Amaryllidaceae Alkaloids from <i>Clivia miniata</i> (Lindl.) Bosse (Amaryllidaceae): Isolation, Structural Elucidation, and Biological Activity
<i>Clivia miniata</i> (Amaryllidaceae) is an herbaceous evergreen flowering plant that is endemic to South Africa and Swaziland and belongs to one of the top-10 traded medicinal plants in informal medicine markets in South Africa. The species has been reported as the most important compo...
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MDPI AG
2022-11-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/22/3034 |
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| author | Marcela Šafratová Jana Křoustková Negar Maafi Daniela Suchánková Rudolf Vrabec Jakub Chlebek Jiří Kuneš Lubomír Opletal Franz Bucar Lucie Cahlíková |
| author_facet | Marcela Šafratová Jana Křoustková Negar Maafi Daniela Suchánková Rudolf Vrabec Jakub Chlebek Jiří Kuneš Lubomír Opletal Franz Bucar Lucie Cahlíková |
| author_sort | Marcela Šafratová |
| collection | DOAJ |
| description | <i>Clivia miniata</i> (Amaryllidaceae) is an herbaceous evergreen flowering plant that is endemic to South Africa and Swaziland and belongs to one of the top-10 traded medicinal plants in informal medicine markets in South Africa. The species has been reported as the most important component of a traditional healer’s pallet of healing plants. Eighteen known Amaryllidaceae alkaloids (AAs) of various structural types, and one undescribed alkaloid of homolycorine-type, named clivimine B (<b>3</b>), were isolated from <i>Clivia miniata</i>. The chemical structures of the isolated alkaloids were elucidated by a combination of MS, HRMS, 1D and 2D NMR techniques and by comparison with literature data. Compounds isolated in a sufficient quantity, and not tested previously, were evaluated for their in vitro acetylcholinesterase (AChE; E.C. 3.1.1.7) and butyrylcholinesterase (BuChE; E.C. 3.1.1.8) inhibition activities. |
| first_indexed | 2024-03-09T18:03:47Z |
| format | Article |
| id | doaj.art-306d8ecd42a849309b99a0173e98bfaa |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T18:03:47Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-306d8ecd42a849309b99a0173e98bfaa2023-11-24T09:38:33ZengMDPI AGPlants2223-77472022-11-011122303410.3390/plants11223034Amaryllidaceae Alkaloids from <i>Clivia miniata</i> (Lindl.) Bosse (Amaryllidaceae): Isolation, Structural Elucidation, and Biological ActivityMarcela Šafratová0Jana Křoustková1Negar Maafi2Daniela Suchánková3Rudolf Vrabec4Jakub Chlebek5Jiří Kuneš6Lubomír Opletal7Franz Bucar8Lucie Cahlíková9Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmacy, Charles University, Heyrovskeho 1203, 500 05 Hradec Kralove, Czech RepublicDepartment of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmacy, Charles University, Heyrovskeho 1203, 500 05 Hradec Kralove, Czech RepublicDepartment of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmacy, Charles University, Heyrovskeho 1203, 500 05 Hradec Kralove, Czech RepublicDepartment of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmacy, Charles University, Heyrovskeho 1203, 500 05 Hradec Kralove, Czech RepublicDepartment of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmacy, Charles University, Heyrovskeho 1203, 500 05 Hradec Kralove, Czech RepublicDepartment of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmacy, Charles University, Heyrovskeho 1203, 500 05 Hradec Kralove, Czech RepublicDepartment of Bioorganic and Organic Chemistry, Faculty of Pharmacy, Charles University, Heyrovskeho 1203, 500 05 Hradec Kralove, Czech RepublicDepartment of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmacy, Charles University, Heyrovskeho 1203, 500 05 Hradec Kralove, Czech RepublicInstitute of Pharmaceutical Sciences, University of Graz, Beethovenstraße 8, 8010 Graz, AustriaDepartment of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmacy, Charles University, Heyrovskeho 1203, 500 05 Hradec Kralove, Czech Republic<i>Clivia miniata</i> (Amaryllidaceae) is an herbaceous evergreen flowering plant that is endemic to South Africa and Swaziland and belongs to one of the top-10 traded medicinal plants in informal medicine markets in South Africa. The species has been reported as the most important component of a traditional healer’s pallet of healing plants. Eighteen known Amaryllidaceae alkaloids (AAs) of various structural types, and one undescribed alkaloid of homolycorine-type, named clivimine B (<b>3</b>), were isolated from <i>Clivia miniata</i>. The chemical structures of the isolated alkaloids were elucidated by a combination of MS, HRMS, 1D and 2D NMR techniques and by comparison with literature data. Compounds isolated in a sufficient quantity, and not tested previously, were evaluated for their in vitro acetylcholinesterase (AChE; E.C. 3.1.1.7) and butyrylcholinesterase (BuChE; E.C. 3.1.1.8) inhibition activities.https://www.mdpi.com/2223-7747/11/22/3034acetylcholinesteraseAmaryllidaceaebutyrylcholinesterase<i>Clivia miniata</i>clivimine B |
| spellingShingle | Marcela Šafratová Jana Křoustková Negar Maafi Daniela Suchánková Rudolf Vrabec Jakub Chlebek Jiří Kuneš Lubomír Opletal Franz Bucar Lucie Cahlíková Amaryllidaceae Alkaloids from <i>Clivia miniata</i> (Lindl.) Bosse (Amaryllidaceae): Isolation, Structural Elucidation, and Biological Activity Plants acetylcholinesterase Amaryllidaceae butyrylcholinesterase <i>Clivia miniata</i> clivimine B |
| title | Amaryllidaceae Alkaloids from <i>Clivia miniata</i> (Lindl.) Bosse (Amaryllidaceae): Isolation, Structural Elucidation, and Biological Activity |
| title_full | Amaryllidaceae Alkaloids from <i>Clivia miniata</i> (Lindl.) Bosse (Amaryllidaceae): Isolation, Structural Elucidation, and Biological Activity |
| title_fullStr | Amaryllidaceae Alkaloids from <i>Clivia miniata</i> (Lindl.) Bosse (Amaryllidaceae): Isolation, Structural Elucidation, and Biological Activity |
| title_full_unstemmed | Amaryllidaceae Alkaloids from <i>Clivia miniata</i> (Lindl.) Bosse (Amaryllidaceae): Isolation, Structural Elucidation, and Biological Activity |
| title_short | Amaryllidaceae Alkaloids from <i>Clivia miniata</i> (Lindl.) Bosse (Amaryllidaceae): Isolation, Structural Elucidation, and Biological Activity |
| title_sort | amaryllidaceae alkaloids from i clivia miniata i lindl bosse amaryllidaceae isolation structural elucidation and biological activity |
| topic | acetylcholinesterase Amaryllidaceae butyrylcholinesterase <i>Clivia miniata</i> clivimine B |
| url | https://www.mdpi.com/2223-7747/11/22/3034 |
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