Alleviation of drought stress in maize (Zea mays L.) by using endogenous endophyte Bacillus subtilis in North West Himalayas
In the present study, we reported the isolation and molecular characterisation of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase-producing endophyte and its effect on alleviating drought stress in maize. Ten morphologically distinct indigenous plant growth-promoting rhizobacteria (PGPR) from...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-07-01
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| Series: | Acta Agriculturae Scandinavica. Section B, Soil and Plant Science |
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| Online Access: | http://dx.doi.org/10.1080/09064710.2020.1743749 |
| _version_ | 1797684387285827584 |
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| author | Gaurav Sood Rajesh Kaushal Minakshi Sharma |
| author_facet | Gaurav Sood Rajesh Kaushal Minakshi Sharma |
| author_sort | Gaurav Sood |
| collection | DOAJ |
| description | In the present study, we reported the isolation and molecular characterisation of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase-producing endophyte and its effect on alleviating drought stress in maize. Ten morphologically distinct indigenous plant growth-promoting rhizobacteria (PGPR) from maize roots and rhizosphere were screened for multiple plant growth-promoting (PGP) traits, and of ten, two strains (DHK and B1N1) possessing maximum PGP traits were evaluated at three water stress regimes in maize: (i) 80% field capacity (FC), (ii) 60% FC, and (iii) 40% FC for 45 days, starting 15 days after sowing to the maturity. Inoculation of DHK strain with maize at 80% FC induced a significant increase in plant biomass (root biomass, 86.67%; shoot biomass, 111.63%) together with decreased reactive oxygen species and increased activity of antioxidant enzymes (superoxide dismutase, 14.11 U/g fresh weight; peroxidase, 1.14 U/g fresh weight and catalase; 14.91 U/g fresh weight) compared to uninoculated control at same water regime (80% FC). The derivative strain also significantly accrued nutrients in maize and enhanced total chlorophyll and amino acid content in comparison with non-inoculated plants. 16S rDNA analysis of DHK strain revealed its lineage to Bacillus subtilis. Present investigations demonstrated the potential of the bacterial partner in alleviating drought stress in maize. |
| first_indexed | 2024-03-12T00:28:59Z |
| format | Article |
| id | doaj.art-304f4a5489b24bddbbee5f6ef469c525 |
| institution | Directory Open Access Journal |
| issn | 0906-4710 1651-1913 |
| language | English |
| last_indexed | 2024-03-12T00:28:59Z |
| publishDate | 2020-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Acta Agriculturae Scandinavica. Section B, Soil and Plant Science |
| spelling | doaj.art-304f4a5489b24bddbbee5f6ef469c5252023-09-15T10:26:25ZengTaylor & Francis GroupActa Agriculturae Scandinavica. Section B, Soil and Plant Science0906-47101651-19132020-07-0170536137010.1080/09064710.2020.17437491743749Alleviation of drought stress in maize (Zea mays L.) by using endogenous endophyte Bacillus subtilis in North West HimalayasGaurav Sood0Rajesh Kaushal1Minakshi Sharma2Department of soil science and water management, College of Forestry, Dr. Y. S. Parmar University of Horticulture and ForestryDepartment of soil science and water management, College of Forestry, Dr. Y. S. Parmar University of Horticulture and ForestryDepartment of Basic Sciences, College of Forestry, Dr. Y. S. Parmar University of Horticulture and ForestryIn the present study, we reported the isolation and molecular characterisation of 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase-producing endophyte and its effect on alleviating drought stress in maize. Ten morphologically distinct indigenous plant growth-promoting rhizobacteria (PGPR) from maize roots and rhizosphere were screened for multiple plant growth-promoting (PGP) traits, and of ten, two strains (DHK and B1N1) possessing maximum PGP traits were evaluated at three water stress regimes in maize: (i) 80% field capacity (FC), (ii) 60% FC, and (iii) 40% FC for 45 days, starting 15 days after sowing to the maturity. Inoculation of DHK strain with maize at 80% FC induced a significant increase in plant biomass (root biomass, 86.67%; shoot biomass, 111.63%) together with decreased reactive oxygen species and increased activity of antioxidant enzymes (superoxide dismutase, 14.11 U/g fresh weight; peroxidase, 1.14 U/g fresh weight and catalase; 14.91 U/g fresh weight) compared to uninoculated control at same water regime (80% FC). The derivative strain also significantly accrued nutrients in maize and enhanced total chlorophyll and amino acid content in comparison with non-inoculated plants. 16S rDNA analysis of DHK strain revealed its lineage to Bacillus subtilis. Present investigations demonstrated the potential of the bacterial partner in alleviating drought stress in maize.http://dx.doi.org/10.1080/09064710.2020.1743749droughtabiotic stressbiofertiliserspgprantioxidant enzymes |
| spellingShingle | Gaurav Sood Rajesh Kaushal Minakshi Sharma Alleviation of drought stress in maize (Zea mays L.) by using endogenous endophyte Bacillus subtilis in North West Himalayas Acta Agriculturae Scandinavica. Section B, Soil and Plant Science drought abiotic stress biofertilisers pgpr antioxidant enzymes |
| title | Alleviation of drought stress in maize (Zea mays L.) by using endogenous endophyte Bacillus subtilis in North West Himalayas |
| title_full | Alleviation of drought stress in maize (Zea mays L.) by using endogenous endophyte Bacillus subtilis in North West Himalayas |
| title_fullStr | Alleviation of drought stress in maize (Zea mays L.) by using endogenous endophyte Bacillus subtilis in North West Himalayas |
| title_full_unstemmed | Alleviation of drought stress in maize (Zea mays L.) by using endogenous endophyte Bacillus subtilis in North West Himalayas |
| title_short | Alleviation of drought stress in maize (Zea mays L.) by using endogenous endophyte Bacillus subtilis in North West Himalayas |
| title_sort | alleviation of drought stress in maize zea mays l by using endogenous endophyte bacillus subtilis in north west himalayas |
| topic | drought abiotic stress biofertilisers pgpr antioxidant enzymes |
| url | http://dx.doi.org/10.1080/09064710.2020.1743749 |
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