Rapid Diagnosis of HIV-1 virus by Near Infrared Spectroscopy: based on Partial least squares regression
Currently, the laboratory diagnostic tests available for HIV-1 viral infection are mainly based on serological testing which relies on enzyme-linked immunosorbent assay (ELISA) for blood HIV antigen detection and reverse transcription polymerase chain reaction (RT-PCR) for HIV specific RNA sequence...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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EDP Sciences
2021-01-01
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| Series: | E3S Web of Conferences |
| Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2021/47/e3sconf_icepe2021_03067.pdf |
| _version_ | 1819259905274544128 |
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| author | He Xiaohong Song Zhihong Shang Haifei Yang Silang Wu Lujing Ma Yan Sun Yu Xia Xuejiao Wang Quangui Luo Bei Gu Chaojiang |
| author_facet | He Xiaohong Song Zhihong Shang Haifei Yang Silang Wu Lujing Ma Yan Sun Yu Xia Xuejiao Wang Quangui Luo Bei Gu Chaojiang |
| author_sort | He Xiaohong |
| collection | DOAJ |
| description | Currently, the laboratory diagnostic tests available for HIV-1 viral infection are mainly based on serological testing which relies on enzyme-linked immunosorbent assay (ELISA) for blood HIV antigen detection and reverse transcription polymerase chain reaction (RT-PCR) for HIV specific RNA sequence identification. However, these methods are expensive and time-consuming, and suffer from false positive and/or false negative results. Thus, there is an urgent need for developing a cost effective, rapid and accurate diagnostic method for HIV-1 infection. In order to reduce the barriers for effective diagnosis, a near-infrared spectroscopy (NIR) method was used to detect the HIV-1 virus in human serum, specifically, three absorption peaks with dose-dependent at 1582nm, 1810nm and 2363nm were found by multiple FBiPLSR test analysis for HIV-nano and HIV-EGFP, but not for MLV. Therefore, we recommend the use of 1582nm, 1810nm and 2363nm as the characteristic spectrum peak, for early screening and rapid diagnosis of serum HIV. |
| first_indexed | 2024-12-23T19:17:26Z |
| format | Article |
| id | doaj.art-309b599b597f4c3ab8d2297ff54e1b18 |
| institution | Directory Open Access Journal |
| issn | 2267-1242 |
| language | English |
| last_indexed | 2024-12-23T19:17:26Z |
| publishDate | 2021-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj.art-309b599b597f4c3ab8d2297ff54e1b182022-12-21T17:34:16ZengEDP SciencesE3S Web of Conferences2267-12422021-01-012710306710.1051/e3sconf/202127103067e3sconf_icepe2021_03067Rapid Diagnosis of HIV-1 virus by Near Infrared Spectroscopy: based on Partial least squares regressionHe Xiaohong0Song Zhihong1Shang Haifei2Yang Silang3Wu Lujing4Ma Yan5Sun Yu6Xia Xuejiao7Wang Quangui8Luo Bei9Gu Chaojiang10College of Life Science and Health, Wuhan University of Science and TechnologyCollege of Life Science and Health, Wuhan University of Science and TechnologyCollege of Life Science and Health, Wuhan University of Science and TechnologyCollege of Life Science and Health, Wuhan University of Science and TechnologyCollege of Life Science and Health, Wuhan University of Science and TechnologyCollege of Life Science and Health, Wuhan University of Science and TechnologyCollege of Life Science and Health, Wuhan University of Science and TechnologyCollege of Life Science and Health, Wuhan University of Science and TechnologyCollege of Information Science and Engineering, Hunan UniversityWuhan Qiangu Technology Co., Ltd.College of Life Science and Health, Wuhan University of Science and TechnologyCurrently, the laboratory diagnostic tests available for HIV-1 viral infection are mainly based on serological testing which relies on enzyme-linked immunosorbent assay (ELISA) for blood HIV antigen detection and reverse transcription polymerase chain reaction (RT-PCR) for HIV specific RNA sequence identification. However, these methods are expensive and time-consuming, and suffer from false positive and/or false negative results. Thus, there is an urgent need for developing a cost effective, rapid and accurate diagnostic method for HIV-1 infection. In order to reduce the barriers for effective diagnosis, a near-infrared spectroscopy (NIR) method was used to detect the HIV-1 virus in human serum, specifically, three absorption peaks with dose-dependent at 1582nm, 1810nm and 2363nm were found by multiple FBiPLSR test analysis for HIV-nano and HIV-EGFP, but not for MLV. Therefore, we recommend the use of 1582nm, 1810nm and 2363nm as the characteristic spectrum peak, for early screening and rapid diagnosis of serum HIV.https://www.e3s-conferences.org/articles/e3sconf/pdf/2021/47/e3sconf_icepe2021_03067.pdf |
| spellingShingle | He Xiaohong Song Zhihong Shang Haifei Yang Silang Wu Lujing Ma Yan Sun Yu Xia Xuejiao Wang Quangui Luo Bei Gu Chaojiang Rapid Diagnosis of HIV-1 virus by Near Infrared Spectroscopy: based on Partial least squares regression E3S Web of Conferences |
| title | Rapid Diagnosis of HIV-1 virus by Near Infrared Spectroscopy: based on Partial least squares regression |
| title_full | Rapid Diagnosis of HIV-1 virus by Near Infrared Spectroscopy: based on Partial least squares regression |
| title_fullStr | Rapid Diagnosis of HIV-1 virus by Near Infrared Spectroscopy: based on Partial least squares regression |
| title_full_unstemmed | Rapid Diagnosis of HIV-1 virus by Near Infrared Spectroscopy: based on Partial least squares regression |
| title_short | Rapid Diagnosis of HIV-1 virus by Near Infrared Spectroscopy: based on Partial least squares regression |
| title_sort | rapid diagnosis of hiv 1 virus by near infrared spectroscopy based on partial least squares regression |
| url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2021/47/e3sconf_icepe2021_03067.pdf |
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