The anti-inflammatory SSEDIKE peptide from Amaranth seeds modulates IgE-mediated food allergy
The in vivo immunomodulatory effect of a bioactive peptide from Amaranthus hypochondriacus grains in an IgE-mediated food allergy mouse model was examined. The synthetic SSEDIKE peptide was orally administered to prevent food allergy. Upon oral challenge with milk allergens we observed an alleviatio...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016-08-01
|
| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464616301748 |
| _version_ | 1818596531121422336 |
|---|---|
| author | Julian Moronta Paola L. Smaldini Carlos A. Fossati María C. Añon Guillermo H. Docena |
| author_facet | Julian Moronta Paola L. Smaldini Carlos A. Fossati María C. Añon Guillermo H. Docena |
| author_sort | Julian Moronta |
| collection | DOAJ |
| description | The in vivo immunomodulatory effect of a bioactive peptide from Amaranthus hypochondriacus grains in an IgE-mediated food allergy mouse model was examined. The synthetic SSEDIKE peptide was orally administered to prevent food allergy. Upon oral challenge with milk allergens we observed an alleviation of hypersensitivity symptoms with negativization of skin test, a significant inhibition of specific IgE and IgG1 secretion, a substantial reduction in the secretion of IL-5 and IL-13 by antigen-stimulated spleen cells and mucosal reduction of transcripts coding for ccl20, gata-3 and nf-kb. In addition tgf-β and foxp3 mRNA levels were up-regulated in jejunum. The charged SSEDIKE peptide from Amaranth inhibited the allergy reaction in a mouse model of food allergy, with suppression of IgE secretion and control of the intestinal inflammation preventing the activation of NF-κB, which might be attributed to the induction of local tolerance. |
| first_indexed | 2024-12-16T11:33:23Z |
| format | Article |
| id | doaj.art-308fbffe2cbb45809e9aca21b0a7a398 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-16T11:33:23Z |
| publishDate | 2016-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-308fbffe2cbb45809e9aca21b0a7a3982022-12-21T22:33:10ZengElsevierJournal of Functional Foods1756-46462016-08-0125579587The anti-inflammatory SSEDIKE peptide from Amaranth seeds modulates IgE-mediated food allergyJulian Moronta0Paola L. Smaldini1Carlos A. Fossati2María C. Añon3Guillermo H. Docena4Centro de Investigación y Desarrollo en Tecnología de Alimentos (CIDCA), CONICET-CCT La Plata y Universidad Nacional de La Plata, La Plata, ArgentinaInstituto de Estudios Inmunológicos y Fisiopatológicos-IIFP, Facultad de Ciencias Exactas, Universidad Nacional de La Plata y Consejo Nacional de Investigaciones Científicas y Técnicas, La Plata, ArgentinaInstituto de Estudios Inmunológicos y Fisiopatológicos-IIFP, Facultad de Ciencias Exactas, Universidad Nacional de La Plata y Consejo Nacional de Investigaciones Científicas y Técnicas, La Plata, ArgentinaCentro de Investigación y Desarrollo en Tecnología de Alimentos (CIDCA), CONICET-CCT La Plata y Universidad Nacional de La Plata, La Plata, ArgentinaInstituto de Estudios Inmunológicos y Fisiopatológicos-IIFP, Facultad de Ciencias Exactas, Universidad Nacional de La Plata y Consejo Nacional de Investigaciones Científicas y Técnicas, La Plata, Argentina; Corresponding author. Instituto de Estudios Inmunológicos y Fisiopatológicos – IIFP, Facultad de Ciencias Exactas, Departamento de Ciencias Biológicas, Universidad de La Plata, Calles 47 y 115, La Plata 1900, Argentina. Fax: +54 221 422 6947.The in vivo immunomodulatory effect of a bioactive peptide from Amaranthus hypochondriacus grains in an IgE-mediated food allergy mouse model was examined. The synthetic SSEDIKE peptide was orally administered to prevent food allergy. Upon oral challenge with milk allergens we observed an alleviation of hypersensitivity symptoms with negativization of skin test, a significant inhibition of specific IgE and IgG1 secretion, a substantial reduction in the secretion of IL-5 and IL-13 by antigen-stimulated spleen cells and mucosal reduction of transcripts coding for ccl20, gata-3 and nf-kb. In addition tgf-β and foxp3 mRNA levels were up-regulated in jejunum. The charged SSEDIKE peptide from Amaranth inhibited the allergy reaction in a mouse model of food allergy, with suppression of IgE secretion and control of the intestinal inflammation preventing the activation of NF-κB, which might be attributed to the induction of local tolerance.http://www.sciencedirect.com/science/article/pii/S1756464616301748AmaranthAnti-inflammatory effectFood allergyIgEPeptides |
| spellingShingle | Julian Moronta Paola L. Smaldini Carlos A. Fossati María C. Añon Guillermo H. Docena The anti-inflammatory SSEDIKE peptide from Amaranth seeds modulates IgE-mediated food allergy Journal of Functional Foods Amaranth Anti-inflammatory effect Food allergy IgE Peptides |
| title | The anti-inflammatory SSEDIKE peptide from Amaranth seeds modulates IgE-mediated food allergy |
| title_full | The anti-inflammatory SSEDIKE peptide from Amaranth seeds modulates IgE-mediated food allergy |
| title_fullStr | The anti-inflammatory SSEDIKE peptide from Amaranth seeds modulates IgE-mediated food allergy |
| title_full_unstemmed | The anti-inflammatory SSEDIKE peptide from Amaranth seeds modulates IgE-mediated food allergy |
| title_short | The anti-inflammatory SSEDIKE peptide from Amaranth seeds modulates IgE-mediated food allergy |
| title_sort | anti inflammatory ssedike peptide from amaranth seeds modulates ige mediated food allergy |
| topic | Amaranth Anti-inflammatory effect Food allergy IgE Peptides |
| url | http://www.sciencedirect.com/science/article/pii/S1756464616301748 |
| work_keys_str_mv | AT julianmoronta theantiinflammatoryssedikepeptidefromamaranthseedsmodulatesigemediatedfoodallergy AT paolalsmaldini theantiinflammatoryssedikepeptidefromamaranthseedsmodulatesigemediatedfoodallergy AT carlosafossati theantiinflammatoryssedikepeptidefromamaranthseedsmodulatesigemediatedfoodallergy AT mariacanon theantiinflammatoryssedikepeptidefromamaranthseedsmodulatesigemediatedfoodallergy AT guillermohdocena theantiinflammatoryssedikepeptidefromamaranthseedsmodulatesigemediatedfoodallergy AT julianmoronta antiinflammatoryssedikepeptidefromamaranthseedsmodulatesigemediatedfoodallergy AT paolalsmaldini antiinflammatoryssedikepeptidefromamaranthseedsmodulatesigemediatedfoodallergy AT carlosafossati antiinflammatoryssedikepeptidefromamaranthseedsmodulatesigemediatedfoodallergy AT mariacanon antiinflammatoryssedikepeptidefromamaranthseedsmodulatesigemediatedfoodallergy AT guillermohdocena antiinflammatoryssedikepeptidefromamaranthseedsmodulatesigemediatedfoodallergy |