EVALUATION OF SUITABILITY OF METHODS FOR TESTING BLOOD PROTEIN HYDROLYSATES FOR THEIR BIOLOGICAL ACTIVITY AND TOXICITY
The use of high quality nutrient media comprising protein hydrolysates as the main components for maintaining their nutrient value is a critical prerequisite for appropriate fnal product preparation in FMD vaccine biotechnology. The incoming control of raw materials, in particular testing blood prot...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Da Vinci Media
2019-04-01
|
| Series: | Ветеринария сегодня |
| Subjects: | |
| Online Access: | https://veterinary.arriah.ru/jour/article/view/402 |
| _version_ | 1797224860523429888 |
|---|---|
| author | M. N. Guseva M. I. Doronin M. A. Shevchenko D. V. Mikhalishin A. A. Shishkova |
| author_facet | M. N. Guseva M. I. Doronin M. A. Shevchenko D. V. Mikhalishin A. A. Shishkova |
| author_sort | M. N. Guseva |
| collection | DOAJ |
| description | The use of high quality nutrient media comprising protein hydrolysates as the main components for maintaining their nutrient value is a critical prerequisite for appropriate fnal product preparation in FMD vaccine biotechnology. The incoming control of raw materials, in particular testing blood protein hydrolysates of different batches for their quality, is of considerable importance. Currently, there are different methods for the determination of hydrolysate efcacy using cell cultures and unicellular organisms as test systems. We used suspended baby hamster kidney (ВНК-21) cell line as a model for testing blood protein hydrolysates in the raw materials intended for FMD vaccine production for their biological activity and toxicity. The validation of the proposed method was carried out to confrm the reliability of the test results obtained. The following main validation characteristics were determined through testing 560 blood protein hydrolysate samples: trueness, specifcity, sensitivity, as well as precision under repeatability and reproducibility conditions. The validation results for the proposed method demonstrated high reliability (Fisher’s criterion – 0.021; ≤0.050) and full compliance with acceptance criteria. |
| first_indexed | 2024-03-09T02:49:02Z |
| format | Article |
| id | doaj.art-30718f4a50bb4d1cb2b08bc3c5cb041a |
| institution | Directory Open Access Journal |
| issn | 2304-196X 2658-6959 |
| language | English |
| last_indexed | 2024-04-24T13:59:50Z |
| publishDate | 2019-04-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj.art-30718f4a50bb4d1cb2b08bc3c5cb041a2024-04-03T12:48:49ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592019-04-0101515710.29326/2304-196X-2019-1-28-51-57398EVALUATION OF SUITABILITY OF METHODS FOR TESTING BLOOD PROTEIN HYDROLYSATES FOR THEIR BIOLOGICAL ACTIVITY AND TOXICITYM. N. Guseva0M. I. Doronin1M. A. Shevchenko2D. V. Mikhalishin3A. A. Shishkova4FGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirThe use of high quality nutrient media comprising protein hydrolysates as the main components for maintaining their nutrient value is a critical prerequisite for appropriate fnal product preparation in FMD vaccine biotechnology. The incoming control of raw materials, in particular testing blood protein hydrolysates of different batches for their quality, is of considerable importance. Currently, there are different methods for the determination of hydrolysate efcacy using cell cultures and unicellular organisms as test systems. We used suspended baby hamster kidney (ВНК-21) cell line as a model for testing blood protein hydrolysates in the raw materials intended for FMD vaccine production for their biological activity and toxicity. The validation of the proposed method was carried out to confrm the reliability of the test results obtained. The following main validation characteristics were determined through testing 560 blood protein hydrolysate samples: trueness, specifcity, sensitivity, as well as precision under repeatability and reproducibility conditions. The validation results for the proposed method demonstrated high reliability (Fisher’s criterion – 0.021; ≤0.050) and full compliance with acceptance criteria.https://veterinary.arriah.ru/jour/article/view/402blood protein hydrolysatebiological activitytoxicityspecifcitytest-system sensitivitytrueness of methodsprecision |
| spellingShingle | M. N. Guseva M. I. Doronin M. A. Shevchenko D. V. Mikhalishin A. A. Shishkova EVALUATION OF SUITABILITY OF METHODS FOR TESTING BLOOD PROTEIN HYDROLYSATES FOR THEIR BIOLOGICAL ACTIVITY AND TOXICITY Ветеринария сегодня blood protein hydrolysate biological activity toxicity specifcity test-system sensitivity trueness of methods precision |
| title | EVALUATION OF SUITABILITY OF METHODS FOR TESTING BLOOD PROTEIN HYDROLYSATES FOR THEIR BIOLOGICAL ACTIVITY AND TOXICITY |
| title_full | EVALUATION OF SUITABILITY OF METHODS FOR TESTING BLOOD PROTEIN HYDROLYSATES FOR THEIR BIOLOGICAL ACTIVITY AND TOXICITY |
| title_fullStr | EVALUATION OF SUITABILITY OF METHODS FOR TESTING BLOOD PROTEIN HYDROLYSATES FOR THEIR BIOLOGICAL ACTIVITY AND TOXICITY |
| title_full_unstemmed | EVALUATION OF SUITABILITY OF METHODS FOR TESTING BLOOD PROTEIN HYDROLYSATES FOR THEIR BIOLOGICAL ACTIVITY AND TOXICITY |
| title_short | EVALUATION OF SUITABILITY OF METHODS FOR TESTING BLOOD PROTEIN HYDROLYSATES FOR THEIR BIOLOGICAL ACTIVITY AND TOXICITY |
| title_sort | evaluation of suitability of methods for testing blood protein hydrolysates for their biological activity and toxicity |
| topic | blood protein hydrolysate biological activity toxicity specifcity test-system sensitivity trueness of methods precision |
| url | https://veterinary.arriah.ru/jour/article/view/402 |
| work_keys_str_mv | AT mnguseva evaluationofsuitabilityofmethodsfortestingbloodproteinhydrolysatesfortheirbiologicalactivityandtoxicity AT midoronin evaluationofsuitabilityofmethodsfortestingbloodproteinhydrolysatesfortheirbiologicalactivityandtoxicity AT mashevchenko evaluationofsuitabilityofmethodsfortestingbloodproteinhydrolysatesfortheirbiologicalactivityandtoxicity AT dvmikhalishin evaluationofsuitabilityofmethodsfortestingbloodproteinhydrolysatesfortheirbiologicalactivityandtoxicity AT aashishkova evaluationofsuitabilityofmethodsfortestingbloodproteinhydrolysatesfortheirbiologicalactivityandtoxicity |