Adipose mTORC2 is essential for sensory innervation in white adipose tissue and whole-body energy homeostasis
Objective: Adipose tissue, via sympathetic and possibly sensory neurons, communicates with the central nervous system (CNS) to mediate energy homeostasis. In contrast to the sympathetic nervous system, the morphology, role and regulation of the sensory nervous system in adipose tissue are poorly cha...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-11-01
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| Series: | Molecular Metabolism |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212877822001491 |
| _version_ | 1797997390123237376 |
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| author | Irina C. Frei Diana Weissenberger Danilo Ritz Wolf Heusermann Marco Colombi Mitsugu Shimobayashi Michael N. Hall |
| author_facet | Irina C. Frei Diana Weissenberger Danilo Ritz Wolf Heusermann Marco Colombi Mitsugu Shimobayashi Michael N. Hall |
| author_sort | Irina C. Frei |
| collection | DOAJ |
| description | Objective: Adipose tissue, via sympathetic and possibly sensory neurons, communicates with the central nervous system (CNS) to mediate energy homeostasis. In contrast to the sympathetic nervous system, the morphology, role and regulation of the sensory nervous system in adipose tissue are poorly characterized. Methods and results: Taking advantage of recent progress in whole-mount three-dimensional imaging, we identified a network of calcitonin gene-related protein (CGRP)-positive sensory neurons in murine white adipose tissue (WAT). We found that adipose mammalian target of rapamycin complex 2 (mTORC2), a major component of the insulin signaling pathway, is required for arborization of sensory neurons, but not of sympathetic neurons. Time course experiments revealed that adipose mTORC2 is required for maintenance of sensory neurons. Furthermore, loss of sensory innervation in WAT coincided with systemic insulin resistance. Finally, we established that neuronal protein growth-associated protein 43 (GAP43) is a marker for sensory neurons in adipose tissue. Conclusion: Our findings indicate that adipose mTORC2 is necessary for sensory innervation in WAT. In addition, our results suggest that WAT may affect whole-body energy homeostasis via sensory neurons. |
| first_indexed | 2024-04-11T10:32:12Z |
| format | Article |
| id | doaj.art-30829a38ac744f36b7edab0306e721a5 |
| institution | Directory Open Access Journal |
| issn | 2212-8778 |
| language | English |
| last_indexed | 2024-04-11T10:32:12Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Metabolism |
| spelling | doaj.art-30829a38ac744f36b7edab0306e721a52022-12-22T04:29:24ZengElsevierMolecular Metabolism2212-87782022-11-0165101580Adipose mTORC2 is essential for sensory innervation in white adipose tissue and whole-body energy homeostasisIrina C. Frei0Diana Weissenberger1Danilo Ritz2Wolf Heusermann3Marco Colombi4Mitsugu Shimobayashi5Michael N. Hall6Biozentrum, University of Basel, Basel 4056, SwitzerlandBiozentrum, University of Basel, Basel 4056, SwitzerlandBiozentrum, University of Basel, Basel 4056, SwitzerlandBiozentrum, University of Basel, Basel 4056, SwitzerlandBiozentrum, University of Basel, Basel 4056, SwitzerlandCorresponding author.; Biozentrum, University of Basel, Basel 4056, SwitzerlandCorresponding author.; Biozentrum, University of Basel, Basel 4056, SwitzerlandObjective: Adipose tissue, via sympathetic and possibly sensory neurons, communicates with the central nervous system (CNS) to mediate energy homeostasis. In contrast to the sympathetic nervous system, the morphology, role and regulation of the sensory nervous system in adipose tissue are poorly characterized. Methods and results: Taking advantage of recent progress in whole-mount three-dimensional imaging, we identified a network of calcitonin gene-related protein (CGRP)-positive sensory neurons in murine white adipose tissue (WAT). We found that adipose mammalian target of rapamycin complex 2 (mTORC2), a major component of the insulin signaling pathway, is required for arborization of sensory neurons, but not of sympathetic neurons. Time course experiments revealed that adipose mTORC2 is required for maintenance of sensory neurons. Furthermore, loss of sensory innervation in WAT coincided with systemic insulin resistance. Finally, we established that neuronal protein growth-associated protein 43 (GAP43) is a marker for sensory neurons in adipose tissue. Conclusion: Our findings indicate that adipose mTORC2 is necessary for sensory innervation in WAT. In addition, our results suggest that WAT may affect whole-body energy homeostasis via sensory neurons.http://www.sciencedirect.com/science/article/pii/S2212877822001491Adipose tissueWhole-body energy homeostasismTORC2Sensory nervous systemDiabetesCGRP |
| spellingShingle | Irina C. Frei Diana Weissenberger Danilo Ritz Wolf Heusermann Marco Colombi Mitsugu Shimobayashi Michael N. Hall Adipose mTORC2 is essential for sensory innervation in white adipose tissue and whole-body energy homeostasis Molecular Metabolism Adipose tissue Whole-body energy homeostasis mTORC2 Sensory nervous system Diabetes CGRP |
| title | Adipose mTORC2 is essential for sensory innervation in white adipose tissue and whole-body energy homeostasis |
| title_full | Adipose mTORC2 is essential for sensory innervation in white adipose tissue and whole-body energy homeostasis |
| title_fullStr | Adipose mTORC2 is essential for sensory innervation in white adipose tissue and whole-body energy homeostasis |
| title_full_unstemmed | Adipose mTORC2 is essential for sensory innervation in white adipose tissue and whole-body energy homeostasis |
| title_short | Adipose mTORC2 is essential for sensory innervation in white adipose tissue and whole-body energy homeostasis |
| title_sort | adipose mtorc2 is essential for sensory innervation in white adipose tissue and whole body energy homeostasis |
| topic | Adipose tissue Whole-body energy homeostasis mTORC2 Sensory nervous system Diabetes CGRP |
| url | http://www.sciencedirect.com/science/article/pii/S2212877822001491 |
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