Trichothecene Mycotoxins Inhibit Mitochondrial Translation—Implication for the Mechanism of Toxicity
Fusarium head blight (FHB) reduces crop yield and results in contamination of grains with trichothecene mycotoxins. We previously showed that mitochondria play a critical role in the toxicity of a type B trichothecene. Here, we investigated the direct effects of type A and type B trichothecenes on m...
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| Format: | Article |
| Language: | English |
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MDPI AG
2011-12-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/3/12/1484/ |
| _version_ | 1811186406632980480 |
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| author | Susan McCormick Nilgun E. Tumer Debaleena Basu Mohamed Anwar Bin-Umer John E. McLaughlin |
| author_facet | Susan McCormick Nilgun E. Tumer Debaleena Basu Mohamed Anwar Bin-Umer John E. McLaughlin |
| author_sort | Susan McCormick |
| collection | DOAJ |
| description | Fusarium head blight (FHB) reduces crop yield and results in contamination of grains with trichothecene mycotoxins. We previously showed that mitochondria play a critical role in the toxicity of a type B trichothecene. Here, we investigated the direct effects of type A and type B trichothecenes on mitochondrial translation and membrane integrity in Saccharomyces cerevisiae. Sensitivity to trichothecenes increased when functional mitochondria were required for growth, and trichothecenes inhibited mitochondrial translation at concentrations, which did not inhibit total translation. In organello translation in isolated mitochondria was inhibited by type A and B trichothecenes, demonstrating that these toxins have a direct effect on mitochondrial translation. In intact yeast cells trichothecenes showed dose-dependent inhibition of mitochondrial membrane potential and reactive oxygen species, but only at doses higher than those affecting mitochondrial translation. These results demonstrate that inhibition of mitochondrial translation is a primary target of trichothecenes and is not secondary to the disruption of mitochondrial membranes. |
| first_indexed | 2024-04-11T13:46:02Z |
| format | Article |
| id | doaj.art-3091cd1310e043739e0de622e9dcb20b |
| institution | Directory Open Access Journal |
| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-11T13:46:02Z |
| publishDate | 2011-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Toxins |
| spelling | doaj.art-3091cd1310e043739e0de622e9dcb20b2022-12-22T04:21:05ZengMDPI AGToxins2072-66512011-12-013121484150110.3390/toxins3121484Trichothecene Mycotoxins Inhibit Mitochondrial Translation—Implication for the Mechanism of ToxicitySusan McCormickNilgun E. TumerDebaleena BasuMohamed Anwar Bin-UmerJohn E. McLaughlinFusarium head blight (FHB) reduces crop yield and results in contamination of grains with trichothecene mycotoxins. We previously showed that mitochondria play a critical role in the toxicity of a type B trichothecene. Here, we investigated the direct effects of type A and type B trichothecenes on mitochondrial translation and membrane integrity in Saccharomyces cerevisiae. Sensitivity to trichothecenes increased when functional mitochondria were required for growth, and trichothecenes inhibited mitochondrial translation at concentrations, which did not inhibit total translation. In organello translation in isolated mitochondria was inhibited by type A and B trichothecenes, demonstrating that these toxins have a direct effect on mitochondrial translation. In intact yeast cells trichothecenes showed dose-dependent inhibition of mitochondrial membrane potential and reactive oxygen species, but only at doses higher than those affecting mitochondrial translation. These results demonstrate that inhibition of mitochondrial translation is a primary target of trichothecenes and is not secondary to the disruption of mitochondrial membranes.http://www.mdpi.com/2072-6651/3/12/1484/trichothecenesdeoxynivalenolT-2 toxindiacetoxyscirpenolFusariummitochondriaribosomestranslationfusionFissionROS |
| spellingShingle | Susan McCormick Nilgun E. Tumer Debaleena Basu Mohamed Anwar Bin-Umer John E. McLaughlin Trichothecene Mycotoxins Inhibit Mitochondrial Translation—Implication for the Mechanism of Toxicity Toxins trichothecenes deoxynivalenol T-2 toxin diacetoxyscirpenol Fusarium mitochondria ribosomes translation fusion Fission ROS |
| title | Trichothecene Mycotoxins Inhibit Mitochondrial Translation—Implication for the Mechanism of Toxicity |
| title_full | Trichothecene Mycotoxins Inhibit Mitochondrial Translation—Implication for the Mechanism of Toxicity |
| title_fullStr | Trichothecene Mycotoxins Inhibit Mitochondrial Translation—Implication for the Mechanism of Toxicity |
| title_full_unstemmed | Trichothecene Mycotoxins Inhibit Mitochondrial Translation—Implication for the Mechanism of Toxicity |
| title_short | Trichothecene Mycotoxins Inhibit Mitochondrial Translation—Implication for the Mechanism of Toxicity |
| title_sort | trichothecene mycotoxins inhibit mitochondrial translation implication for the mechanism of toxicity |
| topic | trichothecenes deoxynivalenol T-2 toxin diacetoxyscirpenol Fusarium mitochondria ribosomes translation fusion Fission ROS |
| url | http://www.mdpi.com/2072-6651/3/12/1484/ |
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