Functional Characterization of Laccase Isozyme (PoLcc1) from the Edible Mushroom <i>Pleurotus ostreatus</i> Involved in Lignin Degradation in Cotton Straw
Fungal laccases play important roles in the degradation of lignocellulose. In this study, the laccase producing cotton straw medium for <i>Pleurotus ostreatus</i> was optimized by single-factor and orthogonal experiments, and to investigate the role of <i>Lacc1</i> gene, one...
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/21/13545 |
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| author | Guoqing Li Yahui Wang Peilei Zhu Guiyun Zhao Caiyu Liu Hongyuan Zhao |
| author_facet | Guoqing Li Yahui Wang Peilei Zhu Guiyun Zhao Caiyu Liu Hongyuan Zhao |
| author_sort | Guoqing Li |
| collection | DOAJ |
| description | Fungal laccases play important roles in the degradation of lignocellulose. In this study, the laccase producing cotton straw medium for <i>Pleurotus ostreatus</i> was optimized by single-factor and orthogonal experiments, and to investigate the role of <i>Lacc1</i> gene, one of the laccase-encoding genes, in the degradation of cotton straw lignin, an overexpression strain of <i>Lacc1</i> gene was constructed, which was analyzed for the characteristics of lignin degradation. The results demonstrated that the culture conditions with the highest lignin degradation efficiency of the <i>P. ostreatus</i> were the cotton straw particle size of 0.75 mm, a solid–liquid ratio of 1:3 and containing 0.25 g/L of Tween in the medium, as well as an incubation temperature of 26 °C. Two overexpression strains (OE L1-1 and OE L1-4) of <i>Lacc1</i> gene were obtained, and the gene expression increased 12.08- and 33.04-fold, respectively. The results of <sup>1</sup>H-NMR and FTIR analyses of significant changes in lignin structure revealed that <i>Lacc1</i> gene accelerated the degradation of lignin G-units and involved in the cleavage of β-O-4 linkages and the demethylation of lignin units. These findings will help to improve the efficiency of biodelignification and expand our understanding of its mechanism. |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T18:59:25Z |
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| spelling | doaj.art-306fb71611484a6e9798baf225b5dc872023-11-24T05:09:44ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123211354510.3390/ijms232113545Functional Characterization of Laccase Isozyme (PoLcc1) from the Edible Mushroom <i>Pleurotus ostreatus</i> Involved in Lignin Degradation in Cotton StrawGuoqing Li0Yahui Wang1Peilei Zhu2Guiyun Zhao3Caiyu Liu4Hongyuan Zhao5State Key Laboratory of Horticultural Crop Germplasm Resources Creation and Utilization of Ministry of Agriculture and Rural Affairs, Institute of Horticulture Research, Anhui Academy of Agricultural Sciences, Hefei 230031, ChinaState Key Laboratory of Horticultural Crop Germplasm Resources Creation and Utilization of Ministry of Agriculture and Rural Affairs, Institute of Horticulture Research, Anhui Academy of Agricultural Sciences, Hefei 230031, ChinaState Key Laboratory of Horticultural Crop Germplasm Resources Creation and Utilization of Ministry of Agriculture and Rural Affairs, Institute of Horticulture Research, Anhui Academy of Agricultural Sciences, Hefei 230031, ChinaState Key Laboratory of Horticultural Crop Germplasm Resources Creation and Utilization of Ministry of Agriculture and Rural Affairs, Institute of Horticulture Research, Anhui Academy of Agricultural Sciences, Hefei 230031, ChinaState Key Laboratory of Horticultural Crop Germplasm Resources Creation and Utilization of Ministry of Agriculture and Rural Affairs, Institute of Horticulture Research, Anhui Academy of Agricultural Sciences, Hefei 230031, ChinaState Key Laboratory of Horticultural Crop Germplasm Resources Creation and Utilization of Ministry of Agriculture and Rural Affairs, Institute of Horticulture Research, Anhui Academy of Agricultural Sciences, Hefei 230031, ChinaFungal laccases play important roles in the degradation of lignocellulose. In this study, the laccase producing cotton straw medium for <i>Pleurotus ostreatus</i> was optimized by single-factor and orthogonal experiments, and to investigate the role of <i>Lacc1</i> gene, one of the laccase-encoding genes, in the degradation of cotton straw lignin, an overexpression strain of <i>Lacc1</i> gene was constructed, which was analyzed for the characteristics of lignin degradation. The results demonstrated that the culture conditions with the highest lignin degradation efficiency of the <i>P. ostreatus</i> were the cotton straw particle size of 0.75 mm, a solid–liquid ratio of 1:3 and containing 0.25 g/L of Tween in the medium, as well as an incubation temperature of 26 °C. Two overexpression strains (OE L1-1 and OE L1-4) of <i>Lacc1</i> gene were obtained, and the gene expression increased 12.08- and 33.04-fold, respectively. The results of <sup>1</sup>H-NMR and FTIR analyses of significant changes in lignin structure revealed that <i>Lacc1</i> gene accelerated the degradation of lignin G-units and involved in the cleavage of β-O-4 linkages and the demethylation of lignin units. These findings will help to improve the efficiency of biodelignification and expand our understanding of its mechanism.https://www.mdpi.com/1422-0067/23/21/13545<i>Pleurotus ostreatus</i>laccase<i>Lacc1</i>cotton stalklignin degradationfunctional groups |
| spellingShingle | Guoqing Li Yahui Wang Peilei Zhu Guiyun Zhao Caiyu Liu Hongyuan Zhao Functional Characterization of Laccase Isozyme (PoLcc1) from the Edible Mushroom <i>Pleurotus ostreatus</i> Involved in Lignin Degradation in Cotton Straw International Journal of Molecular Sciences <i>Pleurotus ostreatus</i> laccase <i>Lacc1</i> cotton stalk lignin degradation functional groups |
| title | Functional Characterization of Laccase Isozyme (PoLcc1) from the Edible Mushroom <i>Pleurotus ostreatus</i> Involved in Lignin Degradation in Cotton Straw |
| title_full | Functional Characterization of Laccase Isozyme (PoLcc1) from the Edible Mushroom <i>Pleurotus ostreatus</i> Involved in Lignin Degradation in Cotton Straw |
| title_fullStr | Functional Characterization of Laccase Isozyme (PoLcc1) from the Edible Mushroom <i>Pleurotus ostreatus</i> Involved in Lignin Degradation in Cotton Straw |
| title_full_unstemmed | Functional Characterization of Laccase Isozyme (PoLcc1) from the Edible Mushroom <i>Pleurotus ostreatus</i> Involved in Lignin Degradation in Cotton Straw |
| title_short | Functional Characterization of Laccase Isozyme (PoLcc1) from the Edible Mushroom <i>Pleurotus ostreatus</i> Involved in Lignin Degradation in Cotton Straw |
| title_sort | functional characterization of laccase isozyme polcc1 from the edible mushroom i pleurotus ostreatus i involved in lignin degradation in cotton straw |
| topic | <i>Pleurotus ostreatus</i> laccase <i>Lacc1</i> cotton stalk lignin degradation functional groups |
| url | https://www.mdpi.com/1422-0067/23/21/13545 |
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