Comparison of vegetable oils on the uptake of lutein and zeaxanthin by ARPE-19 cells
AIM: To compare the effect of vegetable oils on the uptake of lutein and zeaxanthin by adult retinal pigment epithelial (ARPE)-19 cells in vitro. METHODS: ARPE-19 cells were cultured in Dulbecco's Modified Eagle Medium-F-12 supplemented with 10% foetal bovine serum and 1% penicillin–streptomyci...
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2023-01-01
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| Series: | International Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://ies.ijo.cn/en_publish/2023/1/20230106.pdf |
| _version_ | 1797975718244646912 |
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| author | Jeonghun Baek Chun Wai Mai Wei Meng Lim Lai Chun Wong |
| author_facet | Jeonghun Baek Chun Wai Mai Wei Meng Lim Lai Chun Wong |
| author_sort | Jeonghun Baek |
| collection | DOAJ |
| description | AIM: To compare the effect of vegetable oils on the uptake of lutein and zeaxanthin by adult retinal pigment epithelial (ARPE)-19 cells in vitro. METHODS: ARPE-19 cells were cultured in Dulbecco's Modified Eagle Medium-F-12 supplemented with 10% foetal bovine serum and 1% penicillin–streptomycin in a humidified 5% CO2 incubator maintained at 37℃. Cells were treated with 247 µmol/L lutein, 49 µmol/L zeaxanthin and 1% (v/v) of either coconut oil, corn oil, peanut oil, olive oil, sunflower oil, soybean oil, castor oil, or linseed oil for 48h. Lutein and zeaxanthin concentration in the cells were quantified by high performance liquid chromatography. RESULTS: Among the oils tested, the highest lutein and zeaxanthin uptake was observed with coconut oil while the lowest was observed with linseed oil. CONCLUSION: ARPE-19 uptake of lutein and zeaxanthin are found to be dependent on the type of oils. |
| first_indexed | 2024-04-11T04:39:56Z |
| format | Article |
| id | doaj.art-3056d3281cbd477c86da8a78aef7a945 |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-04-11T04:39:56Z |
| publishDate | 2023-01-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-3056d3281cbd477c86da8a78aef7a9452022-12-28T07:41:47ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982023-01-01161404610.18240/ijo.2023.01.0620230106Comparison of vegetable oils on the uptake of lutein and zeaxanthin by ARPE-19 cellsJeonghun Baek0Chun Wai Mai1Wei Meng Lim2Lai Chun Wong3Lai Chun Wong. Department of Pharmaceutical Chemistry, School of Pharmacy, Kuala Lumpur 57000, Malaysia. wlaichun@yahoo.comDepartment of Pharmaceutical Chemistry, School of Pharmacy, International Medical University, Kuala Lumpur 57000, MalaysiaDepartment of Pharmaceutical Technology, School of Pharmacy, International Medical University, Kuala Lumpur 57000, MalaysiaDepartment of Pharmaceutical Chemistry, School of Pharmacy, International Medical University, Kuala Lumpur 57000, MalaysiaAIM: To compare the effect of vegetable oils on the uptake of lutein and zeaxanthin by adult retinal pigment epithelial (ARPE)-19 cells in vitro. METHODS: ARPE-19 cells were cultured in Dulbecco's Modified Eagle Medium-F-12 supplemented with 10% foetal bovine serum and 1% penicillin–streptomycin in a humidified 5% CO2 incubator maintained at 37℃. Cells were treated with 247 µmol/L lutein, 49 µmol/L zeaxanthin and 1% (v/v) of either coconut oil, corn oil, peanut oil, olive oil, sunflower oil, soybean oil, castor oil, or linseed oil for 48h. Lutein and zeaxanthin concentration in the cells were quantified by high performance liquid chromatography. RESULTS: Among the oils tested, the highest lutein and zeaxanthin uptake was observed with coconut oil while the lowest was observed with linseed oil. CONCLUSION: ARPE-19 uptake of lutein and zeaxanthin are found to be dependent on the type of oils.http://ies.ijo.cn/en_publish/2023/1/20230106.pdfluteinzeaxanthinadult retinal pigment epithelial-19 cellsvegetable oil |
| spellingShingle | Jeonghun Baek Chun Wai Mai Wei Meng Lim Lai Chun Wong Comparison of vegetable oils on the uptake of lutein and zeaxanthin by ARPE-19 cells International Journal of Ophthalmology lutein zeaxanthin adult retinal pigment epithelial-19 cells vegetable oil |
| title | Comparison of vegetable oils on the uptake of lutein and zeaxanthin by ARPE-19 cells |
| title_full | Comparison of vegetable oils on the uptake of lutein and zeaxanthin by ARPE-19 cells |
| title_fullStr | Comparison of vegetable oils on the uptake of lutein and zeaxanthin by ARPE-19 cells |
| title_full_unstemmed | Comparison of vegetable oils on the uptake of lutein and zeaxanthin by ARPE-19 cells |
| title_short | Comparison of vegetable oils on the uptake of lutein and zeaxanthin by ARPE-19 cells |
| title_sort | comparison of vegetable oils on the uptake of lutein and zeaxanthin by arpe 19 cells |
| topic | lutein zeaxanthin adult retinal pigment epithelial-19 cells vegetable oil |
| url | http://ies.ijo.cn/en_publish/2023/1/20230106.pdf |
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