Therapeutic Effects of Eugenol in Polycystic Ovarian Rats Induced by Estradiol Valerate: A Histopathological and A Biochemical Study
Background: Polycystic ovary syndrome (PCOS) is a common type of endocrinopathy in women which is accompaniedby androgens elevation, insulin resistance, and metabolic dysfunction. Eugenol is a phenolic component of clove oil thathas an antioxidant, anti-inflammatory, and anti-diabetic activity. The...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Royan Institute (ACECR), Tehran
2022-07-01
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| Series: | International Journal of Fertility and Sterility |
| Subjects: | |
| Online Access: | https://www.ijfs.ir/article_247970_faebc7a04993d97408bd9625e6f3bc19.pdf |
| Summary: | Background: Polycystic ovary syndrome (PCOS) is a common type of endocrinopathy in women which is accompaniedby androgens elevation, insulin resistance, and metabolic dysfunction. Eugenol is a phenolic component of clove oil thathas an antioxidant, anti-inflammatory, and anti-diabetic activity. The present study aimed to evaluate the therapeutic effectsof eugenol on the PCOS models of rats.Materials and Methods: In this experimental study, thirty adults female Wistar rats weighing between 180 and 200g were used. Estradiol valerate-induced PCOS rats (4 mg/rat) were treated with eugenol (12 and 24 mg/kg) for 28days. The effects of eugenol were studied on levels of glucose, lipid profile, liver enzymes, reproductive hormones,oxidative stress, and the expression of cyclooxygenase-2 (Cox-2) and peroxisome proliferator-activated receptor alpha(Ppar-α) genes, using biochemical analysis of blood and histopathological evaluation of ovaries.Results: Estradiol valerate-induced PCOS resulted in the formation of cystic follicles in the ovaries, hyperinsulinemia,hyperglycemia, hyperlipidemia, hyperandrogenism, and anovulation. It altered the Cox-2 and Ppar-α gene expressionand increased oxidative stress and activities of liver enzymes. Eugenol treatment improved the PCOS-associatedendocrine and metabolic disorder and histopathological alterations, mostly through antioxidant, anti-diabetic,anti-hyperlipidemic, and anti-androgenic properties. It showed beneficial effects on serum glucose, serum insulin,fat profile, reproductive hormones, liver activity, oxidative stress, expression of Cox-2 and Ppar-α genes, as well asrestoration of normal ovulation in the PCOS animals.Conclusion: Eugenol could represent a promising natural product to prevent PCOS or reduce its symptoms. |
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| ISSN: | 2008-076X 2008-0778 |