Increased Systemic Antioxidant Power Ameliorates the Aging-Related Reduction in Oocyte Competence in Mice
Ovarian aging is associated with elevated oxidative stress and diminished oocyte developmental competence. We aimed to determine the impact of systemic antioxidant treatment in aged mice. Female outbred CF-1 mice were aged for 9 months prior to an 8-week 45 mg <i>Euterpe oleracea</i> (aç...
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MDPI AG
2021-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/23/13019 |
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| author | Sydney L. Lane Jason C. Parks Jennifer E. Russ Shaihla A. Khan William B. Schoolcraft Ye Yuan Mandy G. Katz-Jaffe |
| author_facet | Sydney L. Lane Jason C. Parks Jennifer E. Russ Shaihla A. Khan William B. Schoolcraft Ye Yuan Mandy G. Katz-Jaffe |
| author_sort | Sydney L. Lane |
| collection | DOAJ |
| description | Ovarian aging is associated with elevated oxidative stress and diminished oocyte developmental competence. We aimed to determine the impact of systemic antioxidant treatment in aged mice. Female outbred CF-1 mice were aged for 9 months prior to an 8-week 45 mg <i>Euterpe oleracea</i> (açaí) daily supplement. The açaí treatment induced a threefold increase in serum antioxidant power (FRAP) compared to both young and aged mice (<i>p</i> < 0.0001). Compared to young mice, aged mice had fewer oocytes and reduced blastocyst development (<i>p</i> < 0.0001); açaí did not affect the oocyte numbers, but improved blastocyst formation (<i>p</i> < 0.05). Additionally, açaí alleviated the aging-related decrease in implantation potential (<i>p</i> < 0.01). The aged mice showed evidence of elevated ovarian ER stress (increased whole-ovary <i>PDIA4</i> expression, granulosa cell and oocyte <i>GRP78</i> expression, and oocyte PDIA4 protein), reduced oocyte mitochondrial quality (higher PRKN activation and mitochondrial DNA oxidative damage), and dysregulated uterine glandular epithelium. Antioxidant intervention was sufficient to lessen these effects of ovarian aging, likely in part by the upregulation of NRF2. We conclude that açaí treatment is a promising strategy to improve ER and mitochondrial function in the ovaries, thereby ameliorating the decreased oocyte competence that occurs with ovarian aging. |
| first_indexed | 2024-03-10T04:52:03Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T04:52:03Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-3059dd68ed2a46858097ef1429c5fd332023-11-23T02:31:52ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-12-0122231301910.3390/ijms222313019Increased Systemic Antioxidant Power Ameliorates the Aging-Related Reduction in Oocyte Competence in MiceSydney L. Lane0Jason C. Parks1Jennifer E. Russ2Shaihla A. Khan3William B. Schoolcraft4Ye Yuan5Mandy G. Katz-Jaffe6Colorado Center for Reproductive Medicine, Lone Tree, CO 80124, USAColorado Center for Reproductive Medicine, Lone Tree, CO 80124, USAColorado Center for Reproductive Medicine, Lone Tree, CO 80124, USAColorado Center for Reproductive Medicine, Lone Tree, CO 80124, USAColorado Center for Reproductive Medicine, Lone Tree, CO 80124, USAColorado Center for Reproductive Medicine, Lone Tree, CO 80124, USAColorado Center for Reproductive Medicine, Lone Tree, CO 80124, USAOvarian aging is associated with elevated oxidative stress and diminished oocyte developmental competence. We aimed to determine the impact of systemic antioxidant treatment in aged mice. Female outbred CF-1 mice were aged for 9 months prior to an 8-week 45 mg <i>Euterpe oleracea</i> (açaí) daily supplement. The açaí treatment induced a threefold increase in serum antioxidant power (FRAP) compared to both young and aged mice (<i>p</i> < 0.0001). Compared to young mice, aged mice had fewer oocytes and reduced blastocyst development (<i>p</i> < 0.0001); açaí did not affect the oocyte numbers, but improved blastocyst formation (<i>p</i> < 0.05). Additionally, açaí alleviated the aging-related decrease in implantation potential (<i>p</i> < 0.01). The aged mice showed evidence of elevated ovarian ER stress (increased whole-ovary <i>PDIA4</i> expression, granulosa cell and oocyte <i>GRP78</i> expression, and oocyte PDIA4 protein), reduced oocyte mitochondrial quality (higher PRKN activation and mitochondrial DNA oxidative damage), and dysregulated uterine glandular epithelium. Antioxidant intervention was sufficient to lessen these effects of ovarian aging, likely in part by the upregulation of NRF2. We conclude that açaí treatment is a promising strategy to improve ER and mitochondrial function in the ovaries, thereby ameliorating the decreased oocyte competence that occurs with ovarian aging.https://www.mdpi.com/1422-0067/22/23/13019oxidative stressantioxidantsaginginfertilityoocyte competence |
| spellingShingle | Sydney L. Lane Jason C. Parks Jennifer E. Russ Shaihla A. Khan William B. Schoolcraft Ye Yuan Mandy G. Katz-Jaffe Increased Systemic Antioxidant Power Ameliorates the Aging-Related Reduction in Oocyte Competence in Mice International Journal of Molecular Sciences oxidative stress antioxidants aging infertility oocyte competence |
| title | Increased Systemic Antioxidant Power Ameliorates the Aging-Related Reduction in Oocyte Competence in Mice |
| title_full | Increased Systemic Antioxidant Power Ameliorates the Aging-Related Reduction in Oocyte Competence in Mice |
| title_fullStr | Increased Systemic Antioxidant Power Ameliorates the Aging-Related Reduction in Oocyte Competence in Mice |
| title_full_unstemmed | Increased Systemic Antioxidant Power Ameliorates the Aging-Related Reduction in Oocyte Competence in Mice |
| title_short | Increased Systemic Antioxidant Power Ameliorates the Aging-Related Reduction in Oocyte Competence in Mice |
| title_sort | increased systemic antioxidant power ameliorates the aging related reduction in oocyte competence in mice |
| topic | oxidative stress antioxidants aging infertility oocyte competence |
| url | https://www.mdpi.com/1422-0067/22/23/13019 |
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