Distinct Expression Patterns of Osteopontin and Dentin Matrix Protein 1 Genes in Pituitary Gonadotrophs
Cell-matrix interactions play important roles in pituitary development, physiology, and pathogenesis. In other tissues, a family of non-collagenous proteins, termed SIBLINGs, are known to contribute to cell-matrix interactions. Anterior pituitary gland expresses two SIBLING genes, Dmp1 (dentin matri...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-04-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fendo.2019.00248/full |
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| author | Ivana Bjelobaba Ivana Bjelobaba Marija M. Janjic Marija M. Janjic Rafael Maso Prévide Daniel Abebe Marek Kucka Stanko S. Stojilkovic |
| author_facet | Ivana Bjelobaba Ivana Bjelobaba Marija M. Janjic Marija M. Janjic Rafael Maso Prévide Daniel Abebe Marek Kucka Stanko S. Stojilkovic |
| author_sort | Ivana Bjelobaba |
| collection | DOAJ |
| description | Cell-matrix interactions play important roles in pituitary development, physiology, and pathogenesis. In other tissues, a family of non-collagenous proteins, termed SIBLINGs, are known to contribute to cell-matrix interactions. Anterior pituitary gland expresses two SIBLING genes, Dmp1 (dentin matrix protein-1) and Spp1 (secreted phosphoprotein-1) encoding DMP1 and osteopontin proteins, respectively, but their expression pattern and roles in pituitary functions have not been clarified. Here we provide novel evidence supporting the conclusion that Spp1/osteopontin, like Dmp1/DMP1, are expressed in gonadotrophs in a sex- and age-specific manner. Other anterior pituitary cell types do not express these genes. In contrast to Dmp1, Spp1 expression is higher in males; in females, the expression reaches the peak during the diestrus phase of estrous cycle. In further contrast to Dmp1 and marker genes for gonadotrophs, the expression of Spp1 is not regulated by gonadotropin-releasing hormone in vivo and in vitro. However, Spp1 expression increases progressively after pituitary cell dispersion in both female and male cultures. We may speculate that gonadotrophs signal to other pituitary cell types about changes in the structure of pituitary cell-matrix network by osteopontin, a function consistent with the role of this secretory protein in postnatal tissue remodeling, extracellular matrix reorganization after injury, and tumorigenesis. |
| first_indexed | 2024-04-14T01:40:14Z |
| format | Article |
| id | doaj.art-308a35a1e52d448f823bed8782b12af9 |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-14T01:40:14Z |
| publishDate | 2019-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-308a35a1e52d448f823bed8782b12af92022-12-22T02:19:48ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922019-04-011010.3389/fendo.2019.00248452715Distinct Expression Patterns of Osteopontin and Dentin Matrix Protein 1 Genes in Pituitary GonadotrophsIvana Bjelobaba0Ivana Bjelobaba1Marija M. Janjic2Marija M. Janjic3Rafael Maso Prévide4Daniel Abebe5Marek Kucka6Stanko S. Stojilkovic7Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health (NIH), Bethesda, MD, United StatesInstitute for Biological Research Sinisa Stankovic, University of Belgrade, Belgrade, SerbiaEunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health (NIH), Bethesda, MD, United StatesInstitute for Biological Research Sinisa Stankovic, University of Belgrade, Belgrade, SerbiaEunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health (NIH), Bethesda, MD, United StatesEunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health (NIH), Bethesda, MD, United StatesEunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health (NIH), Bethesda, MD, United StatesEunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health (NIH), Bethesda, MD, United StatesCell-matrix interactions play important roles in pituitary development, physiology, and pathogenesis. In other tissues, a family of non-collagenous proteins, termed SIBLINGs, are known to contribute to cell-matrix interactions. Anterior pituitary gland expresses two SIBLING genes, Dmp1 (dentin matrix protein-1) and Spp1 (secreted phosphoprotein-1) encoding DMP1 and osteopontin proteins, respectively, but their expression pattern and roles in pituitary functions have not been clarified. Here we provide novel evidence supporting the conclusion that Spp1/osteopontin, like Dmp1/DMP1, are expressed in gonadotrophs in a sex- and age-specific manner. Other anterior pituitary cell types do not express these genes. In contrast to Dmp1, Spp1 expression is higher in males; in females, the expression reaches the peak during the diestrus phase of estrous cycle. In further contrast to Dmp1 and marker genes for gonadotrophs, the expression of Spp1 is not regulated by gonadotropin-releasing hormone in vivo and in vitro. However, Spp1 expression increases progressively after pituitary cell dispersion in both female and male cultures. We may speculate that gonadotrophs signal to other pituitary cell types about changes in the structure of pituitary cell-matrix network by osteopontin, a function consistent with the role of this secretory protein in postnatal tissue remodeling, extracellular matrix reorganization after injury, and tumorigenesis.https://www.frontiersin.org/article/10.3389/fendo.2019.00248/fullpituitarygonadotrophsSIBLINGsSpp1osteopontinDmp1 |
| spellingShingle | Ivana Bjelobaba Ivana Bjelobaba Marija M. Janjic Marija M. Janjic Rafael Maso Prévide Daniel Abebe Marek Kucka Stanko S. Stojilkovic Distinct Expression Patterns of Osteopontin and Dentin Matrix Protein 1 Genes in Pituitary Gonadotrophs Frontiers in Endocrinology pituitary gonadotrophs SIBLINGs Spp1 osteopontin Dmp1 |
| title | Distinct Expression Patterns of Osteopontin and Dentin Matrix Protein 1 Genes in Pituitary Gonadotrophs |
| title_full | Distinct Expression Patterns of Osteopontin and Dentin Matrix Protein 1 Genes in Pituitary Gonadotrophs |
| title_fullStr | Distinct Expression Patterns of Osteopontin and Dentin Matrix Protein 1 Genes in Pituitary Gonadotrophs |
| title_full_unstemmed | Distinct Expression Patterns of Osteopontin and Dentin Matrix Protein 1 Genes in Pituitary Gonadotrophs |
| title_short | Distinct Expression Patterns of Osteopontin and Dentin Matrix Protein 1 Genes in Pituitary Gonadotrophs |
| title_sort | distinct expression patterns of osteopontin and dentin matrix protein 1 genes in pituitary gonadotrophs |
| topic | pituitary gonadotrophs SIBLINGs Spp1 osteopontin Dmp1 |
| url | https://www.frontiersin.org/article/10.3389/fendo.2019.00248/full |
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