Synthesis of Chalcones with Anticancer Activities
Several chalcones were synthesized and their<em> in vitro </em>cytotoxicity against various human cell lines, including human breast adenocarcinoma cell line MCF-7, human lung adenocarcinoma cell line A549, human prostate cancer cell line PC3, human adenocarcinoma cell li...
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| Format: | Article |
| Language: | English |
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MDPI AG
2012-05-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/17/6/6179 |
| _version_ | 1819231645492838400 |
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| author | Syam Mohan Siddig Ibrahim Abdelwahab Suvitha Syam Mohammed Ali Al-Mamary |
| author_facet | Syam Mohan Siddig Ibrahim Abdelwahab Suvitha Syam Mohammed Ali Al-Mamary |
| author_sort | Syam Mohan |
| collection | DOAJ |
| description | Several chalcones were synthesized and their<em> in vitro </em>cytotoxicity against various human cell lines, including human breast adenocarcinoma cell line MCF-7, human lung adenocarcinoma cell line A549, human prostate cancer cell line PC3, human adenocarcinoma cell line HT-29 (colorectal cancer) and human<strong> </strong>normal liver cell line WRL-68 was evaluated. Most of the compounds being active cytotoxic agents, four of them with minimal IC<sub>50</sub> values were chosen and studied in detail with MCF-7 cells. The compounds <strong>1</strong>, <strong>5</strong>, <strong>23</strong>, and <strong>25</strong> were capable in eliciting apoptosis in MCF-7 cells as shown by multiparameter cytotoxicity assay and caspase-3/7, -8, and -9 activities (<em>p</em> < 0.05). The ROS level showed 1.3-fold increase (<em>p</em> < 0.05) at the low concentrations used and thus it was concluded that the compounds increased the ROS level eventually leading to apoptosis in MCF-7 cells through intrinsic as well as extrinsic pathways. |
| first_indexed | 2024-12-23T11:48:15Z |
| format | Article |
| id | doaj.art-3091bc706ee54123881722a02172d480 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-23T11:48:15Z |
| publishDate | 2012-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-3091bc706ee54123881722a02172d4802022-12-21T17:48:17ZengMDPI AGMolecules1420-30492012-05-011766179619510.3390/molecules17066179Synthesis of Chalcones with Anticancer ActivitiesSyam MohanSiddig Ibrahim AbdelwahabSuvitha SyamMohammed Ali Al-MamarySeveral chalcones were synthesized and their<em> in vitro </em>cytotoxicity against various human cell lines, including human breast adenocarcinoma cell line MCF-7, human lung adenocarcinoma cell line A549, human prostate cancer cell line PC3, human adenocarcinoma cell line HT-29 (colorectal cancer) and human<strong> </strong>normal liver cell line WRL-68 was evaluated. Most of the compounds being active cytotoxic agents, four of them with minimal IC<sub>50</sub> values were chosen and studied in detail with MCF-7 cells. The compounds <strong>1</strong>, <strong>5</strong>, <strong>23</strong>, and <strong>25</strong> were capable in eliciting apoptosis in MCF-7 cells as shown by multiparameter cytotoxicity assay and caspase-3/7, -8, and -9 activities (<em>p</em> < 0.05). The ROS level showed 1.3-fold increase (<em>p</em> < 0.05) at the low concentrations used and thus it was concluded that the compounds increased the ROS level eventually leading to apoptosis in MCF-7 cells through intrinsic as well as extrinsic pathways.http://www.mdpi.com/1420-3049/17/6/6179chalconesyntheticcytotoxicityMCF-7 cellsapoptosishigh content screeningcaspaseROS |
| spellingShingle | Syam Mohan Siddig Ibrahim Abdelwahab Suvitha Syam Mohammed Ali Al-Mamary Synthesis of Chalcones with Anticancer Activities Molecules chalcone synthetic cytotoxicity MCF-7 cells apoptosis high content screening caspase ROS |
| title | Synthesis of Chalcones with Anticancer Activities |
| title_full | Synthesis of Chalcones with Anticancer Activities |
| title_fullStr | Synthesis of Chalcones with Anticancer Activities |
| title_full_unstemmed | Synthesis of Chalcones with Anticancer Activities |
| title_short | Synthesis of Chalcones with Anticancer Activities |
| title_sort | synthesis of chalcones with anticancer activities |
| topic | chalcone synthetic cytotoxicity MCF-7 cells apoptosis high content screening caspase ROS |
| url | http://www.mdpi.com/1420-3049/17/6/6179 |
| work_keys_str_mv | AT syammohan synthesisofchalconeswithanticanceractivities AT siddigibrahimabdelwahab synthesisofchalconeswithanticanceractivities AT suvithasyam synthesisofchalconeswithanticanceractivities AT mohammedalialmamary synthesisofchalconeswithanticanceractivities |