Antimicrobial Resistance Determinants Circulating among Thermophilic <i>Campylobacter</i> Isolates Recovered from Broilers in Ireland Over a One-Year Period
Campylobacteriosis is the leading cause of human bacterial gastroenteritis, very often associated with poultry consumption. Thermophilic <i>Campylobacter</i> (<i>Campylobacter jejuni</i> and <i>Campylobacter coli</i>) isolates (<i>n</i> = 158) recovere...
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| Format: | Article |
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MDPI AG
2020-06-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/9/6/308 |
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| author | Caoimhe T. Lynch Helen Lynch Sarah Burke Kayleigh Hawkins Colin Buttimer Conor Mc Carthy John Egan Paul Whyte Declan Bolton Aidan Coffey Brigid Lucey |
| author_facet | Caoimhe T. Lynch Helen Lynch Sarah Burke Kayleigh Hawkins Colin Buttimer Conor Mc Carthy John Egan Paul Whyte Declan Bolton Aidan Coffey Brigid Lucey |
| author_sort | Caoimhe T. Lynch |
| collection | DOAJ |
| description | Campylobacteriosis is the leading cause of human bacterial gastroenteritis, very often associated with poultry consumption. Thermophilic <i>Campylobacter</i> (<i>Campylobacter jejuni</i> and <i>Campylobacter coli</i>) isolates (<i>n</i> = 158) recovered from broiler neck skin and caecal contents in Ireland over a one-year period, resistant to at least one of three clinically relevant antimicrobial classes, were screened for resistance determinants. All ciprofloxacin-resistant isolates (<i>n</i> = 99) harboured the C257T nucleotide mutation (conferring the Thr-86-Ile substitution) in conjunction with other synonymous and nonsynonymous mutations, which may have epidemiological value. The A2075G nucleotide mutation and amino acid substitutions in L4 and L22 were detected in all erythromycin-resistant isolates (<i>n</i> = 5). The <i>tetO</i> gene was detected in 100% (<i>n</i> = 119) of tetracycline-resistant isolates and three of which were found to harbour the mosaic tetracycline resistance gene <i>tetO/32/O</i>. Two streptomycin-resistant <i>C. jejuni</i> isolates (isolated from the same flock) harboured <i>ant(6)-Ib</i>, located in a multidrug resistance genomic island, containing aminoglycoside, streptothricin (<i>satA</i>) and tetracycline resistance genes (truncated <i>tetO</i> and mosaic <i>tetO/32/O</i>). The <i>ant(6)-Ie</i> gene was identified in two streptomycin-resistant <i>C. coli</i> isolates. This study highlights the widespread acquisition of antimicrobial resistance determinants among chicken-associated <i>Campylobacter</i> isolates, through horizontal gene transfer or clonal expansion of resistant lineages. The stability of such resistance determinants is compounded by the fluidity of mobile genetic element. |
| first_indexed | 2024-03-10T19:17:44Z |
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| id | doaj.art-309fb5a117ae4a12bff4e37a85cb7377 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T19:17:44Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-309fb5a117ae4a12bff4e37a85cb73772023-11-20T03:11:49ZengMDPI AGAntibiotics2079-63822020-06-019630810.3390/antibiotics9060308Antimicrobial Resistance Determinants Circulating among Thermophilic <i>Campylobacter</i> Isolates Recovered from Broilers in Ireland Over a One-Year PeriodCaoimhe T. Lynch0Helen Lynch1Sarah Burke2Kayleigh Hawkins3Colin Buttimer4Conor Mc Carthy5John Egan6Paul Whyte7Declan Bolton8Aidan Coffey9Brigid Lucey10Department of Biological Sciences, Cork Institute of Technology, Bishopstown, T12 P928 Cork, IrelandNRL Campylobacter, Backweston Laboratory Complex, Young’s Cross, Celbridge, W23 X3PH Kildare, IrelandDepartment of Biological Sciences, Cork Institute of Technology, Bishopstown, T12 P928 Cork, IrelandDepartment of Biological Sciences, Cork Institute of Technology, Bishopstown, T12 P928 Cork, IrelandAPC Microbiome Ireland, University College Cork, T12 YT20 Cork, IrelandDepartment of Biological Sciences, Cork Institute of Technology, Bishopstown, T12 P928 Cork, IrelandNRL Campylobacter, Backweston Laboratory Complex, Young’s Cross, Celbridge, W23 X3PH Kildare, IrelandSchool of Veterinary Medicine, University College Dublin, Belfield, D04 V1W8 Dublin 4, IrelandTeagasc Food Research Centre, Ashtown, D15 DY05 Dublin 15, IrelandDepartment of Biological Sciences, Cork Institute of Technology, Bishopstown, T12 P928 Cork, IrelandDepartment of Biological Sciences, Cork Institute of Technology, Bishopstown, T12 P928 Cork, IrelandCampylobacteriosis is the leading cause of human bacterial gastroenteritis, very often associated with poultry consumption. Thermophilic <i>Campylobacter</i> (<i>Campylobacter jejuni</i> and <i>Campylobacter coli</i>) isolates (<i>n</i> = 158) recovered from broiler neck skin and caecal contents in Ireland over a one-year period, resistant to at least one of three clinically relevant antimicrobial classes, were screened for resistance determinants. All ciprofloxacin-resistant isolates (<i>n</i> = 99) harboured the C257T nucleotide mutation (conferring the Thr-86-Ile substitution) in conjunction with other synonymous and nonsynonymous mutations, which may have epidemiological value. The A2075G nucleotide mutation and amino acid substitutions in L4 and L22 were detected in all erythromycin-resistant isolates (<i>n</i> = 5). The <i>tetO</i> gene was detected in 100% (<i>n</i> = 119) of tetracycline-resistant isolates and three of which were found to harbour the mosaic tetracycline resistance gene <i>tetO/32/O</i>. Two streptomycin-resistant <i>C. jejuni</i> isolates (isolated from the same flock) harboured <i>ant(6)-Ib</i>, located in a multidrug resistance genomic island, containing aminoglycoside, streptothricin (<i>satA</i>) and tetracycline resistance genes (truncated <i>tetO</i> and mosaic <i>tetO/32/O</i>). The <i>ant(6)-Ie</i> gene was identified in two streptomycin-resistant <i>C. coli</i> isolates. This study highlights the widespread acquisition of antimicrobial resistance determinants among chicken-associated <i>Campylobacter</i> isolates, through horizontal gene transfer or clonal expansion of resistant lineages. The stability of such resistance determinants is compounded by the fluidity of mobile genetic element.https://www.mdpi.com/2079-6382/9/6/308<i>Campylobacter</i>antimicrobial resistanceciprofloxacintetracyclineaminoglycosidesmacrolides |
| spellingShingle | Caoimhe T. Lynch Helen Lynch Sarah Burke Kayleigh Hawkins Colin Buttimer Conor Mc Carthy John Egan Paul Whyte Declan Bolton Aidan Coffey Brigid Lucey Antimicrobial Resistance Determinants Circulating among Thermophilic <i>Campylobacter</i> Isolates Recovered from Broilers in Ireland Over a One-Year Period Antibiotics <i>Campylobacter</i> antimicrobial resistance ciprofloxacin tetracycline aminoglycosides macrolides |
| title | Antimicrobial Resistance Determinants Circulating among Thermophilic <i>Campylobacter</i> Isolates Recovered from Broilers in Ireland Over a One-Year Period |
| title_full | Antimicrobial Resistance Determinants Circulating among Thermophilic <i>Campylobacter</i> Isolates Recovered from Broilers in Ireland Over a One-Year Period |
| title_fullStr | Antimicrobial Resistance Determinants Circulating among Thermophilic <i>Campylobacter</i> Isolates Recovered from Broilers in Ireland Over a One-Year Period |
| title_full_unstemmed | Antimicrobial Resistance Determinants Circulating among Thermophilic <i>Campylobacter</i> Isolates Recovered from Broilers in Ireland Over a One-Year Period |
| title_short | Antimicrobial Resistance Determinants Circulating among Thermophilic <i>Campylobacter</i> Isolates Recovered from Broilers in Ireland Over a One-Year Period |
| title_sort | antimicrobial resistance determinants circulating among thermophilic i campylobacter i isolates recovered from broilers in ireland over a one year period |
| topic | <i>Campylobacter</i> antimicrobial resistance ciprofloxacin tetracycline aminoglycosides macrolides |
| url | https://www.mdpi.com/2079-6382/9/6/308 |
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