Land use change alters phosphatase enzyme activity and phosphatase-harboring microbial abundance in the subalpine ecosystem of southeastern Qinghai-Tibet Plateau, China
In the presence of limited phosphorus (P) in terrestrial ecosystems, exploring how land use change (LUC) affects phosphatase enzyme activity and microbial communities is important for managing soil P, because microorganisms carry out the majority of P cycling in the soil through producing phosphatas...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Ecological Indicators |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1470160X23005587 |
| _version_ | 1827924593266917376 |
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| author | Belayneh Azene Renhuan Zhu Kaiwen Pan Xiaoming Sun Yalemzewd Nigussie Piotr Gruba Ali Raza Awoke Guadie Xiaogang Wu Lin Zhang |
| author_facet | Belayneh Azene Renhuan Zhu Kaiwen Pan Xiaoming Sun Yalemzewd Nigussie Piotr Gruba Ali Raza Awoke Guadie Xiaogang Wu Lin Zhang |
| author_sort | Belayneh Azene |
| collection | DOAJ |
| description | In the presence of limited phosphorus (P) in terrestrial ecosystems, exploring how land use change (LUC) affects phosphatase enzyme activity and microbial communities is important for managing soil P, because microorganisms carry out the majority of P cycling in the soil through producing phosphatase enzymes. In this study, we explored the impact of LUC on P availability, phosphatase enzyme, the abundance of phosphatase-encoding genes, and microorganisms. We collected 168 soil samples at soil depths of 0–20 cm and 20–40 cm from seven sampling sites, each of which represented by four different land uses: artificial forests (AF), farmlands (FL), natural forests (NF), and shrubland (SL). We analyzed phosphatase-encoding genes and microbes from metagenome datasets. Results indicated that P availability substantially increased following NF to FL conversion. In contrast, phosphatase enzyme activity significantly decreases when NF is converted to different land uses, due to the decline of soil organic carbon (SOC), moisture content (MC) and total nitrogen (TN). We have also detected 13 P solubilizing and mineralizing genes. The phoD and gcd were the dominant mineralizing and solubilizing genes, respectively. Farmland had higher gcd gene abundance, while NF had significantly higher phoD gene abundance. The gcd gene abundance were mainly governed by pH and total P, whereas pH and available P were the primary factors controlling phoD gene. MC, SOC and TN regulated other genes detected in this study. With regard to the dominant gcd-harboring phyla, Acidobacteria, Proteobacteria, Bacteroidetes and Gemmatimonadetes were the dominant, while Proteobacteria, Acidobacteria, Actinobacteria, and Candidatus_Rokubacteria were the dominant phoD-harboring phyla. The majority of gcd and phoD-harboring microorganisms were primarily controlled by pH, available P and total P. However, some phyla also regulated by MC, SOC, and TN. In general, our findings suggested that LUC significantly alters phosphatase enzymes and the abundance of phosphate-encoding genes and microbes. These changes have significant implications for soil P cycling. |
| first_indexed | 2024-03-13T05:12:50Z |
| format | Article |
| id | doaj.art-3094471b35014ce8bd675b38d4853056 |
| institution | Directory Open Access Journal |
| issn | 1470-160X |
| language | English |
| last_indexed | 2024-03-13T05:12:50Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecological Indicators |
| spelling | doaj.art-3094471b35014ce8bd675b38d48530562023-06-16T05:08:43ZengElsevierEcological Indicators1470-160X2023-09-01153110416Land use change alters phosphatase enzyme activity and phosphatase-harboring microbial abundance in the subalpine ecosystem of southeastern Qinghai-Tibet Plateau, ChinaBelayneh Azene0Renhuan Zhu1Kaiwen Pan2Xiaoming Sun3Yalemzewd Nigussie4Piotr Gruba5Ali Raza6Awoke Guadie7Xiaogang Wu8Lin Zhang9CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; University of Chinese Academy of Sciences, Beijing 100049, China; Ethiopian Forestry Development, Bahir Dar Center 2128, EthiopiaCAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaCAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; Corresponding authors.CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, ChinaStrayer University, College of Business, Virginia 2303 Dulles Station Blvd, Herndon, VA 20171, USADepartment of Forest Ecology and Silviculture, University of Agriculture, Al. 29 Listopada 46, Krakow 31-425, PolandCAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; University of Chinese Academy of Sciences, Beijing 100049, ChinaDepartment of Biology, College of Natural Sciences, Arba Minch University, Arba Minch 21, EthiopiaCAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, ChinaCAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; Corresponding authors.In the presence of limited phosphorus (P) in terrestrial ecosystems, exploring how land use change (LUC) affects phosphatase enzyme activity and microbial communities is important for managing soil P, because microorganisms carry out the majority of P cycling in the soil through producing phosphatase enzymes. In this study, we explored the impact of LUC on P availability, phosphatase enzyme, the abundance of phosphatase-encoding genes, and microorganisms. We collected 168 soil samples at soil depths of 0–20 cm and 20–40 cm from seven sampling sites, each of which represented by four different land uses: artificial forests (AF), farmlands (FL), natural forests (NF), and shrubland (SL). We analyzed phosphatase-encoding genes and microbes from metagenome datasets. Results indicated that P availability substantially increased following NF to FL conversion. In contrast, phosphatase enzyme activity significantly decreases when NF is converted to different land uses, due to the decline of soil organic carbon (SOC), moisture content (MC) and total nitrogen (TN). We have also detected 13 P solubilizing and mineralizing genes. The phoD and gcd were the dominant mineralizing and solubilizing genes, respectively. Farmland had higher gcd gene abundance, while NF had significantly higher phoD gene abundance. The gcd gene abundance were mainly governed by pH and total P, whereas pH and available P were the primary factors controlling phoD gene. MC, SOC and TN regulated other genes detected in this study. With regard to the dominant gcd-harboring phyla, Acidobacteria, Proteobacteria, Bacteroidetes and Gemmatimonadetes were the dominant, while Proteobacteria, Acidobacteria, Actinobacteria, and Candidatus_Rokubacteria were the dominant phoD-harboring phyla. The majority of gcd and phoD-harboring microorganisms were primarily controlled by pH, available P and total P. However, some phyla also regulated by MC, SOC, and TN. In general, our findings suggested that LUC significantly alters phosphatase enzymes and the abundance of phosphate-encoding genes and microbes. These changes have significant implications for soil P cycling.http://www.sciencedirect.com/science/article/pii/S1470160X23005587gcd-Encoding genegcd-Harboring microbesLand use changephoD-Encoding genephoD-Harboring microbesPhosphatase enzymes |
| spellingShingle | Belayneh Azene Renhuan Zhu Kaiwen Pan Xiaoming Sun Yalemzewd Nigussie Piotr Gruba Ali Raza Awoke Guadie Xiaogang Wu Lin Zhang Land use change alters phosphatase enzyme activity and phosphatase-harboring microbial abundance in the subalpine ecosystem of southeastern Qinghai-Tibet Plateau, China Ecological Indicators gcd-Encoding gene gcd-Harboring microbes Land use change phoD-Encoding gene phoD-Harboring microbes Phosphatase enzymes |
| title | Land use change alters phosphatase enzyme activity and phosphatase-harboring microbial abundance in the subalpine ecosystem of southeastern Qinghai-Tibet Plateau, China |
| title_full | Land use change alters phosphatase enzyme activity and phosphatase-harboring microbial abundance in the subalpine ecosystem of southeastern Qinghai-Tibet Plateau, China |
| title_fullStr | Land use change alters phosphatase enzyme activity and phosphatase-harboring microbial abundance in the subalpine ecosystem of southeastern Qinghai-Tibet Plateau, China |
| title_full_unstemmed | Land use change alters phosphatase enzyme activity and phosphatase-harboring microbial abundance in the subalpine ecosystem of southeastern Qinghai-Tibet Plateau, China |
| title_short | Land use change alters phosphatase enzyme activity and phosphatase-harboring microbial abundance in the subalpine ecosystem of southeastern Qinghai-Tibet Plateau, China |
| title_sort | land use change alters phosphatase enzyme activity and phosphatase harboring microbial abundance in the subalpine ecosystem of southeastern qinghai tibet plateau china |
| topic | gcd-Encoding gene gcd-Harboring microbes Land use change phoD-Encoding gene phoD-Harboring microbes Phosphatase enzymes |
| url | http://www.sciencedirect.com/science/article/pii/S1470160X23005587 |
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