Anti-Inflammatory Effect of <i>Turbo cornutus</i> Viscera Ethanolic Extract against Lipopolysaccharide-Stimulated Inflammatory Response via the Regulation of the JNK/NF-kB Signaling Pathway in Murine Macrophage RAW 264.7 Cells and a Zebrafish Model: A Preliminary Study
<i>Turbo cornutus</i>, the horned turban sea snail, is found along the intertidal and basaltic shorelines and is an important fishery resource of Jeju Island. In this study, we performed a preliminary study on anti-inflammatory effect of 70% ethanol extract obtained from <i>T. corn...
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| Format: | Article |
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MDPI AG
2022-01-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/3/364 |
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| author | Eun-A Kim Nalae Kang Junseong Kim Hye-Won Yang Ginnae Ahn Soo-Jin Heo |
| author_facet | Eun-A Kim Nalae Kang Junseong Kim Hye-Won Yang Ginnae Ahn Soo-Jin Heo |
| author_sort | Eun-A Kim |
| collection | DOAJ |
| description | <i>Turbo cornutus</i>, the horned turban sea snail, is found along the intertidal and basaltic shorelines and is an important fishery resource of Jeju Island. In this study, we performed a preliminary study on anti-inflammatory effect of 70% ethanol extract obtained from <i>T. cornutus</i> viscera (TVE) on lipopolysaccharide (LPS)-stimulated RAW264.7 cells in vitro and zebrafish embryos in vivo. TVE reduced the production of LPS-stimulated nitric oxide (NO) and prostaglandin E2 (PGE2) without any toxic effects. TVE also decreased the protein expression of LPS-induced inducible NO synthase and cyclooxygenase-2 and suppressed the production of pro-inflammatory cytokines, including tumor necrosis factor-α, interleukin (IL)-6, and IL-1β. Furthermore, mechanistic studies indicated that TVE suppressed c-Jun N-terminal kinase phosphorylation and nuclear factor-kB activation. In zebrafish embryos, TVE did not show developmental toxicity based on the survival rate and cell death findings. In LPS-stimulated zebrafish embryos, TVE suppressed NO production and cell death. In conclusion, the result from this preliminary study showed TVE has a potential anti-inflammatory property that can be exploited as a functional food ingredient. |
| first_indexed | 2024-03-09T23:53:19Z |
| format | Article |
| id | doaj.art-309b20ee8d0f4250854f1b18899b13c8 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T23:53:19Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-309b20ee8d0f4250854f1b18899b13c82023-11-23T16:29:41ZengMDPI AGFoods2304-81582022-01-0111336410.3390/foods11030364Anti-Inflammatory Effect of <i>Turbo cornutus</i> Viscera Ethanolic Extract against Lipopolysaccharide-Stimulated Inflammatory Response via the Regulation of the JNK/NF-kB Signaling Pathway in Murine Macrophage RAW 264.7 Cells and a Zebrafish Model: A Preliminary StudyEun-A Kim0Nalae Kang1Junseong Kim2Hye-Won Yang3Ginnae Ahn4Soo-Jin Heo5Jeju Marine Research Center, Korea Institute of Ocean Science & Technology (KIOST), Jeju 63349, KoreaJeju Marine Research Center, Korea Institute of Ocean Science & Technology (KIOST), Jeju 63349, KoreaJeju Marine Research Center, Korea Institute of Ocean Science & Technology (KIOST), Jeju 63349, KoreaDepartment of Marine Life Sciences, Jeju National University, Jeju 63243, KoreaDepartment of Food Technology and Nutrition, Chonnam National University, Yeosu 59626, KoreaJeju Marine Research Center, Korea Institute of Ocean Science & Technology (KIOST), Jeju 63349, Korea<i>Turbo cornutus</i>, the horned turban sea snail, is found along the intertidal and basaltic shorelines and is an important fishery resource of Jeju Island. In this study, we performed a preliminary study on anti-inflammatory effect of 70% ethanol extract obtained from <i>T. cornutus</i> viscera (TVE) on lipopolysaccharide (LPS)-stimulated RAW264.7 cells in vitro and zebrafish embryos in vivo. TVE reduced the production of LPS-stimulated nitric oxide (NO) and prostaglandin E2 (PGE2) without any toxic effects. TVE also decreased the protein expression of LPS-induced inducible NO synthase and cyclooxygenase-2 and suppressed the production of pro-inflammatory cytokines, including tumor necrosis factor-α, interleukin (IL)-6, and IL-1β. Furthermore, mechanistic studies indicated that TVE suppressed c-Jun N-terminal kinase phosphorylation and nuclear factor-kB activation. In zebrafish embryos, TVE did not show developmental toxicity based on the survival rate and cell death findings. In LPS-stimulated zebrafish embryos, TVE suppressed NO production and cell death. In conclusion, the result from this preliminary study showed TVE has a potential anti-inflammatory property that can be exploited as a functional food ingredient.https://www.mdpi.com/2304-8158/11/3/364<i>Turbo cornutus</i>visceraanti-inflammatoryRAW264.7 cellszebrafish |
| spellingShingle | Eun-A Kim Nalae Kang Junseong Kim Hye-Won Yang Ginnae Ahn Soo-Jin Heo Anti-Inflammatory Effect of <i>Turbo cornutus</i> Viscera Ethanolic Extract against Lipopolysaccharide-Stimulated Inflammatory Response via the Regulation of the JNK/NF-kB Signaling Pathway in Murine Macrophage RAW 264.7 Cells and a Zebrafish Model: A Preliminary Study Foods <i>Turbo cornutus</i> viscera anti-inflammatory RAW264.7 cells zebrafish |
| title | Anti-Inflammatory Effect of <i>Turbo cornutus</i> Viscera Ethanolic Extract against Lipopolysaccharide-Stimulated Inflammatory Response via the Regulation of the JNK/NF-kB Signaling Pathway in Murine Macrophage RAW 264.7 Cells and a Zebrafish Model: A Preliminary Study |
| title_full | Anti-Inflammatory Effect of <i>Turbo cornutus</i> Viscera Ethanolic Extract against Lipopolysaccharide-Stimulated Inflammatory Response via the Regulation of the JNK/NF-kB Signaling Pathway in Murine Macrophage RAW 264.7 Cells and a Zebrafish Model: A Preliminary Study |
| title_fullStr | Anti-Inflammatory Effect of <i>Turbo cornutus</i> Viscera Ethanolic Extract against Lipopolysaccharide-Stimulated Inflammatory Response via the Regulation of the JNK/NF-kB Signaling Pathway in Murine Macrophage RAW 264.7 Cells and a Zebrafish Model: A Preliminary Study |
| title_full_unstemmed | Anti-Inflammatory Effect of <i>Turbo cornutus</i> Viscera Ethanolic Extract against Lipopolysaccharide-Stimulated Inflammatory Response via the Regulation of the JNK/NF-kB Signaling Pathway in Murine Macrophage RAW 264.7 Cells and a Zebrafish Model: A Preliminary Study |
| title_short | Anti-Inflammatory Effect of <i>Turbo cornutus</i> Viscera Ethanolic Extract against Lipopolysaccharide-Stimulated Inflammatory Response via the Regulation of the JNK/NF-kB Signaling Pathway in Murine Macrophage RAW 264.7 Cells and a Zebrafish Model: A Preliminary Study |
| title_sort | anti inflammatory effect of i turbo cornutus i viscera ethanolic extract against lipopolysaccharide stimulated inflammatory response via the regulation of the jnk nf kb signaling pathway in murine macrophage raw 264 7 cells and a zebrafish model a preliminary study |
| topic | <i>Turbo cornutus</i> viscera anti-inflammatory RAW264.7 cells zebrafish |
| url | https://www.mdpi.com/2304-8158/11/3/364 |
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