Association of the Gelatinase B/Metalloproteinase 9 (MMP-9) Gene Haplotype in Systemic Lupus Erythematosus (SLE) in the Pediatric Egyptian Population
Permanent systemic inflammation is a defining feature of systemic lupus erythematosus (SLE), which affects multiple organs. Gelatinase B/matrix metalloproteinase-9 (MMP-9) is an essential protease investigated in inflammation that has been linked to SLE. The study’s objective was to investigate the...
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MDPI AG
2022-08-01
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| author | Zeinab R. Attia Mohamed M. Zedan Thuraya M. Mutawi Entsar A. Saad Rania A. Abd El Azeem Mohamed A. El Basuni |
| author_facet | Zeinab R. Attia Mohamed M. Zedan Thuraya M. Mutawi Entsar A. Saad Rania A. Abd El Azeem Mohamed A. El Basuni |
| author_sort | Zeinab R. Attia |
| collection | DOAJ |
| description | Permanent systemic inflammation is a defining feature of systemic lupus erythematosus (SLE), which affects multiple organs. Gelatinase B/matrix metalloproteinase-9 (MMP-9) is an essential protease investigated in inflammation that has been linked to SLE. The study’s objective was to investigate the relationship between the rs3918249 T/C and rs17576 A/G SNPs in the MMP-9 gene with SLE. The study was conducted with 100 SLE cases and 100 age/sex-matched healthy individuals. TaqMan<sup>TM</sup> SNP was used for genotyping by real time PCR on the Artus Rotor-Gene Qiagen equipment. Haplotypes (TG: OR = 0.226, 95% CI = 0.119–0.429) and (CA: OR = 0.36, 95% CI = 0.2206–0.631), both with a <i>p</i>-value < 0.001 were substantially linked to a lower incidence of SLE. Conversely, the risk of SLE was not associated with the individual SNPs studied. The haplotype analysis was more significant than the SNP analysis and may correlate with the decreased risk of SLE in children and adolescents in Egypt. |
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| issn | 2227-9067 |
| language | English |
| last_indexed | 2024-03-10T00:24:33Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-3095f4b2c5c143c899a97fb87f9206f42023-11-23T15:36:10ZengMDPI AGChildren2227-90672022-08-0199127110.3390/children9091271Association of the Gelatinase B/Metalloproteinase 9 (MMP-9) Gene Haplotype in Systemic Lupus Erythematosus (SLE) in the Pediatric Egyptian PopulationZeinab R. Attia0Mohamed M. Zedan1Thuraya M. Mutawi2Entsar A. Saad3Rania A. Abd El Azeem4Mohamed A. El Basuni5Mansoura University Children’s Hospital, Mansoura University, Mansoura 35516, EgyptDepartment of Pediatrics, Faculty of Medicine, Mansoura University, Mansoura 35516, EgyptMansoura University Children’s Hospital, Mansoura University, Mansoura 35516, EgyptChemistry Department, Faculty of Science, Damietta University, Damietta 34511, EgyptMansoura University Children’s Hospital, Mansoura University, Mansoura 35516, EgyptMansoura University Children’s Hospital, Mansoura University, Mansoura 35516, EgyptPermanent systemic inflammation is a defining feature of systemic lupus erythematosus (SLE), which affects multiple organs. Gelatinase B/matrix metalloproteinase-9 (MMP-9) is an essential protease investigated in inflammation that has been linked to SLE. The study’s objective was to investigate the relationship between the rs3918249 T/C and rs17576 A/G SNPs in the MMP-9 gene with SLE. The study was conducted with 100 SLE cases and 100 age/sex-matched healthy individuals. TaqMan<sup>TM</sup> SNP was used for genotyping by real time PCR on the Artus Rotor-Gene Qiagen equipment. Haplotypes (TG: OR = 0.226, 95% CI = 0.119–0.429) and (CA: OR = 0.36, 95% CI = 0.2206–0.631), both with a <i>p</i>-value < 0.001 were substantially linked to a lower incidence of SLE. Conversely, the risk of SLE was not associated with the individual SNPs studied. The haplotype analysis was more significant than the SNP analysis and may correlate with the decreased risk of SLE in children and adolescents in Egypt.https://www.mdpi.com/2227-9067/9/9/1271SNPshaplotypesinflammationmatrix metalloproteinase-9SLE riskgenotyping |
| spellingShingle | Zeinab R. Attia Mohamed M. Zedan Thuraya M. Mutawi Entsar A. Saad Rania A. Abd El Azeem Mohamed A. El Basuni Association of the Gelatinase B/Metalloproteinase 9 (MMP-9) Gene Haplotype in Systemic Lupus Erythematosus (SLE) in the Pediatric Egyptian Population Children SNPs haplotypes inflammation matrix metalloproteinase-9 SLE risk genotyping |
| title | Association of the Gelatinase B/Metalloproteinase 9 (MMP-9) Gene Haplotype in Systemic Lupus Erythematosus (SLE) in the Pediatric Egyptian Population |
| title_full | Association of the Gelatinase B/Metalloproteinase 9 (MMP-9) Gene Haplotype in Systemic Lupus Erythematosus (SLE) in the Pediatric Egyptian Population |
| title_fullStr | Association of the Gelatinase B/Metalloproteinase 9 (MMP-9) Gene Haplotype in Systemic Lupus Erythematosus (SLE) in the Pediatric Egyptian Population |
| title_full_unstemmed | Association of the Gelatinase B/Metalloproteinase 9 (MMP-9) Gene Haplotype in Systemic Lupus Erythematosus (SLE) in the Pediatric Egyptian Population |
| title_short | Association of the Gelatinase B/Metalloproteinase 9 (MMP-9) Gene Haplotype in Systemic Lupus Erythematosus (SLE) in the Pediatric Egyptian Population |
| title_sort | association of the gelatinase b metalloproteinase 9 mmp 9 gene haplotype in systemic lupus erythematosus sle in the pediatric egyptian population |
| topic | SNPs haplotypes inflammation matrix metalloproteinase-9 SLE risk genotyping |
| url | https://www.mdpi.com/2227-9067/9/9/1271 |
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