The Frequency and Antimicrobial Resistance of blaTEM and blaCTX-M Genes in Escherichia coli Isolated From Patients With a Urinary Tract Infection
Introduction: The production of β-lactamase in bacteria, especially in Escherichia coli as a prevalent opportunistic bacterium, has caused many problems in patient treatment. β-lactamases are encoded by extended-spectrum β-lactamase (ESBL) genes such as blaTEM and blaCTX-M. We aimed to assess the pr...
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| Format: | Article |
| Language: | English |
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Zabol University of Medical sciences
2020-06-01
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| Series: | International Journal of Basic Science in Medicine |
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| Online Access: | http://ijbsm.zbmu.ac.ir/PDF/ijbsm-13310 |
| _version_ | 1818856538527236096 |
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| author | Parisa Shahbazi Mohammad Jahantigh Saeed Salari Salehe Danesh |
| author_facet | Parisa Shahbazi Mohammad Jahantigh Saeed Salari Salehe Danesh |
| author_sort | Parisa Shahbazi |
| collection | DOAJ |
| description | Introduction: The production of β-lactamase in bacteria, especially in Escherichia coli as a prevalent opportunistic bacterium, has caused many problems in patient treatment. β-lactamases are encoded by extended-spectrum β-lactamase (ESBL) genes such as blaTEM and blaCTX-M. We aimed to assess the prevalence and antibiotic sensitivity of β-lactamases encoded by blaCTX-M and blaTEM in E. coli isolated from patients suffering from urinary tract infections (UTIs). Methods: Escherichia coli strains were isolated from the patients’ urine culture presented to medical diagnostic laboratories in Zabol, Iran. The agar disc-diffusion test was performed on Müller-Hinton agar to investigate the antibiotic resistance of these isolates using eight antimicrobial paper discs including gentamicin, tetracycline, co-trimoxazole, norfloxacin, cefuroxime, ampicillin, neomycin, and amoxicillin. A conventional polymerase chain reaction (PCR) was used to detect blaCTX-M and blaTEM. Results: The frequencies of resistance to cefuroxime, norfloxacin, co-trimoxazole, neomycin, amoxicillin, tetracycline, gentamicin, and ampicillin were found to be 45 (90%), 15 (30%), 33 (66%), 33 (66%), 44 (88%), 34 (68%), 4 (8%), and 50 (100%), respectively. Moreover, the prevalence of blaCTX-M was 25 (50%) while that of blaTEM was 16 (32%). Conclusion: Based on the results, gentamicin and norfloxacin can be recommended as effective antibacterials for treating UTI caused by E. coli in the study population. Moreover, the frequency of resistant genes including blaCTX-M and blaTEM was high in the isolated E. coli. Effective control systems including appropriate treatments for ESBL-producing strains are therefore required for humans and food animals. |
| first_indexed | 2024-12-19T08:26:06Z |
| format | Article |
| id | doaj.art-304e456c7a8b4385a364307bae924fae |
| institution | Directory Open Access Journal |
| issn | 2476-664X |
| language | English |
| last_indexed | 2024-12-19T08:26:06Z |
| publishDate | 2020-06-01 |
| publisher | Zabol University of Medical sciences |
| record_format | Article |
| series | International Journal of Basic Science in Medicine |
| spelling | doaj.art-304e456c7a8b4385a364307bae924fae2022-12-21T20:29:18ZengZabol University of Medical sciencesInternational Journal of Basic Science in Medicine2476-664X2020-06-0152434710.34172/ijbsm.2020.09ijbsm-13310The Frequency and Antimicrobial Resistance of blaTEM and blaCTX-M Genes in Escherichia coli Isolated From Patients With a Urinary Tract InfectionParisa Shahbazi0Mohammad Jahantigh1Saeed Salari2Salehe Danesh3Graduated Student of Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran.Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Zabol, Zabol, IranDepartment of Pathobiology, Faculty of Veterinary Medicine, University of Zabol, Zabol, IranZabol University of Medical Sciences, Zabol, IranIntroduction: The production of β-lactamase in bacteria, especially in Escherichia coli as a prevalent opportunistic bacterium, has caused many problems in patient treatment. β-lactamases are encoded by extended-spectrum β-lactamase (ESBL) genes such as blaTEM and blaCTX-M. We aimed to assess the prevalence and antibiotic sensitivity of β-lactamases encoded by blaCTX-M and blaTEM in E. coli isolated from patients suffering from urinary tract infections (UTIs). Methods: Escherichia coli strains were isolated from the patients’ urine culture presented to medical diagnostic laboratories in Zabol, Iran. The agar disc-diffusion test was performed on Müller-Hinton agar to investigate the antibiotic resistance of these isolates using eight antimicrobial paper discs including gentamicin, tetracycline, co-trimoxazole, norfloxacin, cefuroxime, ampicillin, neomycin, and amoxicillin. A conventional polymerase chain reaction (PCR) was used to detect blaCTX-M and blaTEM. Results: The frequencies of resistance to cefuroxime, norfloxacin, co-trimoxazole, neomycin, amoxicillin, tetracycline, gentamicin, and ampicillin were found to be 45 (90%), 15 (30%), 33 (66%), 33 (66%), 44 (88%), 34 (68%), 4 (8%), and 50 (100%), respectively. Moreover, the prevalence of blaCTX-M was 25 (50%) while that of blaTEM was 16 (32%). Conclusion: Based on the results, gentamicin and norfloxacin can be recommended as effective antibacterials for treating UTI caused by E. coli in the study population. Moreover, the frequency of resistant genes including blaCTX-M and blaTEM was high in the isolated E. coli. Effective control systems including appropriate treatments for ESBL-producing strains are therefore required for humans and food animals.http://ijbsm.zbmu.ac.ir/PDF/ijbsm-13310esbl e. coli prevalence antimicrobial resistance |
| spellingShingle | Parisa Shahbazi Mohammad Jahantigh Saeed Salari Salehe Danesh The Frequency and Antimicrobial Resistance of blaTEM and blaCTX-M Genes in Escherichia coli Isolated From Patients With a Urinary Tract Infection International Journal of Basic Science in Medicine esbl e. coli prevalence antimicrobial resistance |
| title | The Frequency and Antimicrobial Resistance of blaTEM and blaCTX-M Genes in Escherichia coli Isolated From Patients With a Urinary Tract Infection |
| title_full | The Frequency and Antimicrobial Resistance of blaTEM and blaCTX-M Genes in Escherichia coli Isolated From Patients With a Urinary Tract Infection |
| title_fullStr | The Frequency and Antimicrobial Resistance of blaTEM and blaCTX-M Genes in Escherichia coli Isolated From Patients With a Urinary Tract Infection |
| title_full_unstemmed | The Frequency and Antimicrobial Resistance of blaTEM and blaCTX-M Genes in Escherichia coli Isolated From Patients With a Urinary Tract Infection |
| title_short | The Frequency and Antimicrobial Resistance of blaTEM and blaCTX-M Genes in Escherichia coli Isolated From Patients With a Urinary Tract Infection |
| title_sort | frequency and antimicrobial resistance of blatem and blactx m genes in escherichia coli isolated from patients with a urinary tract infection |
| topic | esbl e. coli prevalence antimicrobial resistance |
| url | http://ijbsm.zbmu.ac.ir/PDF/ijbsm-13310 |
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