Targeting Fluorescence Imaging of RGD-Modified Indocyanine Green Micelles on Gastric Cancer
Early diagnosis and complete resection of the tumor is an important way to improve the quality of life of patients with gastric cancer. In recent years, near-infrared (NIR) materials show great potential in fluorescence-based imaging of the tumors. To realize a satisfying intraoperative fluorescence...
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| Format: | Article |
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Frontiers Media S.A.
2020-09-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2020.575365/full |
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| author | Jun Shao Xiaoming Zheng Longbao Feng Tianyun Lan Dongbing Ding Zikai Cai Xudong Zhu Rongpu Liang Bo Wei |
| author_facet | Jun Shao Xiaoming Zheng Longbao Feng Tianyun Lan Dongbing Ding Zikai Cai Xudong Zhu Rongpu Liang Bo Wei |
| author_sort | Jun Shao |
| collection | DOAJ |
| description | Early diagnosis and complete resection of the tumor is an important way to improve the quality of life of patients with gastric cancer. In recent years, near-infrared (NIR) materials show great potential in fluorescence-based imaging of the tumors. To realize a satisfying intraoperative fluorescence tumor imaging, there are two pre-requirements. One is to obtain a stable agent with a relatively longer circulation time. The second is to make it good biocompatible and specific targeting to the tumor. Here, we developed an RGD-modified Distearyl acylphosphatidyl ethanolamine-polyethylene glycol micelle (DSPE-PEG-RGD) to encapsulate indocyanine green (ICG) for targeting fluorescence imaging of gastric cancer, aimed at realizing tumor-targeted accumulation and NIR imaging. 1H NMR spectroscopy confirmed its molecular structure. The characteristics and stability results indicated that the DSPE-PEG-RGD@ICG had a relatively uniform size of <200 nm and longer-term fluorescence stability. RGD peptides had a high affinity to integrin αvβ3 and the specific targeting effect on SGC7901 was assessed by confocal microscopy in vitro. Additionally, the results of cytotoxicity and blood compatibility in vitro were consistent with the acute toxicity test in vivo, which revealed good biocompatibility. The biodistribution and tumor targeting image of DSPE-PEG-RGD@ICG were observed by an imaging system in tumor-bearing mice. DSPE-PEG-RGD@ICG demonstrated an improved accumulation in tumors and longer circulation time when compared with free ICG or DSPE-PEG@ICG. In all, DSPE-PEG-RGD@ICG demonstrated ideal properties for tumor target imaging, thus, providing a promising way for the detection and accurate resection of gastric cancer. |
| first_indexed | 2024-12-11T10:00:20Z |
| format | Article |
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| language | English |
| last_indexed | 2024-12-11T10:00:20Z |
| publishDate | 2020-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-308ca7a232bc4140be5712f8cd6543682022-12-22T01:12:08ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-09-01810.3389/fbioe.2020.575365575365Targeting Fluorescence Imaging of RGD-Modified Indocyanine Green Micelles on Gastric CancerJun Shao0Xiaoming Zheng1Longbao Feng2Tianyun Lan3Dongbing Ding4Zikai Cai5Xudong Zhu6Rongpu Liang7Bo Wei8Department of Gastrointestinal Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaDepartment of Gastrointestinal Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaDepartment of Biomedical Engineering, Ji’nan University, Guangzhou, ChinaCentral Laboratory, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaDepartment of Gastrointestinal Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaDepartment of Gastrointestinal Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaDepartment of Gastrointestinal Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaDepartment of Gastrointestinal Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaDepartment of Gastrointestinal Surgery, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaEarly diagnosis and complete resection of the tumor is an important way to improve the quality of life of patients with gastric cancer. In recent years, near-infrared (NIR) materials show great potential in fluorescence-based imaging of the tumors. To realize a satisfying intraoperative fluorescence tumor imaging, there are two pre-requirements. One is to obtain a stable agent with a relatively longer circulation time. The second is to make it good biocompatible and specific targeting to the tumor. Here, we developed an RGD-modified Distearyl acylphosphatidyl ethanolamine-polyethylene glycol micelle (DSPE-PEG-RGD) to encapsulate indocyanine green (ICG) for targeting fluorescence imaging of gastric cancer, aimed at realizing tumor-targeted accumulation and NIR imaging. 1H NMR spectroscopy confirmed its molecular structure. The characteristics and stability results indicated that the DSPE-PEG-RGD@ICG had a relatively uniform size of <200 nm and longer-term fluorescence stability. RGD peptides had a high affinity to integrin αvβ3 and the specific targeting effect on SGC7901 was assessed by confocal microscopy in vitro. Additionally, the results of cytotoxicity and blood compatibility in vitro were consistent with the acute toxicity test in vivo, which revealed good biocompatibility. The biodistribution and tumor targeting image of DSPE-PEG-RGD@ICG were observed by an imaging system in tumor-bearing mice. DSPE-PEG-RGD@ICG demonstrated an improved accumulation in tumors and longer circulation time when compared with free ICG or DSPE-PEG@ICG. In all, DSPE-PEG-RGD@ICG demonstrated ideal properties for tumor target imaging, thus, providing a promising way for the detection and accurate resection of gastric cancer.https://www.frontiersin.org/article/10.3389/fbioe.2020.575365/fullgastric cancermicellesRGDindocyanine greentargeting |
| spellingShingle | Jun Shao Xiaoming Zheng Longbao Feng Tianyun Lan Dongbing Ding Zikai Cai Xudong Zhu Rongpu Liang Bo Wei Targeting Fluorescence Imaging of RGD-Modified Indocyanine Green Micelles on Gastric Cancer Frontiers in Bioengineering and Biotechnology gastric cancer micelles RGD indocyanine green targeting |
| title | Targeting Fluorescence Imaging of RGD-Modified Indocyanine Green Micelles on Gastric Cancer |
| title_full | Targeting Fluorescence Imaging of RGD-Modified Indocyanine Green Micelles on Gastric Cancer |
| title_fullStr | Targeting Fluorescence Imaging of RGD-Modified Indocyanine Green Micelles on Gastric Cancer |
| title_full_unstemmed | Targeting Fluorescence Imaging of RGD-Modified Indocyanine Green Micelles on Gastric Cancer |
| title_short | Targeting Fluorescence Imaging of RGD-Modified Indocyanine Green Micelles on Gastric Cancer |
| title_sort | targeting fluorescence imaging of rgd modified indocyanine green micelles on gastric cancer |
| topic | gastric cancer micelles RGD indocyanine green targeting |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2020.575365/full |
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