Are There Any Differences in the Neural and Extracellular Matrix Proteins Density Between Children and Adults with Intrinsic Ureteropelvic Junction Obstruction?
Objective: To compare changes in connective, neural and muscle tissues, and extracellular matrix in child and adult patients so that we can create a more objective view on the timing of surgery. Materials and Methods: Twenty-six patients, who were operated for ureteropelvic junction (UPJ) obstruct...
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| Format: | Article |
| Language: | English |
| Published: |
Galenos Yayinevi
2017-12-01
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| Series: | Journal of Urological Surgery |
| Subjects: | |
| Online Access: | http://jurolsurgery.org/archives/archive-detail/article-preview/are-there-any-differences-in-the-neural-and-extrac/16567 |
| _version_ | 1797910221792739328 |
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| author | Bahadır Şahin Cevdet Kaya Farhad Talibzade Yılören Tanıdır Feriha Ercan Elif Kervancıoğlu Demirci Çağrı Akın Şekerci Cem Akbal Ferruh Şimşek |
| author_facet | Bahadır Şahin Cevdet Kaya Farhad Talibzade Yılören Tanıdır Feriha Ercan Elif Kervancıoğlu Demirci Çağrı Akın Şekerci Cem Akbal Ferruh Şimşek |
| author_sort | Bahadır Şahin |
| collection | DOAJ |
| description | Objective: To compare changes in connective, neural and muscle tissues, and extracellular matrix in child and adult patients so that we can create
a more objective view on the timing of surgery.
Materials and Methods: Twenty-six patients, who were operated for ureteropelvic junction (UPJ) obstruction in our clinic between September
2014 and May 2016, were included in the study. For the evaluation of connective tissue, Masson’s trichrome staining was used. Muscle, extracellular
matrix elements and neural tissue were evaluated with immunohistochemistry using alpha-smooth muscle actin (α-SMA), Tenascin C and S100,
respectively. Microscopically, the tissues were scored according to their staining density (0: No staining; 1: Minor; 2: Moderate, 3: Dense).
Results: There were 12 children and 14 adults in the study groups. The initial evaluation showed no statistically significant difference between
studied tissue types with respect to staining density for all parameters (Masson: p=0.414, α-SMA: p=0.204, Tenascin-C: p=0.264, S100: p=0.534).
There was no statistically significant correlation between staining density and renal function percentage of the affected kidney (Masson: r=0.454,
p=0.051 - α-SMA: r=-0.323, p=0.177 - Tenascin-C: r=0.290, p=0.229 - S100: r=-0.080, p=0.744).
Conclusion: Our preliminary study showed some structural changes between adult and child patients but there is no statistically significant
difference between the groups with respect to staining density scores. These results state that although UPJ obstruction is an ongoing process, there
is no correlation between the histological deterioration degree of the UPJ segment and the loss on renal function for both children and adults. |
| first_indexed | 2024-04-10T11:20:55Z |
| format | Article |
| id | doaj.art-306f19154e584c1ca98c0d45857215b1 |
| institution | Directory Open Access Journal |
| issn | 2148-9580 |
| language | English |
| last_indexed | 2024-04-10T11:20:55Z |
| publishDate | 2017-12-01 |
| publisher | Galenos Yayinevi |
| record_format | Article |
| series | Journal of Urological Surgery |
| spelling | doaj.art-306f19154e584c1ca98c0d45857215b12023-02-15T16:18:39ZengGalenos YayineviJournal of Urological Surgery2148-95802017-12-014417718210.4274/jus.1655Are There Any Differences in the Neural and Extracellular Matrix Proteins Density Between Children and Adults with Intrinsic Ureteropelvic Junction Obstruction?Bahadır Şahin0Cevdet Kaya1Farhad Talibzade2Yılören Tanıdır3Feriha Ercan4Elif Kervancıoğlu Demirci5Çağrı Akın Şekerci6Cem Akbal7Ferruh Şimşek8Marmara University Faculty of Medicine, Department of Urology, İstanbul, TurkiyeMarmara University Faculty of Medicine, Department of Urology, İstanbul, TurkiyeMarmara University Faculty of Medicine, Department of Urology, İstanbul, TurkiyeMarmara University Faculty of Medicine, Department of Urology, İstanbul, TurkiyeMarmara University Faculty of Medicine, Department of Histology-Embryology, İstanbul, TurkiyeMarmara University Faculty of Medicine, Department of Histology-Embryology, İstanbul, TurkiyeMarmara University Faculty of Medicine, Department of Urology, İstanbul, TurkiyeMarmara University Faculty of Medicine, Department of Urology, İstanbul, TurkiyeMarmara University Faculty of Medicine, Department of Urology, İstanbul, TurkiyeObjective: To compare changes in connective, neural and muscle tissues, and extracellular matrix in child and adult patients so that we can create a more objective view on the timing of surgery. Materials and Methods: Twenty-six patients, who were operated for ureteropelvic junction (UPJ) obstruction in our clinic between September 2014 and May 2016, were included in the study. For the evaluation of connective tissue, Masson’s trichrome staining was used. Muscle, extracellular matrix elements and neural tissue were evaluated with immunohistochemistry using alpha-smooth muscle actin (α-SMA), Tenascin C and S100, respectively. Microscopically, the tissues were scored according to their staining density (0: No staining; 1: Minor; 2: Moderate, 3: Dense). Results: There were 12 children and 14 adults in the study groups. The initial evaluation showed no statistically significant difference between studied tissue types with respect to staining density for all parameters (Masson: p=0.414, α-SMA: p=0.204, Tenascin-C: p=0.264, S100: p=0.534). There was no statistically significant correlation between staining density and renal function percentage of the affected kidney (Masson: r=0.454, p=0.051 - α-SMA: r=-0.323, p=0.177 - Tenascin-C: r=0.290, p=0.229 - S100: r=-0.080, p=0.744). Conclusion: Our preliminary study showed some structural changes between adult and child patients but there is no statistically significant difference between the groups with respect to staining density scores. These results state that although UPJ obstruction is an ongoing process, there is no correlation between the histological deterioration degree of the UPJ segment and the loss on renal function for both children and adults.http://jurolsurgery.org/archives/archive-detail/article-preview/are-there-any-differences-in-the-neural-and-extrac/16567Extracellular matriximmunohistochemistrymuscle tissueconnective tissueneural tissueureteropelvic junction |
| spellingShingle | Bahadır Şahin Cevdet Kaya Farhad Talibzade Yılören Tanıdır Feriha Ercan Elif Kervancıoğlu Demirci Çağrı Akın Şekerci Cem Akbal Ferruh Şimşek Are There Any Differences in the Neural and Extracellular Matrix Proteins Density Between Children and Adults with Intrinsic Ureteropelvic Junction Obstruction? Journal of Urological Surgery Extracellular matrix immunohistochemistry muscle tissue connective tissue neural tissue ureteropelvic junction |
| title | Are There Any Differences in the Neural and Extracellular Matrix Proteins Density Between Children and Adults with Intrinsic Ureteropelvic Junction Obstruction? |
| title_full | Are There Any Differences in the Neural and Extracellular Matrix Proteins Density Between Children and Adults with Intrinsic Ureteropelvic Junction Obstruction? |
| title_fullStr | Are There Any Differences in the Neural and Extracellular Matrix Proteins Density Between Children and Adults with Intrinsic Ureteropelvic Junction Obstruction? |
| title_full_unstemmed | Are There Any Differences in the Neural and Extracellular Matrix Proteins Density Between Children and Adults with Intrinsic Ureteropelvic Junction Obstruction? |
| title_short | Are There Any Differences in the Neural and Extracellular Matrix Proteins Density Between Children and Adults with Intrinsic Ureteropelvic Junction Obstruction? |
| title_sort | are there any differences in the neural and extracellular matrix proteins density between children and adults with intrinsic ureteropelvic junction obstruction |
| topic | Extracellular matrix immunohistochemistry muscle tissue connective tissue neural tissue ureteropelvic junction |
| url | http://jurolsurgery.org/archives/archive-detail/article-preview/are-there-any-differences-in-the-neural-and-extrac/16567 |
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