Potential role of miRNAs in developmental haemostasis.
MicroRNAs (miRNAs) are an abundant class of small non-coding RNAs that are negative regulators in a crescent number of physiological and pathological processes. However, their role in haemostasis, a complex physiological process involving multitude of effectors, is just beginning to be characterized...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3052364?pdf=render |
| _version_ | 1818863317304737792 |
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| author | Raúl Teruel Javier Corral Virginia Pérez-Andreu Irene Martínez-Martínez Vicente Vicente Constantino Martínez |
| author_facet | Raúl Teruel Javier Corral Virginia Pérez-Andreu Irene Martínez-Martínez Vicente Vicente Constantino Martínez |
| author_sort | Raúl Teruel |
| collection | DOAJ |
| description | MicroRNAs (miRNAs) are an abundant class of small non-coding RNAs that are negative regulators in a crescent number of physiological and pathological processes. However, their role in haemostasis, a complex physiological process involving multitude of effectors, is just beginning to be characterized. We evaluated the changes of expression of miRNAs in livers of neonates (day one after birth) and adult mice by microarray and qRT-PCR trying to identify miRNAs that potentially may also be involved in the control of the dramatic change of hepatic haemostatic protein levels associated with this transition. Twenty one out of 41 miRNAs overexpressed in neonate mice have hepatic haemostatic mRNA as potential targets. Six of them identified by two in silico algorithms potentially bind the 3'UTR regions of F7, F9, F12, FXIIIB, PLG and SERPINC1 mRNA. Interestingly, miR-18a and miR-19b, overexpressed 5.4 and 8.2-fold respectively in neonates, have antithrombin, a key anti-coagulant with strong anti-angiogenic and anti-inflammatory roles, as a potential target. The levels of these two miRNAs inversely correlated with antithrombin mRNA levels during development (miR-19b: R = 0.81; p = 0.03; miR-18a: R = 0.91; p<0.001). These data suggest that miRNAs could be potential modulators of the haemostatic system involved in developmental haemostasis. |
| first_indexed | 2024-12-19T10:13:50Z |
| format | Article |
| id | doaj.art-30626c5141d1446ab144da07a64f0d38 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T10:13:50Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-30626c5141d1446ab144da07a64f0d382022-12-21T20:26:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0163e1764810.1371/journal.pone.0017648Potential role of miRNAs in developmental haemostasis.Raúl TeruelJavier CorralVirginia Pérez-AndreuIrene Martínez-MartínezVicente VicenteConstantino MartínezMicroRNAs (miRNAs) are an abundant class of small non-coding RNAs that are negative regulators in a crescent number of physiological and pathological processes. However, their role in haemostasis, a complex physiological process involving multitude of effectors, is just beginning to be characterized. We evaluated the changes of expression of miRNAs in livers of neonates (day one after birth) and adult mice by microarray and qRT-PCR trying to identify miRNAs that potentially may also be involved in the control of the dramatic change of hepatic haemostatic protein levels associated with this transition. Twenty one out of 41 miRNAs overexpressed in neonate mice have hepatic haemostatic mRNA as potential targets. Six of them identified by two in silico algorithms potentially bind the 3'UTR regions of F7, F9, F12, FXIIIB, PLG and SERPINC1 mRNA. Interestingly, miR-18a and miR-19b, overexpressed 5.4 and 8.2-fold respectively in neonates, have antithrombin, a key anti-coagulant with strong anti-angiogenic and anti-inflammatory roles, as a potential target. The levels of these two miRNAs inversely correlated with antithrombin mRNA levels during development (miR-19b: R = 0.81; p = 0.03; miR-18a: R = 0.91; p<0.001). These data suggest that miRNAs could be potential modulators of the haemostatic system involved in developmental haemostasis.http://europepmc.org/articles/PMC3052364?pdf=render |
| spellingShingle | Raúl Teruel Javier Corral Virginia Pérez-Andreu Irene Martínez-Martínez Vicente Vicente Constantino Martínez Potential role of miRNAs in developmental haemostasis. PLoS ONE |
| title | Potential role of miRNAs in developmental haemostasis. |
| title_full | Potential role of miRNAs in developmental haemostasis. |
| title_fullStr | Potential role of miRNAs in developmental haemostasis. |
| title_full_unstemmed | Potential role of miRNAs in developmental haemostasis. |
| title_short | Potential role of miRNAs in developmental haemostasis. |
| title_sort | potential role of mirnas in developmental haemostasis |
| url | http://europepmc.org/articles/PMC3052364?pdf=render |
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