Xiaoli Ren Guangyun Cao Xia Zhou Linhong Jin |
| author_facet | Tao Liu Xiaoli Ren Guangyun Cao Xia Zhou Linhong Jin |
| author_sort | Tao Liu |
| collection | DOAJ |
| description | Bacterial canker disease caused by <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> (Psa) is a devastating disease of kiwifruit, which is severely limiting the development of the kiwifruit industry. Ethylicin is a broad-spectrum plant biomimetic fungicide. However, its application in the control of kiwifruit bacterial canker is rarely reported, and the mechanism of ethylicin on Psa remains unknown. In this study, we investigated the effect of ethylicin on Psa in vitro and in vivo and found that ethylicin can inhibit the growth of Psa and prevent the cankering in the plant stem. Mechanism investigation indicated that ethylicin acted by limiting the movement of Psa, destroying the cell membrane of Psa, and inhibiting the formation of Psa biofilm. In addition, it was also found through transcriptomics research that ethylicin can up-regulate the expression of genes related to protein export and biofilm formation–<i>Pseudomonas aeruginosa</i> and down-regulate the expression of genes related to flagellar assembly in Psa. This study concluded that ethylicin can effectively inhibit Psa growth, and it could help to gain a better understanding of the mechanisms of ethylicin inhibiting Psa and provide practical data for the application of ethylicin as a highly potent agent for controlling the bacterial canker disease of kiwifruit. |
| first_indexed | 2024-03-10T12:44:15Z |
| format | Article |
| id | doaj.art-304c8be9e3b84d8db3ca791e477547c0 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T12:44:15Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-304c8be9e3b84d8db3ca791e477547c02023-11-21T13:36:05ZengMDPI AGMicroorganisms2076-26072021-03-019472410.3390/microorganisms9040724Transcriptome Analysis on the Mechanism of Ethylicin Inhibiting <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> on KiwifruitTao Liu0Xiaoli Ren1Guangyun Cao2Xia Zhou3Linhong Jin4State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, ChinaState Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, ChinaState Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, ChinaState Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, ChinaState Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang 550025, ChinaBacterial canker disease caused by <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> (Psa) is a devastating disease of kiwifruit, which is severely limiting the development of the kiwifruit industry. Ethylicin is a broad-spectrum plant biomimetic fungicide. However, its application in the control of kiwifruit bacterial canker is rarely reported, and the mechanism of ethylicin on Psa remains unknown. In this study, we investigated the effect of ethylicin on Psa in vitro and in vivo and found that ethylicin can inhibit the growth of Psa and prevent the cankering in the plant stem. Mechanism investigation indicated that ethylicin acted by limiting the movement of Psa, destroying the cell membrane of Psa, and inhibiting the formation of Psa biofilm. In addition, it was also found through transcriptomics research that ethylicin can up-regulate the expression of genes related to protein export and biofilm formation–<i>Pseudomonas aeruginosa</i> and down-regulate the expression of genes related to flagellar assembly in Psa. This study concluded that ethylicin can effectively inhibit Psa growth, and it could help to gain a better understanding of the mechanisms of ethylicin inhibiting Psa and provide practical data for the application of ethylicin as a highly potent agent for controlling the bacterial canker disease of kiwifruit.https://www.mdpi.com/2076-2607/9/4/724kiwifruit bacterial canker<i>Pseudomonas syringae</i> pv. <i>actinidiae</i>ethylicinantibacterial actiontranscriptomeaction mechanism |
| spellingShingle | Tao Liu Xiaoli Ren Guangyun Cao Xia Zhou Linhong Jin Transcriptome Analysis on the Mechanism of Ethylicin Inhibiting <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> on Kiwifruit Microorganisms kiwifruit bacterial canker <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> ethylicin antibacterial action transcriptome action mechanism |
| title | Transcriptome Analysis on the Mechanism of Ethylicin Inhibiting <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> on Kiwifruit |
| title_full | Transcriptome Analysis on the Mechanism of Ethylicin Inhibiting <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> on Kiwifruit |
| title_fullStr | Transcriptome Analysis on the Mechanism of Ethylicin Inhibiting <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> on Kiwifruit |
| title_full_unstemmed | Transcriptome Analysis on the Mechanism of Ethylicin Inhibiting <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> on Kiwifruit |
| title_short | Transcriptome Analysis on the Mechanism of Ethylicin Inhibiting <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> on Kiwifruit |
| title_sort | transcriptome analysis on the mechanism of ethylicin inhibiting i pseudomonas syringae i pv i actinidiae i on kiwifruit |
| topic | kiwifruit bacterial canker <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> ethylicin antibacterial action transcriptome action mechanism |
| url | https://www.mdpi.com/2076-2607/9/4/724 |
| work_keys_str_mv | AT taoliu transcriptomeanalysisonthemechanismofethylicininhibitingipseudomonassyringaeipviactinidiaeionkiwifruit AT xiaoliren transcriptomeanalysisonthemechanismofethylicininhibitingipseudomonassyringaeipviactinidiaeionkiwifruit AT guangyuncao transcriptomeanalysisonthemechanismofethylicininhibitingipseudomonassyringaeipviactinidiaeionkiwifruit AT xiazhou transcriptomeanalysisonthemechanismofethylicininhibitingipseudomonassyringaeipviactinidiaeionkiwifruit AT linhongjin transcriptomeanalysisonthemechanismofethylicininhibitingipseudomonassyringaeipviactinidiaeionkiwifruit |