A Novel Recombinant Virus-Like Particles Displaying B and T Cell Epitopes of Japanese Encephalitis Virus Offers Protective Immunity in Mice and Guinea Pigs
Virus-like particles (VLPs) are non-replicative vectors for the delivery of heterologous epitopes and are considered one of the most potent inducers of cellular and humoral immune responses in mice and guinea pigs. In the present study, VLP-JEVe was constructed by the insertion of six Japanese encep...
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MDPI AG
2021-09-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/9/9/980 |
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| author | Muhammad Naveed Anwar Chunying Jiang Di Di Junjie Zhang Shuang Guo Xin Wang Muddassar Hameed Abdul Wahaab Donghua Shao Zongjie Li Ke Liu Beibei Li Yafeng Qiu Zhiyong Ma Jianchao Wei |
| author_facet | Muhammad Naveed Anwar Chunying Jiang Di Di Junjie Zhang Shuang Guo Xin Wang Muddassar Hameed Abdul Wahaab Donghua Shao Zongjie Li Ke Liu Beibei Li Yafeng Qiu Zhiyong Ma Jianchao Wei |
| author_sort | Muhammad Naveed Anwar |
| collection | DOAJ |
| description | Virus-like particles (VLPs) are non-replicative vectors for the delivery of heterologous epitopes and are considered one of the most potent inducers of cellular and humoral immune responses in mice and guinea pigs. In the present study, VLP-JEVe was constructed by the insertion of six Japanese encephalitis virus (JEV) envelope protein epitopes into different surface loop regions of PPV VP2 by the substitution of specific amino acid sequences without altering the assembly of the virus; subsequently, the protective efficacy of this VLP-JEVe was evaluated against JEV challenge in mice and guinea pigs. Mice immunized with the VLP-JEVe antigen developed high titers of neutralizing antibodies and 100% protection against lethal JEV challenge. The neutralizing and hemagglutination inhibition (HI) antibody responses were also induced in guinea pigs vaccinated with VLP-JEVe. In addition, immunization with VLP-JEVe in mice induced effective neutralizing antibodies and protective immunity against PPV (porcine parvovirus) challenge in guinea pigs. These studies suggest that VLP-JEVe produced as described here could be a potential candidate for vaccine development. |
| first_indexed | 2024-03-10T07:09:24Z |
| format | Article |
| id | doaj.art-305e49e0cc5d4a78b08d6f995159638b |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T07:09:24Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-305e49e0cc5d4a78b08d6f995159638b2023-11-22T15:34:22ZengMDPI AGVaccines2076-393X2021-09-019998010.3390/vaccines9090980A Novel Recombinant Virus-Like Particles Displaying B and T Cell Epitopes of Japanese Encephalitis Virus Offers Protective Immunity in Mice and Guinea PigsMuhammad Naveed Anwar0Chunying Jiang1Di Di2Junjie Zhang3Shuang Guo4Xin Wang5Muddassar Hameed6Abdul Wahaab7Donghua Shao8Zongjie Li9Ke Liu10Beibei Li11Yafeng Qiu12Zhiyong Ma13Jianchao Wei14Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaShanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, ChinaVirus-like particles (VLPs) are non-replicative vectors for the delivery of heterologous epitopes and are considered one of the most potent inducers of cellular and humoral immune responses in mice and guinea pigs. In the present study, VLP-JEVe was constructed by the insertion of six Japanese encephalitis virus (JEV) envelope protein epitopes into different surface loop regions of PPV VP2 by the substitution of specific amino acid sequences without altering the assembly of the virus; subsequently, the protective efficacy of this VLP-JEVe was evaluated against JEV challenge in mice and guinea pigs. Mice immunized with the VLP-JEVe antigen developed high titers of neutralizing antibodies and 100% protection against lethal JEV challenge. The neutralizing and hemagglutination inhibition (HI) antibody responses were also induced in guinea pigs vaccinated with VLP-JEVe. In addition, immunization with VLP-JEVe in mice induced effective neutralizing antibodies and protective immunity against PPV (porcine parvovirus) challenge in guinea pigs. These studies suggest that VLP-JEVe produced as described here could be a potential candidate for vaccine development.https://www.mdpi.com/2076-393X/9/9/980Japanese encephalitis virusporcine parvovirusvirus-like particleimmunogenicity |
| spellingShingle | Muhammad Naveed Anwar Chunying Jiang Di Di Junjie Zhang Shuang Guo Xin Wang Muddassar Hameed Abdul Wahaab Donghua Shao Zongjie Li Ke Liu Beibei Li Yafeng Qiu Zhiyong Ma Jianchao Wei A Novel Recombinant Virus-Like Particles Displaying B and T Cell Epitopes of Japanese Encephalitis Virus Offers Protective Immunity in Mice and Guinea Pigs Vaccines Japanese encephalitis virus porcine parvovirus virus-like particle immunogenicity |
| title | A Novel Recombinant Virus-Like Particles Displaying B and T Cell Epitopes of Japanese Encephalitis Virus Offers Protective Immunity in Mice and Guinea Pigs |
| title_full | A Novel Recombinant Virus-Like Particles Displaying B and T Cell Epitopes of Japanese Encephalitis Virus Offers Protective Immunity in Mice and Guinea Pigs |
| title_fullStr | A Novel Recombinant Virus-Like Particles Displaying B and T Cell Epitopes of Japanese Encephalitis Virus Offers Protective Immunity in Mice and Guinea Pigs |
| title_full_unstemmed | A Novel Recombinant Virus-Like Particles Displaying B and T Cell Epitopes of Japanese Encephalitis Virus Offers Protective Immunity in Mice and Guinea Pigs |
| title_short | A Novel Recombinant Virus-Like Particles Displaying B and T Cell Epitopes of Japanese Encephalitis Virus Offers Protective Immunity in Mice and Guinea Pigs |
| title_sort | novel recombinant virus like particles displaying b and t cell epitopes of japanese encephalitis virus offers protective immunity in mice and guinea pigs |
| topic | Japanese encephalitis virus porcine parvovirus virus-like particle immunogenicity |
| url | https://www.mdpi.com/2076-393X/9/9/980 |
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