Effects of three types of fresh Rehmannia glutinosa improve lipopolysaccharide-induced acute kidney injury in sepsis through the estrogen receptor pathway
Objective(s): To explore the effects and mechanism of three types of fresh Rehmannia glutinosa, namely Beijing No. 3 (BJ3H), Huaizhong No. 1 (HZ1H), and Taisheng (TS) on lipopolysaccharide (LPS)-induced acute kidney injury in the sepsis (S-AKI) mice model through the estrogen receptor pathway.Materi...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2023-05-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | https://ijbms.mums.ac.ir/article_21875_f0ea3eaed41fdefb514b3d00dd5d4f68.pdf |
| _version_ | 1797848892821209088 |
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| author | Meng Liu Mengnan Zeng Pengli Guo Yuhan Zhang Xiaofeng Yang Jufang Jia Qinqin Zhang Beibei Zhang Bing Cao Ru Wang Xiaoke Zheng Weisheng Feng |
| author_facet | Meng Liu Mengnan Zeng Pengli Guo Yuhan Zhang Xiaofeng Yang Jufang Jia Qinqin Zhang Beibei Zhang Bing Cao Ru Wang Xiaoke Zheng Weisheng Feng |
| author_sort | Meng Liu |
| collection | DOAJ |
| description | Objective(s): To explore the effects and mechanism of three types of fresh Rehmannia glutinosa, namely Beijing No. 3 (BJ3H), Huaizhong No. 1 (HZ1H), and Taisheng (TS) on lipopolysaccharide (LPS)-induced acute kidney injury in the sepsis (S-AKI) mice model through the estrogen receptor pathway.Materials and Methods: BALB/c mice were randomly divided into control (CON), model (LPS), astragalus injection (ASI), BJ3H, HZ1H, TS water extract groups, the estrogen receptor antagonist ICI182,780 groups were added to each group. The antagonist groups received an intraperitoneal injection of ICI 0.5 hr before administration and an intraperitoneal injection of LPS 3 days after administration. The kidney pathology, function, inflammatory factors, immune cells, levels of reactive oxygen species (ROS), apoptosis, and the protein expression levels of TLR4/NF-κB/NLRP3 signaling pathway in the mice kidneys were detected.Results: ASI, BJ3H, HZ1H, and TS improved LPS-induced renal pathology in S-AKI mice, reduced the kidney and serum levels of inflammatory factors, positive rates of macrophages and neutrophils, levels of ROS and apoptosis, and the relative expression levels of TLR4, MyD88, NF-κB p-p65/NF-κB p65, and NLRP3 proteins in the kidney. In addition, they increased the positive rate of dendritic cells (DCs) in the mice kidneys. The overall effect of HZ1H was superior to that of ASI, BJ3H, and TS. However, after adding ICI, the regulatory effects of drugs were inhibited.Conclusion: The three types of fresh R. glutinosa may completely or partially affect the TLR4/NF-κB/NLRP3 signaling pathway through the estrogen receptor pathway to exert a protective effect on S-AKI. |
| first_indexed | 2024-04-09T18:36:03Z |
| format | Article |
| id | doaj.art-30914cadc44b4799b875192fd2272d94 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-09T18:36:03Z |
| publishDate | 2023-05-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-30914cadc44b4799b875192fd2272d942023-04-11T10:06:54ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742023-05-0126557958610.22038/ijbms.2023.67322.1475721875Effects of three types of fresh Rehmannia glutinosa improve lipopolysaccharide-induced acute kidney injury in sepsis through the estrogen receptor pathwayMeng Liu0Mengnan Zeng1Pengli Guo2Yuhan Zhang3Xiaofeng Yang4Jufang Jia5Qinqin Zhang6Beibei Zhang7Bing Cao8Ru Wang9Xiaoke Zheng10Weisheng Feng11Department of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, ChinaDepartment of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, ChinaDepartment of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, ChinaDepartment of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, ChinaDepartment of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, ChinaDepartment of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, ChinaDepartment of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, ChinaDepartment of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, ChinaDepartment of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, ChinaDepartment of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, ChinaDepartment of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, ChinaDepartment of Medicine, Henan University of Chinese Medicine, Zhengzhou 450046, ChinaObjective(s): To explore the effects and mechanism of three types of fresh Rehmannia glutinosa, namely Beijing No. 3 (BJ3H), Huaizhong No. 1 (HZ1H), and Taisheng (TS) on lipopolysaccharide (LPS)-induced acute kidney injury in the sepsis (S-AKI) mice model through the estrogen receptor pathway.Materials and Methods: BALB/c mice were randomly divided into control (CON), model (LPS), astragalus injection (ASI), BJ3H, HZ1H, TS water extract groups, the estrogen receptor antagonist ICI182,780 groups were added to each group. The antagonist groups received an intraperitoneal injection of ICI 0.5 hr before administration and an intraperitoneal injection of LPS 3 days after administration. The kidney pathology, function, inflammatory factors, immune cells, levels of reactive oxygen species (ROS), apoptosis, and the protein expression levels of TLR4/NF-κB/NLRP3 signaling pathway in the mice kidneys were detected.Results: ASI, BJ3H, HZ1H, and TS improved LPS-induced renal pathology in S-AKI mice, reduced the kidney and serum levels of inflammatory factors, positive rates of macrophages and neutrophils, levels of ROS and apoptosis, and the relative expression levels of TLR4, MyD88, NF-κB p-p65/NF-κB p65, and NLRP3 proteins in the kidney. In addition, they increased the positive rate of dendritic cells (DCs) in the mice kidneys. The overall effect of HZ1H was superior to that of ASI, BJ3H, and TS. However, after adding ICI, the regulatory effects of drugs were inhibited.Conclusion: The three types of fresh R. glutinosa may completely or partially affect the TLR4/NF-κB/NLRP3 signaling pathway through the estrogen receptor pathway to exert a protective effect on S-AKI.https://ijbms.mums.ac.ir/article_21875_f0ea3eaed41fdefb514b3d00dd5d4f68.pdfacute kidney injuryestrogenfresh rehmannia glutinosalipopolysaccharidenf-kappa bnlrp3sepsistlr4 |
| spellingShingle | Meng Liu Mengnan Zeng Pengli Guo Yuhan Zhang Xiaofeng Yang Jufang Jia Qinqin Zhang Beibei Zhang Bing Cao Ru Wang Xiaoke Zheng Weisheng Feng Effects of three types of fresh Rehmannia glutinosa improve lipopolysaccharide-induced acute kidney injury in sepsis through the estrogen receptor pathway Iranian Journal of Basic Medical Sciences acute kidney injury estrogen fresh rehmannia glutinosa lipopolysaccharide nf-kappa b nlrp3 sepsis tlr4 |
| title | Effects of three types of fresh Rehmannia glutinosa improve lipopolysaccharide-induced acute kidney injury in sepsis through the estrogen receptor pathway |
| title_full | Effects of three types of fresh Rehmannia glutinosa improve lipopolysaccharide-induced acute kidney injury in sepsis through the estrogen receptor pathway |
| title_fullStr | Effects of three types of fresh Rehmannia glutinosa improve lipopolysaccharide-induced acute kidney injury in sepsis through the estrogen receptor pathway |
| title_full_unstemmed | Effects of three types of fresh Rehmannia glutinosa improve lipopolysaccharide-induced acute kidney injury in sepsis through the estrogen receptor pathway |
| title_short | Effects of three types of fresh Rehmannia glutinosa improve lipopolysaccharide-induced acute kidney injury in sepsis through the estrogen receptor pathway |
| title_sort | effects of three types of fresh rehmannia glutinosa improve lipopolysaccharide induced acute kidney injury in sepsis through the estrogen receptor pathway |
| topic | acute kidney injury estrogen fresh rehmannia glutinosa lipopolysaccharide nf-kappa b nlrp3 sepsis tlr4 |
| url | https://ijbms.mums.ac.ir/article_21875_f0ea3eaed41fdefb514b3d00dd5d4f68.pdf |
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