Humic Acid Modified by Being Incorporated Into Phosphate Fertilizer Increases Its Potency in Stimulating Maize Growth and Nutrient Absorption
Humic acid-enhanced phosphate fertilizer (HAP) is widely applied in Chinese agriculture due to its high efficiency. Although the structural composition and physicochemical properties of humic acid (HA) are significantly altered during HAP production, a clear understanding of the mechanisms underlyin...
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| Format: | Article |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.885156/full |
| _version_ | 1811233406905745408 |
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| author | Jianyuan Jing Shuiqin Zhang Liang Yuan Yanting Li Chengrong Chen Bingqiang Zhao |
| author_facet | Jianyuan Jing Shuiqin Zhang Liang Yuan Yanting Li Chengrong Chen Bingqiang Zhao |
| author_sort | Jianyuan Jing |
| collection | DOAJ |
| description | Humic acid-enhanced phosphate fertilizer (HAP) is widely applied in Chinese agriculture due to its high efficiency. Although the structural composition and physicochemical properties of humic acid (HA) are significantly altered during HAP production, a clear understanding of the mechanisms underlying the biological effects of HA extracted from HAP fertilizer (PHA) on plant growth is still lacking. In the current study, we extracted PHA from HAP and assessed its effects on the dry biomass, phosphorus (P) and nitrogen (N) uptake, and P absorption rate of maize seedlings when supplied at different concentrations (2.5, 5, 10, and 25 mg C L−1) in the hydroponic culture. The root vigor, root plasma membrane H+-ATPase activity, and root nitrate reductase activity were also determined as the representative indicators of the root capacity for nutrient absorption, and used to clarify the mechanism by which PHA affects the maize growth and nutrient absorption. The results showed that the dry biomass, phosphorus uptake, nitrogen uptake, and average phosphorus absorption rates were significantly higher by 14.7–27.9%, 9.6–35.1%, 17.9–22.4%, and 22.1–31.0%, respectively, in plants treated with 2.5–5 mg C L−1 PHA compared to untreated controls. Application of 10–25 mg C L−1 raw HA resulted in similar stimulatory effects on plant growth and nutrient absorption. However, higher levels of PHA (10–25 mg C L−1) negatively impacted these indicators of plant growth. Furthermore, low PHA or high raw HA concentrations similarly improved root vigor and root plasma membrane H+-ATPase and nitrate reductase (NR) activities. These results indicate that lower concentrations of PHA can stimulate maize seedling growth and nutrient absorption to an extent that is comparable to the effect of higher concentrations of raw HA. Thus, the proportion of HA incorporated into HAP could be lower than the theoretical amount estimated through assays evaluating the biological effects of raw HA. |
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| language | English |
| last_indexed | 2024-04-12T11:19:57Z |
| publishDate | 2022-05-01 |
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| series | Frontiers in Plant Science |
| spelling | doaj.art-3062f55370c94eaf9fe1625e1d768a2e2022-12-22T03:35:24ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-05-011310.3389/fpls.2022.885156885156Humic Acid Modified by Being Incorporated Into Phosphate Fertilizer Increases Its Potency in Stimulating Maize Growth and Nutrient AbsorptionJianyuan Jing0Shuiqin Zhang1Liang Yuan2Yanting Li3Chengrong Chen4Bingqiang Zhao5Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, ChinaKey Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, ChinaSchool of Environment and Science, Australian Rivers Institute, Griffith University, Nathan, QLD, AustraliaKey Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, ChinaHumic acid-enhanced phosphate fertilizer (HAP) is widely applied in Chinese agriculture due to its high efficiency. Although the structural composition and physicochemical properties of humic acid (HA) are significantly altered during HAP production, a clear understanding of the mechanisms underlying the biological effects of HA extracted from HAP fertilizer (PHA) on plant growth is still lacking. In the current study, we extracted PHA from HAP and assessed its effects on the dry biomass, phosphorus (P) and nitrogen (N) uptake, and P absorption rate of maize seedlings when supplied at different concentrations (2.5, 5, 10, and 25 mg C L−1) in the hydroponic culture. The root vigor, root plasma membrane H+-ATPase activity, and root nitrate reductase activity were also determined as the representative indicators of the root capacity for nutrient absorption, and used to clarify the mechanism by which PHA affects the maize growth and nutrient absorption. The results showed that the dry biomass, phosphorus uptake, nitrogen uptake, and average phosphorus absorption rates were significantly higher by 14.7–27.9%, 9.6–35.1%, 17.9–22.4%, and 22.1–31.0%, respectively, in plants treated with 2.5–5 mg C L−1 PHA compared to untreated controls. Application of 10–25 mg C L−1 raw HA resulted in similar stimulatory effects on plant growth and nutrient absorption. However, higher levels of PHA (10–25 mg C L−1) negatively impacted these indicators of plant growth. Furthermore, low PHA or high raw HA concentrations similarly improved root vigor and root plasma membrane H+-ATPase and nitrate reductase (NR) activities. These results indicate that lower concentrations of PHA can stimulate maize seedling growth and nutrient absorption to an extent that is comparable to the effect of higher concentrations of raw HA. Thus, the proportion of HA incorporated into HAP could be lower than the theoretical amount estimated through assays evaluating the biological effects of raw HA.https://www.frontiersin.org/articles/10.3389/fpls.2022.885156/fullhumic acid-enhanced phosphate fertilizerhumic acidbiological activitymaizeconcentration |
| spellingShingle | Jianyuan Jing Shuiqin Zhang Liang Yuan Yanting Li Chengrong Chen Bingqiang Zhao Humic Acid Modified by Being Incorporated Into Phosphate Fertilizer Increases Its Potency in Stimulating Maize Growth and Nutrient Absorption Frontiers in Plant Science humic acid-enhanced phosphate fertilizer humic acid biological activity maize concentration |
| title | Humic Acid Modified by Being Incorporated Into Phosphate Fertilizer Increases Its Potency in Stimulating Maize Growth and Nutrient Absorption |
| title_full | Humic Acid Modified by Being Incorporated Into Phosphate Fertilizer Increases Its Potency in Stimulating Maize Growth and Nutrient Absorption |
| title_fullStr | Humic Acid Modified by Being Incorporated Into Phosphate Fertilizer Increases Its Potency in Stimulating Maize Growth and Nutrient Absorption |
| title_full_unstemmed | Humic Acid Modified by Being Incorporated Into Phosphate Fertilizer Increases Its Potency in Stimulating Maize Growth and Nutrient Absorption |
| title_short | Humic Acid Modified by Being Incorporated Into Phosphate Fertilizer Increases Its Potency in Stimulating Maize Growth and Nutrient Absorption |
| title_sort | humic acid modified by being incorporated into phosphate fertilizer increases its potency in stimulating maize growth and nutrient absorption |
| topic | humic acid-enhanced phosphate fertilizer humic acid biological activity maize concentration |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.885156/full |
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