Transcriptomic Profiling the Effects of Airway Exposure of Zinc Oxide and Silver Nanoparticles in Mouse Lungs
Consumers and manufacturers are exposed to nanosized zinc oxide (nZnO) and silver particles (nAg) via airways, but their biological effects are still not fully elucidated. To understand the immune effects, we exposed mice to 2, 10, or 50 μg of nZnO or nAg by oropharyngeal aspiration and analyzed the...
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MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/6/5183 |
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| author | Lan Zhao Shuyuan Wang Marit Ilves Sanna Lehtonen Leena Saikko Hani El-Nezami Harri Alenius Piia Karisola |
| author_facet | Lan Zhao Shuyuan Wang Marit Ilves Sanna Lehtonen Leena Saikko Hani El-Nezami Harri Alenius Piia Karisola |
| author_sort | Lan Zhao |
| collection | DOAJ |
| description | Consumers and manufacturers are exposed to nanosized zinc oxide (nZnO) and silver particles (nAg) via airways, but their biological effects are still not fully elucidated. To understand the immune effects, we exposed mice to 2, 10, or 50 μg of nZnO or nAg by oropharyngeal aspiration and analyzed the global gene expression profiles and immunopathological changes in the lungs after 1, 7, or 28 days. Our results show that the kinetics of responses varied in the lungs. Exposure to nZnO resulted in the highest accumulation of F4/80- and CD3-positive cells, and the largest number of differentially expressed genes (DEGs) were identified after day 1, while exposure to nAg caused peak responses at day 7. Additionally, nZnO mainly activated the innate immune responses leading to acute inflammation, whereas the nAg activated both innate and adaptive immune pathways, with long-lasting effects. This kinetic-profiling study provides an important data source to understand the cellular and molecular processes underlying nZnO- and nAg-induced transcriptomic changes, which lead to the characterization of the corresponding biological and toxicological effects of nZnO and nAg in the lungs. These findings could improve science-based hazard and risk assessment and the development of safe applications of engineered nanomaterials (ENMs), e.g., in biomedical applications. |
| first_indexed | 2024-03-11T06:26:51Z |
| format | Article |
| id | doaj.art-306b81a74a5e48ebb24f5891be21e0cc |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T06:26:51Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-306b81a74a5e48ebb24f5891be21e0cc2023-11-17T11:30:16ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01246518310.3390/ijms24065183Transcriptomic Profiling the Effects of Airway Exposure of Zinc Oxide and Silver Nanoparticles in Mouse LungsLan Zhao0Shuyuan Wang1Marit Ilves2Sanna Lehtonen3Leena Saikko4Hani El-Nezami5Harri Alenius6Piia Karisola7Human Microbiome Research (HUMI), Medical Faculty, University of Helsinki, 00014 Helsinki, FinlandHuman Microbiome Research (HUMI), Medical Faculty, University of Helsinki, 00014 Helsinki, FinlandHuman Microbiome Research (HUMI), Medical Faculty, University of Helsinki, 00014 Helsinki, FinlandDepartment of Pathology, Medical Faculty, University of Helsinki, 00014 Helsinki, FinlandDepartment of Pathology, Medical Faculty, University of Helsinki, 00014 Helsinki, FinlandSchool of Biological Sciences, University of Hong Kong, Hong Kong, ChinaHuman Microbiome Research (HUMI), Medical Faculty, University of Helsinki, 00014 Helsinki, FinlandHuman Microbiome Research (HUMI), Medical Faculty, University of Helsinki, 00014 Helsinki, FinlandConsumers and manufacturers are exposed to nanosized zinc oxide (nZnO) and silver particles (nAg) via airways, but their biological effects are still not fully elucidated. To understand the immune effects, we exposed mice to 2, 10, or 50 μg of nZnO or nAg by oropharyngeal aspiration and analyzed the global gene expression profiles and immunopathological changes in the lungs after 1, 7, or 28 days. Our results show that the kinetics of responses varied in the lungs. Exposure to nZnO resulted in the highest accumulation of F4/80- and CD3-positive cells, and the largest number of differentially expressed genes (DEGs) were identified after day 1, while exposure to nAg caused peak responses at day 7. Additionally, nZnO mainly activated the innate immune responses leading to acute inflammation, whereas the nAg activated both innate and adaptive immune pathways, with long-lasting effects. This kinetic-profiling study provides an important data source to understand the cellular and molecular processes underlying nZnO- and nAg-induced transcriptomic changes, which lead to the characterization of the corresponding biological and toxicological effects of nZnO and nAg in the lungs. These findings could improve science-based hazard and risk assessment and the development of safe applications of engineered nanomaterials (ENMs), e.g., in biomedical applications.https://www.mdpi.com/1422-0067/24/6/5183animal modelimmune effectslungsilver nanoparticlestranscriptomicszinc oxide nanoparticles |
| spellingShingle | Lan Zhao Shuyuan Wang Marit Ilves Sanna Lehtonen Leena Saikko Hani El-Nezami Harri Alenius Piia Karisola Transcriptomic Profiling the Effects of Airway Exposure of Zinc Oxide and Silver Nanoparticles in Mouse Lungs International Journal of Molecular Sciences animal model immune effects lung silver nanoparticles transcriptomics zinc oxide nanoparticles |
| title | Transcriptomic Profiling the Effects of Airway Exposure of Zinc Oxide and Silver Nanoparticles in Mouse Lungs |
| title_full | Transcriptomic Profiling the Effects of Airway Exposure of Zinc Oxide and Silver Nanoparticles in Mouse Lungs |
| title_fullStr | Transcriptomic Profiling the Effects of Airway Exposure of Zinc Oxide and Silver Nanoparticles in Mouse Lungs |
| title_full_unstemmed | Transcriptomic Profiling the Effects of Airway Exposure of Zinc Oxide and Silver Nanoparticles in Mouse Lungs |
| title_short | Transcriptomic Profiling the Effects of Airway Exposure of Zinc Oxide and Silver Nanoparticles in Mouse Lungs |
| title_sort | transcriptomic profiling the effects of airway exposure of zinc oxide and silver nanoparticles in mouse lungs |
| topic | animal model immune effects lung silver nanoparticles transcriptomics zinc oxide nanoparticles |
| url | https://www.mdpi.com/1422-0067/24/6/5183 |
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