N-acetylcysteine improves morphologic and functional aspects of ovarian grafts in rats
PURPOSE: To evaluate morphological and functional aspects of the ovarian graft in transplanted rats treated with NAC. METHODS: Female Wistar rats, virgin, 3 to 4 months old, weighing 200-250 grams were used in experiments. The rats have been kept in proper sanitary conditions, receiving food and wat...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2014-01-01
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| Series: | Acta Cirúrgica Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/pdf/acb/v29s3/0102-8650-acb-29-s3-00022.pdf |
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| author | Elivânia Marques Gomes de Amorim Luciana Lamarão Damous Maria Clara Silva Durando Márcia Viviane Alves Saraiva Marcia Kiyomi Koike Edna Frasson de Souza Montero |
| author_facet | Elivânia Marques Gomes de Amorim Luciana Lamarão Damous Maria Clara Silva Durando Márcia Viviane Alves Saraiva Marcia Kiyomi Koike Edna Frasson de Souza Montero |
| author_sort | Elivânia Marques Gomes de Amorim |
| collection | DOAJ |
| description | PURPOSE: To evaluate morphological and functional aspects of the ovarian graft in transplanted rats treated with NAC. METHODS: Female Wistar rats, virgin, 3 to 4 months old, weighing 200-250 grams were used in experiments. The rats have been kept in proper sanitary conditions, receiving food and water ad libitum. Five groups (n=10, each) were constituted: 4 groups treated subcutaneously with NAC, at doses of 150, 300, 600 and 1200 mg/kg (NAC150, NAC300, NAC600 and NAC1200, respectively), one hour of before the ovarian transplantation and control group (GTx) - treated with physiological solution and submitted to ovarian transplantation. The rats were anesthetized and submitted to autologous left ovarian transplantation, without anastomosis in retroperitoneum, and contralateral oophorectomy. During follow-up of 4 or 15 days, the estrous cycle was evaluated by vaginal smears to determine cycle regularity. At the end of 4th or 15th days, rats were re-anesthetized and blood and graft were obtained to estradiol analysis and morphological assessment. Data were analysed by One Way Analysis of Variance (ANOVA) or ANOVA on ranks complemented by Student-Newman-Keuls test. RESULTS: At 4th day, viable follicles in the graft did not altered by NAC treatments. The NAC300 and NAC600 groups showed increasing in follicle atresia (p=0.012) compared to GTx and NAC1200 group. At 15th day, 50% of GTx, NAC150, and NAC300 rats showed regular oestrous cycle; 83% of NAC600 and 100% of NAC1200 rats returned to regular cycle. NAC1200 group showed increasing in primordial follicle compared to GTx, NAC150 or NAC300 (p=0.011). NAC did not interfere in estradiol levels after 4 or 15 days of transplantation. CONCLUSION: In autologous ovarian transplantation, high dose of NAC promotes graft viability with recovery of estrous cycle. |
| first_indexed | 2024-12-24T01:00:41Z |
| format | Article |
| id | doaj.art-3067f66d85d9481aa136baf74cc2147c |
| institution | Directory Open Access Journal |
| issn | 0102-8650 |
| language | English |
| last_indexed | 2024-12-24T01:00:41Z |
| publishDate | 2014-01-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj.art-3067f66d85d9481aa136baf74cc2147c2022-12-21T17:23:22ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira0102-86502014-01-0129suppl 3222710.1590/S0102-86502014001700005N-acetylcysteine improves morphologic and functional aspects of ovarian grafts in ratsElivânia Marques Gomes de AmorimLuciana Lamarão DamousMaria Clara Silva DurandoMárcia Viviane Alves SaraivaMarcia Kiyomi KoikeEdna Frasson de Souza MonteroPURPOSE: To evaluate morphological and functional aspects of the ovarian graft in transplanted rats treated with NAC. METHODS: Female Wistar rats, virgin, 3 to 4 months old, weighing 200-250 grams were used in experiments. The rats have been kept in proper sanitary conditions, receiving food and water ad libitum. Five groups (n=10, each) were constituted: 4 groups treated subcutaneously with NAC, at doses of 150, 300, 600 and 1200 mg/kg (NAC150, NAC300, NAC600 and NAC1200, respectively), one hour of before the ovarian transplantation and control group (GTx) - treated with physiological solution and submitted to ovarian transplantation. The rats were anesthetized and submitted to autologous left ovarian transplantation, without anastomosis in retroperitoneum, and contralateral oophorectomy. During follow-up of 4 or 15 days, the estrous cycle was evaluated by vaginal smears to determine cycle regularity. At the end of 4th or 15th days, rats were re-anesthetized and blood and graft were obtained to estradiol analysis and morphological assessment. Data were analysed by One Way Analysis of Variance (ANOVA) or ANOVA on ranks complemented by Student-Newman-Keuls test. RESULTS: At 4th day, viable follicles in the graft did not altered by NAC treatments. The NAC300 and NAC600 groups showed increasing in follicle atresia (p=0.012) compared to GTx and NAC1200 group. At 15th day, 50% of GTx, NAC150, and NAC300 rats showed regular oestrous cycle; 83% of NAC600 and 100% of NAC1200 rats returned to regular cycle. NAC1200 group showed increasing in primordial follicle compared to GTx, NAC150 or NAC300 (p=0.011). NAC did not interfere in estradiol levels after 4 or 15 days of transplantation. CONCLUSION: In autologous ovarian transplantation, high dose of NAC promotes graft viability with recovery of estrous cycle.http://www.scielo.br/pdf/acb/v29s3/0102-8650-acb-29-s3-00022.pdfTransplantationAutologousAcetylcysteineHistologyEstrogenOvariumRats |
| spellingShingle | Elivânia Marques Gomes de Amorim Luciana Lamarão Damous Maria Clara Silva Durando Márcia Viviane Alves Saraiva Marcia Kiyomi Koike Edna Frasson de Souza Montero N-acetylcysteine improves morphologic and functional aspects of ovarian grafts in rats Acta Cirúrgica Brasileira Transplantation Autologous Acetylcysteine Histology Estrogen Ovarium Rats |
| title | N-acetylcysteine improves morphologic and functional aspects of ovarian grafts in rats |
| title_full | N-acetylcysteine improves morphologic and functional aspects of ovarian grafts in rats |
| title_fullStr | N-acetylcysteine improves morphologic and functional aspects of ovarian grafts in rats |
| title_full_unstemmed | N-acetylcysteine improves morphologic and functional aspects of ovarian grafts in rats |
| title_short | N-acetylcysteine improves morphologic and functional aspects of ovarian grafts in rats |
| title_sort | n acetylcysteine improves morphologic and functional aspects of ovarian grafts in rats |
| topic | Transplantation Autologous Acetylcysteine Histology Estrogen Ovarium Rats |
| url | http://www.scielo.br/pdf/acb/v29s3/0102-8650-acb-29-s3-00022.pdf |
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