High Antioxidant Capacity of <i>Lacticaseibacillus paracasei</i> TDM-2 and <i>Pediococcus pentosaceus</i> TCM-3 from Qinghai Tibetan Plateau and Their Function towards Gut Modulation
Probiotic supplementation is a key therapeutic strategy for promoting gut health and maintaining gut homeostasis by modulating functional microbiota. In this study, we isolated two lactic acid bacteria (LAB) strains, <i>Pediococcus pentosaceus</i> TCM-3 and <i>Lacticaseibacillus pa...
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MDPI AG
2023-04-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/9/1814 |
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| author | Tingyu Yang Xueni Fan Diantong Li Tingting Zhao Dan Wu Zhenjiang Liu Danfeng Long Bin Li Xiaodan Huang |
| author_facet | Tingyu Yang Xueni Fan Diantong Li Tingting Zhao Dan Wu Zhenjiang Liu Danfeng Long Bin Li Xiaodan Huang |
| author_sort | Tingyu Yang |
| collection | DOAJ |
| description | Probiotic supplementation is a key therapeutic strategy for promoting gut health and maintaining gut homeostasis by modulating functional microbiota. In this study, we isolated two lactic acid bacteria (LAB) strains, <i>Pediococcus pentosaceus</i> TCM-3 and <i>Lacticaseibacillus paracasei</i> TDM-2, from Qinghai-Tibetan plateau, and evaluated their probiotic properties and antioxidant bioactivity. In which, TDM-2 had higher T-AOC activity than either TCM-3 or LGG (4.10 μmol/mL vs. 3.68 and 3.53 μmol/mL, respectively, <i>p</i> < 0.05). These strains have shown high antioxidant activity compared to the LAB strains and were found to be acid and bile salt tolerant, confronting the safety issues of antibiotic resistance and the capability of surviving in simulated gastric and intestinal juices. In vitro fermentation experiments with human gut microbiota revealed significant differences in microbial community composition between samples supplemented with TCM-3 and TDM-2 and those without. The addition of these two strains resulted in an enrichment of beneficial taxa, such as the <i>Pediococcus</i>, <i>Lactobacillus</i>, and <i>Clostridium_sensu_strictos</i> at the genus level, and Firmicutes and Proteobacteria at the phylum level. Notably, the TCM-3 group exhibited higher short-chain fatty acid production than the TDM-2 group and untreated controls (acetic acid at 12 h: 4.54 mmol L<sup>−1</sup> vs. 4.06 mmol L<sup>−1</sup> and 4.00 mmol L<sup>−1</sup>; acetic acid at 24 h: 4.99 mmol L<sup>−1</sup> vs. 4.90 mmol L<sup>−1</sup> and 4.82 mmol L<sup>−1</sup>, <i>p</i> < 0.05). These findings demonstrate that LAB supplementation with high antioxidant capacity and probiotic properties can promote gut health by modulating functional microbiota and is enriching for beneficial taxa. Our study provides guidance for therapeutic strategies that use novel LAB strains to maintain gut homeostasis and functional microbiota modulation. |
| first_indexed | 2024-03-11T04:18:59Z |
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| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T04:18:59Z |
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| spelling | doaj.art-309a460a2c64495f95f9cfeeca8717c82023-11-17T22:55:16ZengMDPI AGFoods2304-81582023-04-01129181410.3390/foods12091814High Antioxidant Capacity of <i>Lacticaseibacillus paracasei</i> TDM-2 and <i>Pediococcus pentosaceus</i> TCM-3 from Qinghai Tibetan Plateau and Their Function towards Gut ModulationTingyu Yang0Xueni Fan1Diantong Li2Tingting Zhao3Dan Wu4Zhenjiang Liu5Danfeng Long6Bin Li7Xiaodan Huang8School of Public Health, Lanzhou University, Lanzhou 730033, ChinaSchool of Public Health, Lanzhou University, Lanzhou 730033, ChinaSchool of Public Health, Lanzhou University, Lanzhou 730033, ChinaSchool of Public Health, Lanzhou University, Lanzhou 730033, ChinaInstitute of Animal Husbandry and Veterinary, Tibet Academy of Agricultural and Animal Husbandry Sciences, Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lhasa 850000, ChinaNational Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun 130012, ChinaSchool of Public Health, Lanzhou University, Lanzhou 730033, ChinaInstitute of Animal Husbandry and Veterinary, Tibet Academy of Agricultural and Animal Husbandry Sciences, Key Laboratory of Animal Genetics and Breeding on Tibetan Plateau, Ministry of Agriculture and Rural Affairs, Lhasa 850000, ChinaSchool of Public Health, Lanzhou University, Lanzhou 730033, ChinaProbiotic supplementation is a key therapeutic strategy for promoting gut health and maintaining gut homeostasis by modulating functional microbiota. In this study, we isolated two lactic acid bacteria (LAB) strains, <i>Pediococcus pentosaceus</i> TCM-3 and <i>Lacticaseibacillus paracasei</i> TDM-2, from Qinghai-Tibetan plateau, and evaluated their probiotic properties and antioxidant bioactivity. In which, TDM-2 had higher T-AOC activity than either TCM-3 or LGG (4.10 μmol/mL vs. 3.68 and 3.53 μmol/mL, respectively, <i>p</i> < 0.05). These strains have shown high antioxidant activity compared to the LAB strains and were found to be acid and bile salt tolerant, confronting the safety issues of antibiotic resistance and the capability of surviving in simulated gastric and intestinal juices. In vitro fermentation experiments with human gut microbiota revealed significant differences in microbial community composition between samples supplemented with TCM-3 and TDM-2 and those without. The addition of these two strains resulted in an enrichment of beneficial taxa, such as the <i>Pediococcus</i>, <i>Lactobacillus</i>, and <i>Clostridium_sensu_strictos</i> at the genus level, and Firmicutes and Proteobacteria at the phylum level. Notably, the TCM-3 group exhibited higher short-chain fatty acid production than the TDM-2 group and untreated controls (acetic acid at 12 h: 4.54 mmol L<sup>−1</sup> vs. 4.06 mmol L<sup>−1</sup> and 4.00 mmol L<sup>−1</sup>; acetic acid at 24 h: 4.99 mmol L<sup>−1</sup> vs. 4.90 mmol L<sup>−1</sup> and 4.82 mmol L<sup>−1</sup>, <i>p</i> < 0.05). These findings demonstrate that LAB supplementation with high antioxidant capacity and probiotic properties can promote gut health by modulating functional microbiota and is enriching for beneficial taxa. Our study provides guidance for therapeutic strategies that use novel LAB strains to maintain gut homeostasis and functional microbiota modulation.https://www.mdpi.com/2304-8158/12/9/1814lactic acid bacteriaQinghai-Tibetan plateauantioxidant capacitygut microbiota |
| spellingShingle | Tingyu Yang Xueni Fan Diantong Li Tingting Zhao Dan Wu Zhenjiang Liu Danfeng Long Bin Li Xiaodan Huang High Antioxidant Capacity of <i>Lacticaseibacillus paracasei</i> TDM-2 and <i>Pediococcus pentosaceus</i> TCM-3 from Qinghai Tibetan Plateau and Their Function towards Gut Modulation Foods lactic acid bacteria Qinghai-Tibetan plateau antioxidant capacity gut microbiota |
| title | High Antioxidant Capacity of <i>Lacticaseibacillus paracasei</i> TDM-2 and <i>Pediococcus pentosaceus</i> TCM-3 from Qinghai Tibetan Plateau and Their Function towards Gut Modulation |
| title_full | High Antioxidant Capacity of <i>Lacticaseibacillus paracasei</i> TDM-2 and <i>Pediococcus pentosaceus</i> TCM-3 from Qinghai Tibetan Plateau and Their Function towards Gut Modulation |
| title_fullStr | High Antioxidant Capacity of <i>Lacticaseibacillus paracasei</i> TDM-2 and <i>Pediococcus pentosaceus</i> TCM-3 from Qinghai Tibetan Plateau and Their Function towards Gut Modulation |
| title_full_unstemmed | High Antioxidant Capacity of <i>Lacticaseibacillus paracasei</i> TDM-2 and <i>Pediococcus pentosaceus</i> TCM-3 from Qinghai Tibetan Plateau and Their Function towards Gut Modulation |
| title_short | High Antioxidant Capacity of <i>Lacticaseibacillus paracasei</i> TDM-2 and <i>Pediococcus pentosaceus</i> TCM-3 from Qinghai Tibetan Plateau and Their Function towards Gut Modulation |
| title_sort | high antioxidant capacity of i lacticaseibacillus paracasei i tdm 2 and i pediococcus pentosaceus i tcm 3 from qinghai tibetan plateau and their function towards gut modulation |
| topic | lactic acid bacteria Qinghai-Tibetan plateau antioxidant capacity gut microbiota |
| url | https://www.mdpi.com/2304-8158/12/9/1814 |
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