Chlorogenic Acid Protects Against Indomethacin-Induced Inflammation and Mucosa Damage by Decreasing Bacteroides-Derived LPS
Background: Chlorogenic acid (CGA), a natural bioactive polyphenol, exerts anti-inflammatory, antioxidant, and antibacterial effects that support the maintenance of intestinal health. However, the influence of CGA on gut microbiota and their metabolites, as well as its potential effects and mechanis...
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| Format: | Article |
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Frontiers Media S.A.
2020-06-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2020.01125/full |
| _version_ | 1828825275454980096 |
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| author | Yongwang Yan Yongwang Yan Xu Zhou Kangxiao Guo Feng Zhou Hongqi Yang |
| author_facet | Yongwang Yan Yongwang Yan Xu Zhou Kangxiao Guo Feng Zhou Hongqi Yang |
| author_sort | Yongwang Yan |
| collection | DOAJ |
| description | Background: Chlorogenic acid (CGA), a natural bioactive polyphenol, exerts anti-inflammatory, antioxidant, and antibacterial effects that support the maintenance of intestinal health. However, the influence of CGA on gut microbiota and their metabolites, as well as its potential effects and mechanism of action in inflammatory bowel disease, remain to be elucidated.Methods: First, an oral gavage was used to administer CGA to indomethacin-treated mice. Then, fecal microbiota transplantation was performed to explore the role of intestinal microbiota in indomethacin-induced inflammation.Results: CGA treatment protected against body weight loss, damage to intestinal morphology and integrity, inflammation, and alteration of microbiota composition in indomethacin-treated mice. Interestingly, CGA failed to inhibit inflammation or protect intestine integrity in mice treated with antibiotics. Notably, mice who had been colonized with intestinal microbiota from CGA-treated or CGA-and-indomethacin-treated mice, through the fecal microbiota transplantation program, were protected from indomethacin-induced inflammation, growth of Bacteroides, and the accumulation of Bacteroides-derived LPS, in congruence with those who had been treated with CGA.Conclusion: The results suggest that CGA may protect intestine integrity and alleviate inflammatory responses, primarily by inhibiting the growth of Bacteroides and the accumulation of Bacteroides-derived LPS, in indomethacin-induced colitis. This newly identified mechanism broadens our knowledge of how CGA exerts protective effects on intestinal inflammation and provides strategies for the prevention of gastrointestinal mucosal damage in patients treated with indomethacin. |
| first_indexed | 2024-12-12T14:12:52Z |
| format | Article |
| id | doaj.art-3095c0164999429c95752667218cc5ed |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-12T14:12:52Z |
| publishDate | 2020-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-3095c0164999429c95752667218cc5ed2022-12-22T00:21:59ZengFrontiers Media S.A.Frontiers in Immunology1664-32242020-06-011110.3389/fimmu.2020.01125544504Chlorogenic Acid Protects Against Indomethacin-Induced Inflammation and Mucosa Damage by Decreasing Bacteroides-Derived LPSYongwang Yan0Yongwang Yan1Xu Zhou2Kangxiao Guo3Feng Zhou4Hongqi Yang5College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, ChinaPharmaceutical College, Changsha Health Vocational College, Changsha, ChinaDepartment of Gastroenterology, Changsha Hospital of Traditional Chinese Medicine, Changsha, ChinaPharmaceutical College, Changsha Health Vocational College, Changsha, ChinaPharmaceutical College, Changsha Health Vocational College, Changsha, ChinaCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, ChinaBackground: Chlorogenic acid (CGA), a natural bioactive polyphenol, exerts anti-inflammatory, antioxidant, and antibacterial effects that support the maintenance of intestinal health. However, the influence of CGA on gut microbiota and their metabolites, as well as its potential effects and mechanism of action in inflammatory bowel disease, remain to be elucidated.Methods: First, an oral gavage was used to administer CGA to indomethacin-treated mice. Then, fecal microbiota transplantation was performed to explore the role of intestinal microbiota in indomethacin-induced inflammation.Results: CGA treatment protected against body weight loss, damage to intestinal morphology and integrity, inflammation, and alteration of microbiota composition in indomethacin-treated mice. Interestingly, CGA failed to inhibit inflammation or protect intestine integrity in mice treated with antibiotics. Notably, mice who had been colonized with intestinal microbiota from CGA-treated or CGA-and-indomethacin-treated mice, through the fecal microbiota transplantation program, were protected from indomethacin-induced inflammation, growth of Bacteroides, and the accumulation of Bacteroides-derived LPS, in congruence with those who had been treated with CGA.Conclusion: The results suggest that CGA may protect intestine integrity and alleviate inflammatory responses, primarily by inhibiting the growth of Bacteroides and the accumulation of Bacteroides-derived LPS, in indomethacin-induced colitis. This newly identified mechanism broadens our knowledge of how CGA exerts protective effects on intestinal inflammation and provides strategies for the prevention of gastrointestinal mucosal damage in patients treated with indomethacin.https://www.frontiersin.org/article/10.3389/fimmu.2020.01125/fullchlorogenic acidindomethacininflammatory bowel diseaseintestinal integritymicrobiota |
| spellingShingle | Yongwang Yan Yongwang Yan Xu Zhou Kangxiao Guo Feng Zhou Hongqi Yang Chlorogenic Acid Protects Against Indomethacin-Induced Inflammation and Mucosa Damage by Decreasing Bacteroides-Derived LPS Frontiers in Immunology chlorogenic acid indomethacin inflammatory bowel disease intestinal integrity microbiota |
| title | Chlorogenic Acid Protects Against Indomethacin-Induced Inflammation and Mucosa Damage by Decreasing Bacteroides-Derived LPS |
| title_full | Chlorogenic Acid Protects Against Indomethacin-Induced Inflammation and Mucosa Damage by Decreasing Bacteroides-Derived LPS |
| title_fullStr | Chlorogenic Acid Protects Against Indomethacin-Induced Inflammation and Mucosa Damage by Decreasing Bacteroides-Derived LPS |
| title_full_unstemmed | Chlorogenic Acid Protects Against Indomethacin-Induced Inflammation and Mucosa Damage by Decreasing Bacteroides-Derived LPS |
| title_short | Chlorogenic Acid Protects Against Indomethacin-Induced Inflammation and Mucosa Damage by Decreasing Bacteroides-Derived LPS |
| title_sort | chlorogenic acid protects against indomethacin induced inflammation and mucosa damage by decreasing bacteroides derived lps |
| topic | chlorogenic acid indomethacin inflammatory bowel disease intestinal integrity microbiota |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2020.01125/full |
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