Optimization of immobilized Lactobacillus pentosus cell fermentation for lactic acid production
Abstract Parametric optimization is an effective way in fermentation process to improve product yield and productivity in order to save time, space and financial resources. In this study, Box–Behnken design was applied to optimize the conditions for lactic acid production by immobilized Lactobacillu...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-03-01
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| Series: | Bioresources and Bioprocessing |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40643-020-00305-x |
| _version_ | 1819080705932525568 |
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| author | Jianfei Wang Jiaqi Huang Hannah Laffend Shaoming Jiang Jing Zhang Yuchen Ning Mudannan Fang Shijie Liu |
| author_facet | Jianfei Wang Jiaqi Huang Hannah Laffend Shaoming Jiang Jing Zhang Yuchen Ning Mudannan Fang Shijie Liu |
| author_sort | Jianfei Wang |
| collection | DOAJ |
| description | Abstract Parametric optimization is an effective way in fermentation process to improve product yield and productivity in order to save time, space and financial resources. In this study, Box–Behnken design was applied to optimize the conditions for lactic acid production by immobilized Lactobacillus pentosus ATCC 8041 cell fermentation. Two quadratic models and response surface methodology were performed to illustrate the effect of each parameters and their interactions on the lactic acid yield and glucose consumption rate in immobilized L. pentosus ATCC 8041 cell fermentation. The maximum lactic acid yield was obtained as 0.938 ± 0.003 g/g glucose with a productivity of 2.213 ± 0.008 g/(L × h) under the optimized conditions of 2.0 mm bead diameter, 5.60 pH, 115.3 g/L initial glucose concentration, and 398.2 mg biomass (CDW) in 100 mL hydrogel. The analysis of variance indicated that the quadratic model was significant and could be used to scale up the fermentation process. |
| first_indexed | 2024-12-21T19:49:08Z |
| format | Article |
| id | doaj.art-3078596a0bcb4f0b851afdd245d6d89a |
| institution | Directory Open Access Journal |
| issn | 2197-4365 |
| language | English |
| last_indexed | 2024-12-21T19:49:08Z |
| publishDate | 2020-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Bioresources and Bioprocessing |
| spelling | doaj.art-3078596a0bcb4f0b851afdd245d6d89a2022-12-21T18:52:15ZengSpringerOpenBioresources and Bioprocessing2197-43652020-03-017111410.1186/s40643-020-00305-xOptimization of immobilized Lactobacillus pentosus cell fermentation for lactic acid productionJianfei Wang0Jiaqi Huang1Hannah Laffend2Shaoming Jiang3Jing Zhang4Yuchen Ning5Mudannan Fang6Shijie Liu7Department of Paper and Bioprocess Engineering, SUNY College of Environmental Science and ForestryDepartment of Paper and Bioprocess Engineering, SUNY College of Environmental Science and ForestryDepartment of Paper and Bioprocess Engineering, SUNY College of Environmental Science and ForestryDepartment of Paper and Bioprocess Engineering, SUNY College of Environmental Science and ForestryDepartment of Paper and Bioprocess Engineering, SUNY College of Environmental Science and ForestryDepartment of Paper and Bioprocess Engineering, SUNY College of Environmental Science and ForestryDepartment of Paper and Bioprocess Engineering, SUNY College of Environmental Science and ForestryDepartment of Paper and Bioprocess Engineering, SUNY College of Environmental Science and ForestryAbstract Parametric optimization is an effective way in fermentation process to improve product yield and productivity in order to save time, space and financial resources. In this study, Box–Behnken design was applied to optimize the conditions for lactic acid production by immobilized Lactobacillus pentosus ATCC 8041 cell fermentation. Two quadratic models and response surface methodology were performed to illustrate the effect of each parameters and their interactions on the lactic acid yield and glucose consumption rate in immobilized L. pentosus ATCC 8041 cell fermentation. The maximum lactic acid yield was obtained as 0.938 ± 0.003 g/g glucose with a productivity of 2.213 ± 0.008 g/(L × h) under the optimized conditions of 2.0 mm bead diameter, 5.60 pH, 115.3 g/L initial glucose concentration, and 398.2 mg biomass (CDW) in 100 mL hydrogel. The analysis of variance indicated that the quadratic model was significant and could be used to scale up the fermentation process.http://link.springer.com/article/10.1186/s40643-020-00305-xLactobacillus pentosusImmobilizationLactic acidOptimization |
| spellingShingle | Jianfei Wang Jiaqi Huang Hannah Laffend Shaoming Jiang Jing Zhang Yuchen Ning Mudannan Fang Shijie Liu Optimization of immobilized Lactobacillus pentosus cell fermentation for lactic acid production Bioresources and Bioprocessing Lactobacillus pentosus Immobilization Lactic acid Optimization |
| title | Optimization of immobilized Lactobacillus pentosus cell fermentation for lactic acid production |
| title_full | Optimization of immobilized Lactobacillus pentosus cell fermentation for lactic acid production |
| title_fullStr | Optimization of immobilized Lactobacillus pentosus cell fermentation for lactic acid production |
| title_full_unstemmed | Optimization of immobilized Lactobacillus pentosus cell fermentation for lactic acid production |
| title_short | Optimization of immobilized Lactobacillus pentosus cell fermentation for lactic acid production |
| title_sort | optimization of immobilized lactobacillus pentosus cell fermentation for lactic acid production |
| topic | Lactobacillus pentosus Immobilization Lactic acid Optimization |
| url | http://link.springer.com/article/10.1186/s40643-020-00305-x |
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