Evaluation of acoustic changes in and the healing outcomes of rat eardrums with pars tensa and pars flaccida perforations
Abstract Objectives To systematically explore the differences in acoustic changes and healing outcomes of tympanic membranes (TMs) with pars flaccida perforation (PFP) and pars tensa perforation (PTP). Methods We created PFPs and PTPs of various sizes in Sprague–Dawley rats, and evaluated TM umbo ve...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-06-01
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| Series: | Laryngoscope Investigative Otolaryngology |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/lio2.797 |
| _version_ | 1818204216325308416 |
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| author | Yaoqian Liu Cuiping Wu Tingting Chen Qiyue Shen Yuanping Xiong Zhengnong Chen Chunyan Li |
| author_facet | Yaoqian Liu Cuiping Wu Tingting Chen Qiyue Shen Yuanping Xiong Zhengnong Chen Chunyan Li |
| author_sort | Yaoqian Liu |
| collection | DOAJ |
| description | Abstract Objectives To systematically explore the differences in acoustic changes and healing outcomes of tympanic membranes (TMs) with pars flaccida perforation (PFP) and pars tensa perforation (PTP). Methods We created PFPs and PTPs of various sizes in Sprague–Dawley rats, and evaluated TM umbo velocity and hearing function using laser Doppler vibrometry and auditory brainstem response (ABR) measurement before and immediately after perforation. Two weeks later, hearing was reevaluated and TMs were investigated by immunohistochemical staining. Results Small PFPs and PTPs did not significantly affect umbo velocity and hearing function. Large PFPs increased umbo velocity loss at low frequency (1.5 kHz) and elevated ABR thresholds within 1–2 kHz. Large PTP caused significant velocity loss at low frequencies from 1.5 to 3.5 kHz and threshold elevations at full frequencies (1–2 kHz). Two weeks after the perforation, the hearing function of rats with healed PFPs recovered completely. However, high‐frequency hearing loss (16–32 kHz) persisted in rats with healed PTPs. Morphological staining revealed that no increase in the thickness and obvious increase in collagen I level of regenerated par flaccida; regenerated pars tensa exhibited obvious increase in thickness and increased collagen I, while the collagen II regeneration was limited with discontinuous and disordered structure in regenerated pars tensa. Conclusion The hearing loss caused by large PFP limits at low frequencies while large PTP can lead to hearing loss at wide range frequencies. PFP and PTP have different functional outcomes after spontaneous healing, which is determined by the discrepant structure reconstruction and collagen regeneration. |
| first_indexed | 2024-12-12T03:37:43Z |
| format | Article |
| id | doaj.art-30795de59c84418b9e1ac1b8edc01079 |
| institution | Directory Open Access Journal |
| issn | 2378-8038 |
| language | English |
| last_indexed | 2024-12-12T03:37:43Z |
| publishDate | 2022-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Laryngoscope Investigative Otolaryngology |
| spelling | doaj.art-30795de59c84418b9e1ac1b8edc010792022-12-22T00:39:45ZengWileyLaryngoscope Investigative Otolaryngology2378-80382022-06-017381682410.1002/lio2.797Evaluation of acoustic changes in and the healing outcomes of rat eardrums with pars tensa and pars flaccida perforationsYaoqian Liu0Cuiping Wu1Tingting Chen2Qiyue Shen3Yuanping Xiong4Zhengnong Chen5Chunyan Li6Otolaryngology Research Institute Shanghai Jiao Tong University Affiliated Sixth People's Hospital Shanghai ChinaOtolaryngology Research Institute Shanghai Jiao Tong University Affiliated Sixth People's Hospital Shanghai ChinaDepartment of Hearing and Language Rehabilitation Zhejiang Chinese Medicine University Hangzhou ChinaDepartment of Hearing and Language Rehabilitation Shanghai University of Traditional Chinese Medicine Shanghai ChinaDepartment of Otolaryngology Head and Neck Surgery First Affiliated Hospital of Nanchang University Nanchang ChinaOtolaryngology Research Institute Shanghai Jiao Tong University Affiliated Sixth People's Hospital Shanghai ChinaOtolaryngology Research Institute Shanghai Jiao Tong University Affiliated Sixth People's Hospital Shanghai ChinaAbstract Objectives To systematically explore the differences in acoustic changes and healing outcomes of tympanic membranes (TMs) with pars flaccida perforation (PFP) and pars tensa perforation (PTP). Methods We created PFPs and PTPs of various sizes in Sprague–Dawley rats, and evaluated TM umbo velocity and hearing function using laser Doppler vibrometry and auditory brainstem response (ABR) measurement before and immediately after perforation. Two weeks later, hearing was reevaluated and TMs were investigated by immunohistochemical staining. Results Small PFPs and PTPs did not significantly affect umbo velocity and hearing function. Large PFPs increased umbo velocity loss at low frequency (1.5 kHz) and elevated ABR thresholds within 1–2 kHz. Large PTP caused significant velocity loss at low frequencies from 1.5 to 3.5 kHz and threshold elevations at full frequencies (1–2 kHz). Two weeks after the perforation, the hearing function of rats with healed PFPs recovered completely. However, high‐frequency hearing loss (16–32 kHz) persisted in rats with healed PTPs. Morphological staining revealed that no increase in the thickness and obvious increase in collagen I level of regenerated par flaccida; regenerated pars tensa exhibited obvious increase in thickness and increased collagen I, while the collagen II regeneration was limited with discontinuous and disordered structure in regenerated pars tensa. Conclusion The hearing loss caused by large PFP limits at low frequencies while large PTP can lead to hearing loss at wide range frequencies. PFP and PTP have different functional outcomes after spontaneous healing, which is determined by the discrepant structure reconstruction and collagen regeneration.https://doi.org/10.1002/lio2.797auditory brainstem responsecollagenpars flaccida perforationpars tensa perforationumbo velocity |
| spellingShingle | Yaoqian Liu Cuiping Wu Tingting Chen Qiyue Shen Yuanping Xiong Zhengnong Chen Chunyan Li Evaluation of acoustic changes in and the healing outcomes of rat eardrums with pars tensa and pars flaccida perforations Laryngoscope Investigative Otolaryngology auditory brainstem response collagen pars flaccida perforation pars tensa perforation umbo velocity |
| title | Evaluation of acoustic changes in and the healing outcomes of rat eardrums with pars tensa and pars flaccida perforations |
| title_full | Evaluation of acoustic changes in and the healing outcomes of rat eardrums with pars tensa and pars flaccida perforations |
| title_fullStr | Evaluation of acoustic changes in and the healing outcomes of rat eardrums with pars tensa and pars flaccida perforations |
| title_full_unstemmed | Evaluation of acoustic changes in and the healing outcomes of rat eardrums with pars tensa and pars flaccida perforations |
| title_short | Evaluation of acoustic changes in and the healing outcomes of rat eardrums with pars tensa and pars flaccida perforations |
| title_sort | evaluation of acoustic changes in and the healing outcomes of rat eardrums with pars tensa and pars flaccida perforations |
| topic | auditory brainstem response collagen pars flaccida perforation pars tensa perforation umbo velocity |
| url | https://doi.org/10.1002/lio2.797 |
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