Highly Water-Dispersed and Stable Deinoxanthin Nanocapsule for Effective Antioxidant and Anti-Inflammatory Activity
Sohyeon Yu,1,2 Sangwoo Kim,1,3 Jisu Kim,1,3 Ji-Woong Kim,4 Su Young Kim,4 Bongjun Yeom,2 Hyungjun Kim,5 Won II Choi,1 Daekyung Sung1 1Center for Bio-Healthcare Materials, Bio-Convergence Materials R&D Division, Korea Institute of Ceramic Engineering and Technology, Cheongju, 28160, Republic of K...
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Dove Medical Press
2023-08-01
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| Series: | International Journal of Nanomedicine |
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| Online Access: | https://www.dovepress.com/highly-water-dispersed-and-stable-deinoxanthin-nanocapsule-for-effecti-peer-reviewed-fulltext-article-IJN |
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| author | Yu S Kim S Kim J Kim JW Kim SY Yeom B Kim H Choi WI Sung D |
| author_facet | Yu S Kim S Kim J Kim JW Kim SY Yeom B Kim H Choi WI Sung D |
| author_sort | Yu S |
| collection | DOAJ |
| description | Sohyeon Yu,1,2 Sangwoo Kim,1,3 Jisu Kim,1,3 Ji-Woong Kim,4 Su Young Kim,4 Bongjun Yeom,2 Hyungjun Kim,5 Won II Choi,1 Daekyung Sung1 1Center for Bio-Healthcare Materials, Bio-Convergence Materials R&D Division, Korea Institute of Ceramic Engineering and Technology, Cheongju, 28160, Republic of Korea; 2Department of Chemical Engineering, Hanyang University, Seoul, 04763, Republic of Korea; 3Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, 03722, Republic of Korea; 4Materials Science Research Institute, LABIO Co., Ltd, Seoul, 08501, Republic of Korea; 5Department of Chemistry and Bioscience, Kumoh National Institute of Technology, Gyeongbuk, 39177, Republic of KoreaCorrespondence: Won II Choi; Daekyung Sung, Center for Bio-Healthcare Materials, Bio-Convergence Materials R&D Division, Korea Institute of Ceramic Engineering and Technology, Cheongju, 28160, Republic of Korea, Tel +82-43-913-1513 ; +82-43-913-1511, Fax +82-43-913-1597, Email choi830509@kicet.re.kr; dksung@kicet.re.krIntroduction: Deinoxanthin (DX), a carotenoid, has excellent antioxidant and anti-inflammatory properties. However, owing to its lipophilicity, it is unfavorably dispersed in water and has low stability, limiting its application in cosmetics, food, and pharmaceuticals. Therefore, it is necessary to study nanoparticles to increase the loading capacity and stability of DX.Methods: In this study, DX-loaded nanocapsules (DX@NCs) were prepared by nanoprecipitation by loading DX into nanocapsules. The size, polydispersity index, surface charge, and morphology of DX@NCs were confirmed through dynamic light scattering and transmission electron microscopy. The loading content and loading efficiency of DX in DX@NCs were analyzed using high-performance liquid chromatography. The antioxidant activity of DX@NCs was evaluated by DPPH assay and in vitro ROS. The biocompatibility of DX@NCs was evaluated using an in vitro MTT assay. In vitro NO analysis was performed to determine the effective anti-inflammatory efficacy of DX@NCs.Results: DX@NCs exhibited increased stability and antioxidant efficacy owing to the improved water solubility of DX. The in situ and in vitro antioxidant activity of DX@NCs was higher than that of unloaded DX. In addition, it showed a strong anti-inflammatory effect by regulating the NO level in an in vitro cell model.Conclusion: This study presents a nanocarrier to improve the water-soluble dispersion and stability of DX. These results demonstrate that DX@NC is a carrier with excellent stability and has a high potential for use in cosmetic and pharmaceutical applications owing to its antioxidant and anti-inflammatory effects.Keywords: deinoxanthin, nanoprecipitation, reactive oxygen species scavenging, antioxidant activity, anti-inflammation |
| first_indexed | 2024-03-12T15:23:32Z |
| format | Article |
| id | doaj.art-30775a6da7954df8aac9f2dc884b298a |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-03-12T15:23:32Z |
| publishDate | 2023-08-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-30775a6da7954df8aac9f2dc884b298a2023-08-10T19:04:44ZengDove Medical PressInternational Journal of Nanomedicine1178-20132023-08-01Volume 184555456585798Highly Water-Dispersed and Stable Deinoxanthin Nanocapsule for Effective Antioxidant and Anti-Inflammatory ActivityYu SKim SKim JKim JWKim SYYeom BKim HChoi WISung DSohyeon Yu,1,2 Sangwoo Kim,1,3 Jisu Kim,1,3 Ji-Woong Kim,4 Su Young Kim,4 Bongjun Yeom,2 Hyungjun Kim,5 Won II Choi,1 Daekyung Sung1 1Center for Bio-Healthcare Materials, Bio-Convergence Materials R&D Division, Korea Institute of Ceramic Engineering and Technology, Cheongju, 28160, Republic of Korea; 2Department of Chemical Engineering, Hanyang University, Seoul, 04763, Republic of Korea; 3Department of Chemical and Biomolecular Engineering, Yonsei University, Seoul, 03722, Republic of Korea; 4Materials Science Research Institute, LABIO Co., Ltd, Seoul, 08501, Republic of Korea; 5Department of Chemistry and Bioscience, Kumoh National Institute of Technology, Gyeongbuk, 39177, Republic of KoreaCorrespondence: Won II Choi; Daekyung Sung, Center for Bio-Healthcare Materials, Bio-Convergence Materials R&D Division, Korea Institute of Ceramic Engineering and Technology, Cheongju, 28160, Republic of Korea, Tel +82-43-913-1513 ; +82-43-913-1511, Fax +82-43-913-1597, Email choi830509@kicet.re.kr; dksung@kicet.re.krIntroduction: Deinoxanthin (DX), a carotenoid, has excellent antioxidant and anti-inflammatory properties. However, owing to its lipophilicity, it is unfavorably dispersed in water and has low stability, limiting its application in cosmetics, food, and pharmaceuticals. Therefore, it is necessary to study nanoparticles to increase the loading capacity and stability of DX.Methods: In this study, DX-loaded nanocapsules (DX@NCs) were prepared by nanoprecipitation by loading DX into nanocapsules. The size, polydispersity index, surface charge, and morphology of DX@NCs were confirmed through dynamic light scattering and transmission electron microscopy. The loading content and loading efficiency of DX in DX@NCs were analyzed using high-performance liquid chromatography. The antioxidant activity of DX@NCs was evaluated by DPPH assay and in vitro ROS. The biocompatibility of DX@NCs was evaluated using an in vitro MTT assay. In vitro NO analysis was performed to determine the effective anti-inflammatory efficacy of DX@NCs.Results: DX@NCs exhibited increased stability and antioxidant efficacy owing to the improved water solubility of DX. The in situ and in vitro antioxidant activity of DX@NCs was higher than that of unloaded DX. In addition, it showed a strong anti-inflammatory effect by regulating the NO level in an in vitro cell model.Conclusion: This study presents a nanocarrier to improve the water-soluble dispersion and stability of DX. These results demonstrate that DX@NC is a carrier with excellent stability and has a high potential for use in cosmetic and pharmaceutical applications owing to its antioxidant and anti-inflammatory effects.Keywords: deinoxanthin, nanoprecipitation, reactive oxygen species scavenging, antioxidant activity, anti-inflammationhttps://www.dovepress.com/highly-water-dispersed-and-stable-deinoxanthin-nanocapsule-for-effecti-peer-reviewed-fulltext-article-IJNdeinoxanthinnanoprecipitationreactive oxygen species scavengingantioxidant activityanti-inflammation |
| spellingShingle | Yu S Kim S Kim J Kim JW Kim SY Yeom B Kim H Choi WI Sung D Highly Water-Dispersed and Stable Deinoxanthin Nanocapsule for Effective Antioxidant and Anti-Inflammatory Activity International Journal of Nanomedicine deinoxanthin nanoprecipitation reactive oxygen species scavenging antioxidant activity anti-inflammation |
| title | Highly Water-Dispersed and Stable Deinoxanthin Nanocapsule for Effective Antioxidant and Anti-Inflammatory Activity |
| title_full | Highly Water-Dispersed and Stable Deinoxanthin Nanocapsule for Effective Antioxidant and Anti-Inflammatory Activity |
| title_fullStr | Highly Water-Dispersed and Stable Deinoxanthin Nanocapsule for Effective Antioxidant and Anti-Inflammatory Activity |
| title_full_unstemmed | Highly Water-Dispersed and Stable Deinoxanthin Nanocapsule for Effective Antioxidant and Anti-Inflammatory Activity |
| title_short | Highly Water-Dispersed and Stable Deinoxanthin Nanocapsule for Effective Antioxidant and Anti-Inflammatory Activity |
| title_sort | highly water dispersed and stable deinoxanthin nanocapsule for effective antioxidant and anti inflammatory activity |
| topic | deinoxanthin nanoprecipitation reactive oxygen species scavenging antioxidant activity anti-inflammation |
| url | https://www.dovepress.com/highly-water-dispersed-and-stable-deinoxanthin-nanocapsule-for-effecti-peer-reviewed-fulltext-article-IJN |
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