Barley Leaf Ameliorates <i>Citrobacter-rodentium</i>-Induced Colitis through Arginine Enrichment
Inflammatory bowel disease (IBD) has become a global public health challenge. Our previous study showed that barley leaf (BL) significantly reduces <i>Citrobacter-rodentium</i> (CR)-induced colitis, but its mechanism remains elusive. Thus, in this study, we used non-targeted metabolomics...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-04-01
|
| Series: | Nutrients |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6643/15/8/1890 |
| _version_ | 1797604008493318144 |
|---|---|
| author | Yu Feng Daotong Li Chen Ma Xiaosong Hu Fang Chen |
| author_facet | Yu Feng Daotong Li Chen Ma Xiaosong Hu Fang Chen |
| author_sort | Yu Feng |
| collection | DOAJ |
| description | Inflammatory bowel disease (IBD) has become a global public health challenge. Our previous study showed that barley leaf (BL) significantly reduces <i>Citrobacter-rodentium</i> (CR)-induced colitis, but its mechanism remains elusive. Thus, in this study, we used non-targeted metabolomics techniques to search for potentially effective metabolites. Our results demonstrated that dietary supplementation with BL significantly enriched arginine and that arginine intervention significantly ameliorated CR-induced colitis symptoms such as reduced body weight, shortened colon, wrinkled cecum, and swollen colon wall in mice; in addition, arginine intervention dramatically ameliorated CR-induced histopathological damage to the colon. The gut microbial diversity analysis showed that arginine intervention significantly decreased the relative abundance of CR and significantly increased the relative abundance of <i>Akkermansia</i>, <i>Blautia</i>, <i>Enterorhabdus</i>, and <i>Lachnospiraceae</i>, which modified the CR-induced intestinal flora disorder. Notably, arginine showed a dose-dependent effect on the improvement of colitis caused by CR. |
| first_indexed | 2024-03-11T04:40:08Z |
| format | Article |
| id | doaj.art-3088212f92694a24970dcac88a702935 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-11T04:40:08Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-3088212f92694a24970dcac88a7029352023-11-17T20:45:56ZengMDPI AGNutrients2072-66432023-04-01158189010.3390/nu15081890Barley Leaf Ameliorates <i>Citrobacter-rodentium</i>-Induced Colitis through Arginine EnrichmentYu Feng0Daotong Li1Chen Ma2Xiaosong Hu3Fang Chen4College of Food Science and Nutritional Engineering, National Engineering Research Center for Fruit and Vegetable Processing, Key Laboratory of Fruit and Vegetables Processing Ministry of Agriculture, Engineering Research Centre for Engineering Vegetables Processing, Ministry of Education, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, National Engineering Research Center for Fruit and Vegetable Processing, Key Laboratory of Fruit and Vegetables Processing Ministry of Agriculture, Engineering Research Centre for Engineering Vegetables Processing, Ministry of Education, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, National Engineering Research Center for Fruit and Vegetable Processing, Key Laboratory of Fruit and Vegetables Processing Ministry of Agriculture, Engineering Research Centre for Engineering Vegetables Processing, Ministry of Education, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, National Engineering Research Center for Fruit and Vegetable Processing, Key Laboratory of Fruit and Vegetables Processing Ministry of Agriculture, Engineering Research Centre for Engineering Vegetables Processing, Ministry of Education, China Agricultural University, Beijing 100083, ChinaCollege of Food Science and Nutritional Engineering, National Engineering Research Center for Fruit and Vegetable Processing, Key Laboratory of Fruit and Vegetables Processing Ministry of Agriculture, Engineering Research Centre for Engineering Vegetables Processing, Ministry of Education, China Agricultural University, Beijing 100083, ChinaInflammatory bowel disease (IBD) has become a global public health challenge. Our previous study showed that barley leaf (BL) significantly reduces <i>Citrobacter-rodentium</i> (CR)-induced colitis, but its mechanism remains elusive. Thus, in this study, we used non-targeted metabolomics techniques to search for potentially effective metabolites. Our results demonstrated that dietary supplementation with BL significantly enriched arginine and that arginine intervention significantly ameliorated CR-induced colitis symptoms such as reduced body weight, shortened colon, wrinkled cecum, and swollen colon wall in mice; in addition, arginine intervention dramatically ameliorated CR-induced histopathological damage to the colon. The gut microbial diversity analysis showed that arginine intervention significantly decreased the relative abundance of CR and significantly increased the relative abundance of <i>Akkermansia</i>, <i>Blautia</i>, <i>Enterorhabdus</i>, and <i>Lachnospiraceae</i>, which modified the CR-induced intestinal flora disorder. Notably, arginine showed a dose-dependent effect on the improvement of colitis caused by CR.https://www.mdpi.com/2072-6643/15/8/1890IBDBL<i>Citrobacter rodentium</i>argininegut microbiota<i>Akkermansia</i> |
| spellingShingle | Yu Feng Daotong Li Chen Ma Xiaosong Hu Fang Chen Barley Leaf Ameliorates <i>Citrobacter-rodentium</i>-Induced Colitis through Arginine Enrichment Nutrients IBD BL <i>Citrobacter rodentium</i> arginine gut microbiota <i>Akkermansia</i> |
| title | Barley Leaf Ameliorates <i>Citrobacter-rodentium</i>-Induced Colitis through Arginine Enrichment |
| title_full | Barley Leaf Ameliorates <i>Citrobacter-rodentium</i>-Induced Colitis through Arginine Enrichment |
| title_fullStr | Barley Leaf Ameliorates <i>Citrobacter-rodentium</i>-Induced Colitis through Arginine Enrichment |
| title_full_unstemmed | Barley Leaf Ameliorates <i>Citrobacter-rodentium</i>-Induced Colitis through Arginine Enrichment |
| title_short | Barley Leaf Ameliorates <i>Citrobacter-rodentium</i>-Induced Colitis through Arginine Enrichment |
| title_sort | barley leaf ameliorates i citrobacter rodentium i induced colitis through arginine enrichment |
| topic | IBD BL <i>Citrobacter rodentium</i> arginine gut microbiota <i>Akkermansia</i> |
| url | https://www.mdpi.com/2072-6643/15/8/1890 |
| work_keys_str_mv | AT yufeng barleyleafamelioratesicitrobacterrodentiumiinducedcolitisthrougharginineenrichment AT daotongli barleyleafamelioratesicitrobacterrodentiumiinducedcolitisthrougharginineenrichment AT chenma barleyleafamelioratesicitrobacterrodentiumiinducedcolitisthrougharginineenrichment AT xiaosonghu barleyleafamelioratesicitrobacterrodentiumiinducedcolitisthrougharginineenrichment AT fangchen barleyleafamelioratesicitrobacterrodentiumiinducedcolitisthrougharginineenrichment |