Metabolite and transcriptome analyses reveal the effects of salinity stress on the biosynthesis of proanthocyanidins and anthocyanins in grape suspension cells
Proanthocyanidins (PAs) and anthocyanins are flavonoids that contribute to the quality and health benefits of grapes and wine. Salinity affects their biosynthesis, but the underlying mechanism is still unclear. We studied the effects of NaCl stress on PA and anthocyanin biosynthesis in grape suspens...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-03-01
|
| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1351008/full |
| _version_ | 1797253693944365056 |
|---|---|
| author | Kainan Zhao Kainan Zhao Yibin Lan Yibin Lan Ying Shi Ying Shi Changqing Duan Changqing Duan Keji Yu Keji Yu Keji Yu |
| author_facet | Kainan Zhao Kainan Zhao Yibin Lan Yibin Lan Ying Shi Ying Shi Changqing Duan Changqing Duan Keji Yu Keji Yu Keji Yu |
| author_sort | Kainan Zhao |
| collection | DOAJ |
| description | Proanthocyanidins (PAs) and anthocyanins are flavonoids that contribute to the quality and health benefits of grapes and wine. Salinity affects their biosynthesis, but the underlying mechanism is still unclear. We studied the effects of NaCl stress on PA and anthocyanin biosynthesis in grape suspension cells derived from berry skins of Vitis vinifera L. Cabernet Sauvignon using metabolite profiling and transcriptome analysis. We treated the cells with low (75 mM NaCl) and high (150 mM NaCl) salinity for 4 and 7 days. High salinity inhibited cell growth and enhanced PA and anthocyanin accumulation more than low salinity. The salinity-induced PAs and anthocyanins lacked C5’-hydroxylation modification, suggesting the biological significance of delphinidin- and epigallocatechin-derivatives in coping with stress. The genes up-regulated by salinity stress indicated that the anthocyanin pathway was more sensitive to salt concentration than the PA pathway, and WGCNA analysis revealed the coordination between flavonoid biosynthesis and cell wall metabolism under salinity stress. We identified transcription factors potentially involved in regulating NaCl dose- and time-dependent PA and anthocyanin accumulation, showing the dynamic remodeling of flavonoid regulation network under different salinity levels and durations. Our study provides new insights into regulator candidates for tailoring flavonoid composition and molecular indicators of salt stress in grape cells. |
| first_indexed | 2024-04-24T21:38:07Z |
| format | Article |
| id | doaj.art-307c2d59a3e143e69800f02f8904e598 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-24T21:38:07Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-307c2d59a3e143e69800f02f8904e5982024-03-21T11:48:47ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-03-011510.3389/fpls.2024.13510081351008Metabolite and transcriptome analyses reveal the effects of salinity stress on the biosynthesis of proanthocyanidins and anthocyanins in grape suspension cellsKainan Zhao0Kainan Zhao1Yibin Lan2Yibin Lan3Ying Shi4Ying Shi5Changqing Duan6Changqing Duan7Keji Yu8Keji Yu9Keji Yu10Center for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaKey Laboratory of Viticulture and Enology, Ministry of Agriculture and Rural Affairs, Beijing, ChinaCenter for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaKey Laboratory of Viticulture and Enology, Ministry of Agriculture and Rural Affairs, Beijing, ChinaCenter for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaKey Laboratory of Viticulture and Enology, Ministry of Agriculture and Rural Affairs, Beijing, ChinaCenter for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaKey Laboratory of Viticulture and Enology, Ministry of Agriculture and Rural Affairs, Beijing, ChinaCenter for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, ChinaKey Laboratory of Viticulture and Enology, Ministry of Agriculture and Rural Affairs, Beijing, ChinaCollege of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, ChinaProanthocyanidins (PAs) and anthocyanins are flavonoids that contribute to the quality and health benefits of grapes and wine. Salinity affects their biosynthesis, but the underlying mechanism is still unclear. We studied the effects of NaCl stress on PA and anthocyanin biosynthesis in grape suspension cells derived from berry skins of Vitis vinifera L. Cabernet Sauvignon using metabolite profiling and transcriptome analysis. We treated the cells with low (75 mM NaCl) and high (150 mM NaCl) salinity for 4 and 7 days. High salinity inhibited cell growth and enhanced PA and anthocyanin accumulation more than low salinity. The salinity-induced PAs and anthocyanins lacked C5’-hydroxylation modification, suggesting the biological significance of delphinidin- and epigallocatechin-derivatives in coping with stress. The genes up-regulated by salinity stress indicated that the anthocyanin pathway was more sensitive to salt concentration than the PA pathway, and WGCNA analysis revealed the coordination between flavonoid biosynthesis and cell wall metabolism under salinity stress. We identified transcription factors potentially involved in regulating NaCl dose- and time-dependent PA and anthocyanin accumulation, showing the dynamic remodeling of flavonoid regulation network under different salinity levels and durations. Our study provides new insights into regulator candidates for tailoring flavonoid composition and molecular indicators of salt stress in grape cells.https://www.frontiersin.org/articles/10.3389/fpls.2024.1351008/fullgrapeproanthocyanidinsanthocyaninssalinitysuspension cellsflavonoid biosynthesis |
| spellingShingle | Kainan Zhao Kainan Zhao Yibin Lan Yibin Lan Ying Shi Ying Shi Changqing Duan Changqing Duan Keji Yu Keji Yu Keji Yu Metabolite and transcriptome analyses reveal the effects of salinity stress on the biosynthesis of proanthocyanidins and anthocyanins in grape suspension cells Frontiers in Plant Science grape proanthocyanidins anthocyanins salinity suspension cells flavonoid biosynthesis |
| title | Metabolite and transcriptome analyses reveal the effects of salinity stress on the biosynthesis of proanthocyanidins and anthocyanins in grape suspension cells |
| title_full | Metabolite and transcriptome analyses reveal the effects of salinity stress on the biosynthesis of proanthocyanidins and anthocyanins in grape suspension cells |
| title_fullStr | Metabolite and transcriptome analyses reveal the effects of salinity stress on the biosynthesis of proanthocyanidins and anthocyanins in grape suspension cells |
| title_full_unstemmed | Metabolite and transcriptome analyses reveal the effects of salinity stress on the biosynthesis of proanthocyanidins and anthocyanins in grape suspension cells |
| title_short | Metabolite and transcriptome analyses reveal the effects of salinity stress on the biosynthesis of proanthocyanidins and anthocyanins in grape suspension cells |
| title_sort | metabolite and transcriptome analyses reveal the effects of salinity stress on the biosynthesis of proanthocyanidins and anthocyanins in grape suspension cells |
| topic | grape proanthocyanidins anthocyanins salinity suspension cells flavonoid biosynthesis |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1351008/full |
| work_keys_str_mv | AT kainanzhao metaboliteandtranscriptomeanalysesrevealtheeffectsofsalinitystressonthebiosynthesisofproanthocyanidinsandanthocyaninsingrapesuspensioncells AT kainanzhao metaboliteandtranscriptomeanalysesrevealtheeffectsofsalinitystressonthebiosynthesisofproanthocyanidinsandanthocyaninsingrapesuspensioncells AT yibinlan metaboliteandtranscriptomeanalysesrevealtheeffectsofsalinitystressonthebiosynthesisofproanthocyanidinsandanthocyaninsingrapesuspensioncells AT yibinlan metaboliteandtranscriptomeanalysesrevealtheeffectsofsalinitystressonthebiosynthesisofproanthocyanidinsandanthocyaninsingrapesuspensioncells AT yingshi metaboliteandtranscriptomeanalysesrevealtheeffectsofsalinitystressonthebiosynthesisofproanthocyanidinsandanthocyaninsingrapesuspensioncells AT yingshi metaboliteandtranscriptomeanalysesrevealtheeffectsofsalinitystressonthebiosynthesisofproanthocyanidinsandanthocyaninsingrapesuspensioncells AT changqingduan metaboliteandtranscriptomeanalysesrevealtheeffectsofsalinitystressonthebiosynthesisofproanthocyanidinsandanthocyaninsingrapesuspensioncells AT changqingduan metaboliteandtranscriptomeanalysesrevealtheeffectsofsalinitystressonthebiosynthesisofproanthocyanidinsandanthocyaninsingrapesuspensioncells AT kejiyu metaboliteandtranscriptomeanalysesrevealtheeffectsofsalinitystressonthebiosynthesisofproanthocyanidinsandanthocyaninsingrapesuspensioncells AT kejiyu metaboliteandtranscriptomeanalysesrevealtheeffectsofsalinitystressonthebiosynthesisofproanthocyanidinsandanthocyaninsingrapesuspensioncells AT kejiyu metaboliteandtranscriptomeanalysesrevealtheeffectsofsalinitystressonthebiosynthesisofproanthocyanidinsandanthocyaninsingrapesuspensioncells |