A hydrogel biosensor for high selective and sensitive detection of amyloid-beta oligomers
Liping Sun,1 Yong Zhong,1 Jie Gui,1 Xianwu Wang,1 Xiaorong Zhuang,2 Jian Weng1 1Key Laboratory of Biomedical Engineering of Fujian Province, Research Center of Biomedical Engineering of Xiamen, Department of Biomaterials, College of Materials, Xiamen University, 2Department of Neurology, The Affili...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2018-02-01
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| Series: | International Journal of Nanomedicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/a-hydrogel-biosensor-for-high-selective-and-sensitive-detection-of-amy-peer-reviewed-article-IJN |
| _version_ | 1818979866965442560 |
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| author | Sun LP Zhong Y Gui J Wang XW Zhuang XR Weng J |
| author_facet | Sun LP Zhong Y Gui J Wang XW Zhuang XR Weng J |
| author_sort | Sun LP |
| collection | DOAJ |
| description | Liping Sun,1 Yong Zhong,1 Jie Gui,1 Xianwu Wang,1 Xiaorong Zhuang,2 Jian Weng1 1Key Laboratory of Biomedical Engineering of Fujian Province, Research Center of Biomedical Engineering of Xiamen, Department of Biomaterials, College of Materials, Xiamen University, 2Department of Neurology, The Affiliated Zhongshan Hospital of Xiamen University, Xiamen, People’s Republic of China Background: Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by progressive cognitive and memory impairment. It is the most common neurological disease that causes dementia. Soluble amyloid-beta oligomers (AβO) in blood or cerebrospinal fluid (CSF) are the pathogenic biomarker correlated with AD. Methods: A simple electrochemical biosensor using graphene oxide/gold nanoparticles (GNPs) hydrogel electrode was developed in this study. Thiolated cellular prion protein (PrPC) peptide probe was immobilized on GNPs of the hydrogel electrode to construct an AβO biosensor. Electrochemical impedance spectroscopy was utilized for AβO analysis. Results: The specific binding between AβO and PrPC probes on the hydrogel electrode resulted in an increase in the electron-transfer resistance. The biosensor showed high specificity and sensitivity for AβO detection. It could selectively differentiate AβO from amyloid-beta (Aβ) monomers or fibrils. Meanwhile, it was highly sensitive to detect as low as 0.1 pM AβO in artificial CSF or blood plasma. The linear range for AβO detection is from 0.1 pM to 10 nM. Conclusion: This biosensor could be used as a cost-effective tool for early diagnosis of AD due to its high electrochemical performance and bionic structure. Keywords: Alzheimer’s disease, amyloid-beta oligomer, graphene, gold nanoparticles, biosensor |
| first_indexed | 2024-12-20T17:06:21Z |
| format | Article |
| id | doaj.art-309b8aaac94b42878e9c3cd814eab571 |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-20T17:06:21Z |
| publishDate | 2018-02-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-309b8aaac94b42878e9c3cd814eab5712022-12-21T19:32:16ZengDove Medical PressInternational Journal of Nanomedicine1178-20132018-02-01Volume 1384385636722A hydrogel biosensor for high selective and sensitive detection of amyloid-beta oligomersSun LPZhong YGui JWang XWZhuang XRWeng JLiping Sun,1 Yong Zhong,1 Jie Gui,1 Xianwu Wang,1 Xiaorong Zhuang,2 Jian Weng1 1Key Laboratory of Biomedical Engineering of Fujian Province, Research Center of Biomedical Engineering of Xiamen, Department of Biomaterials, College of Materials, Xiamen University, 2Department of Neurology, The Affiliated Zhongshan Hospital of Xiamen University, Xiamen, People’s Republic of China Background: Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by progressive cognitive and memory impairment. It is the most common neurological disease that causes dementia. Soluble amyloid-beta oligomers (AβO) in blood or cerebrospinal fluid (CSF) are the pathogenic biomarker correlated with AD. Methods: A simple electrochemical biosensor using graphene oxide/gold nanoparticles (GNPs) hydrogel electrode was developed in this study. Thiolated cellular prion protein (PrPC) peptide probe was immobilized on GNPs of the hydrogel electrode to construct an AβO biosensor. Electrochemical impedance spectroscopy was utilized for AβO analysis. Results: The specific binding between AβO and PrPC probes on the hydrogel electrode resulted in an increase in the electron-transfer resistance. The biosensor showed high specificity and sensitivity for AβO detection. It could selectively differentiate AβO from amyloid-beta (Aβ) monomers or fibrils. Meanwhile, it was highly sensitive to detect as low as 0.1 pM AβO in artificial CSF or blood plasma. The linear range for AβO detection is from 0.1 pM to 10 nM. Conclusion: This biosensor could be used as a cost-effective tool for early diagnosis of AD due to its high electrochemical performance and bionic structure. Keywords: Alzheimer’s disease, amyloid-beta oligomer, graphene, gold nanoparticles, biosensorhttps://www.dovepress.com/a-hydrogel-biosensor-for-high-selective-and-sensitive-detection-of-amy-peer-reviewed-article-IJNAlzheimer’s diseaseamyloid-beta oligomergraphenegold nanoparticlesbiosensor |
| spellingShingle | Sun LP Zhong Y Gui J Wang XW Zhuang XR Weng J A hydrogel biosensor for high selective and sensitive detection of amyloid-beta oligomers International Journal of Nanomedicine Alzheimer’s disease amyloid-beta oligomer graphene gold nanoparticles biosensor |
| title | A hydrogel biosensor for high selective and sensitive detection of amyloid-beta oligomers |
| title_full | A hydrogel biosensor for high selective and sensitive detection of amyloid-beta oligomers |
| title_fullStr | A hydrogel biosensor for high selective and sensitive detection of amyloid-beta oligomers |
| title_full_unstemmed | A hydrogel biosensor for high selective and sensitive detection of amyloid-beta oligomers |
| title_short | A hydrogel biosensor for high selective and sensitive detection of amyloid-beta oligomers |
| title_sort | hydrogel biosensor for high selective and sensitive detection of amyloid beta oligomers |
| topic | Alzheimer’s disease amyloid-beta oligomer graphene gold nanoparticles biosensor |
| url | https://www.dovepress.com/a-hydrogel-biosensor-for-high-selective-and-sensitive-detection-of-amy-peer-reviewed-article-IJN |
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