Dietary arginine silicate inositol complex increased bone healing: histologic and histomorphometric study
Ferhan Yaman,1 Izzet Acikan,1 Serkan Dundar,2 Sercan Simsek,3 Mehmet Gul,4 İbrahim Hanifi Ozercan,3 James Komorowski,5 Kazim Sahin6 1Department of Oral-Maxillofacial Surgery, Faculty of Dentistry, Dicle University, Diyarbakir, Turkey; 2Department of Periodontology, Faculty of Dentistry, Firat Unive...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2016-06-01
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| Series: | Drug Design, Development and Therapy |
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| Online Access: | https://www.dovepress.com/dietary-arginine-silicate-inositol-complex-increased-bone-healing-hist-peer-reviewed-article-DDDT |
| _version_ | 1818217788287746048 |
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| author | Yaman F Acikan I Dundar S Simsek S Gul M Ozercan İH Komorowski J Sahin K |
| author_facet | Yaman F Acikan I Dundar S Simsek S Gul M Ozercan İH Komorowski J Sahin K |
| author_sort | Yaman F |
| collection | DOAJ |
| description | Ferhan Yaman,1 Izzet Acikan,1 Serkan Dundar,2 Sercan Simsek,3 Mehmet Gul,4 İbrahim Hanifi Ozercan,3 James Komorowski,5 Kazim Sahin6 1Department of Oral-Maxillofacial Surgery, Faculty of Dentistry, Dicle University, Diyarbakir, Turkey; 2Department of Periodontology, Faculty of Dentistry, Firat University, Elazig, Turkey; 3Department of Pathology, Faculty of Medicine, Firat University, Elazig, Turkey; 4Department of Periodontology, Faculty of Dentistry, Dicle University, Diyarbakir, Turkey; 5Nutrition 21, LLC, Purchase, NY, USA; 6Department of Animal Nutrition, Faculty of Veterinary Medicine, Firat University, Elazig, Turkey Background: Arginine silicate inositol complex (ASI; arginine 49.5%, silicon 8.2%, and inositol 25%) is a novel material that is a bioavailable source of silicon and arginine. ASI offers potential benefits for vascular and bone health. Objective: The aim of this study was to evaluate the potential effects of ASI complex on bone healing of critical-sized defects in rats. Methods: The rats were randomly assigned to two groups of 21 rats each. The control group was fed a standard diet for 12 weeks; after the first 8 weeks, a calvarial critical-sized defect was created, and the rats were sacrificed 7, 14, and 28 days later. The ASI group was fed a diet containing 1.81 g/kg of ASI for 12 weeks; after the first 8 weeks, a calvarial critical-sized defect was created, and the rats were sacrificed 7, 14, and 28 days later. The calvarial bones of all the rats were then harvested for evaluation. Results: Osteoblasts and osteoclasts were detected at higher levels in the ASI group compared with the control group at days 7, 14, and 28 of the calvarial defect (P<0.05). New bone formation was detected at higher levels in the ASI group compared with the controls at day 28 (P<0.05). However, new bone formation was not detected at days 7 and 14 in both the groups (P>0.05). Conclusion: ASI supplementation significantly improved bone tissue healing in rats with critical-sized defects. This study demonstrated that ASI can enhance bone repair and has potential as a therapeutic regimen in humans. Keywords: arginine silicate inositol, bone healing, osteoblast, osteoclast, critical-sized defect |
| first_indexed | 2024-12-12T07:13:26Z |
| format | Article |
| id | doaj.art-30715e04b4c544f0adf1e35e2e4885be |
| institution | Directory Open Access Journal |
| issn | 1177-8881 |
| language | English |
| last_indexed | 2024-12-12T07:13:26Z |
| publishDate | 2016-06-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Drug Design, Development and Therapy |
| spelling | doaj.art-30715e04b4c544f0adf1e35e2e4885be2022-12-22T00:33:35ZengDove Medical PressDrug Design, Development and Therapy1177-88812016-06-012016Issue 12081208627624Dietary arginine silicate inositol complex increased bone healing: histologic and histomorphometric studyYaman FAcikan IDundar SSimsek SGul MOzercan İHKomorowski JSahin KFerhan Yaman,1 Izzet Acikan,1 Serkan Dundar,2 Sercan Simsek,3 Mehmet Gul,4 İbrahim Hanifi Ozercan,3 James Komorowski,5 Kazim Sahin6 1Department of Oral-Maxillofacial Surgery, Faculty of Dentistry, Dicle University, Diyarbakir, Turkey; 2Department of Periodontology, Faculty of Dentistry, Firat University, Elazig, Turkey; 3Department of Pathology, Faculty of Medicine, Firat University, Elazig, Turkey; 4Department of Periodontology, Faculty of Dentistry, Dicle University, Diyarbakir, Turkey; 5Nutrition 21, LLC, Purchase, NY, USA; 6Department of Animal Nutrition, Faculty of Veterinary Medicine, Firat University, Elazig, Turkey Background: Arginine silicate inositol complex (ASI; arginine 49.5%, silicon 8.2%, and inositol 25%) is a novel material that is a bioavailable source of silicon and arginine. ASI offers potential benefits for vascular and bone health. Objective: The aim of this study was to evaluate the potential effects of ASI complex on bone healing of critical-sized defects in rats. Methods: The rats were randomly assigned to two groups of 21 rats each. The control group was fed a standard diet for 12 weeks; after the first 8 weeks, a calvarial critical-sized defect was created, and the rats were sacrificed 7, 14, and 28 days later. The ASI group was fed a diet containing 1.81 g/kg of ASI for 12 weeks; after the first 8 weeks, a calvarial critical-sized defect was created, and the rats were sacrificed 7, 14, and 28 days later. The calvarial bones of all the rats were then harvested for evaluation. Results: Osteoblasts and osteoclasts were detected at higher levels in the ASI group compared with the control group at days 7, 14, and 28 of the calvarial defect (P<0.05). New bone formation was detected at higher levels in the ASI group compared with the controls at day 28 (P<0.05). However, new bone formation was not detected at days 7 and 14 in both the groups (P>0.05). Conclusion: ASI supplementation significantly improved bone tissue healing in rats with critical-sized defects. This study demonstrated that ASI can enhance bone repair and has potential as a therapeutic regimen in humans. Keywords: arginine silicate inositol, bone healing, osteoblast, osteoclast, critical-sized defecthttps://www.dovepress.com/dietary-arginine-silicate-inositol-complex-increased-bone-healing-hist-peer-reviewed-article-DDDTArginine slicate inositolbone healingosteoblastosteoclastcritical sized defect |
| spellingShingle | Yaman F Acikan I Dundar S Simsek S Gul M Ozercan İH Komorowski J Sahin K Dietary arginine silicate inositol complex increased bone healing: histologic and histomorphometric study Drug Design, Development and Therapy Arginine slicate inositol bone healing osteoblast osteoclast critical sized defect |
| title | Dietary arginine silicate inositol complex increased bone healing: histologic and histomorphometric study |
| title_full | Dietary arginine silicate inositol complex increased bone healing: histologic and histomorphometric study |
| title_fullStr | Dietary arginine silicate inositol complex increased bone healing: histologic and histomorphometric study |
| title_full_unstemmed | Dietary arginine silicate inositol complex increased bone healing: histologic and histomorphometric study |
| title_short | Dietary arginine silicate inositol complex increased bone healing: histologic and histomorphometric study |
| title_sort | dietary arginine silicate inositol complex increased bone healing histologic and histomorphometric study |
| topic | Arginine slicate inositol bone healing osteoblast osteoclast critical sized defect |
| url | https://www.dovepress.com/dietary-arginine-silicate-inositol-complex-increased-bone-healing-hist-peer-reviewed-article-DDDT |
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