Protective Effect of Shikimic Acid against Cisplatin-Induced Renal Injury: In Vitro and In Vivo Studies
Nephrotoxicity is a serious side effect of cisplatin, which is one of the most frequently used drugs for cancer treatment. This study aimed to assess the renoprotective effect of <i>Artemisia absinthium</i> extract and its bioactive compound (shikimic acid) against cisplatin-induced rena...
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MDPI AG
2020-12-01
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| Online Access: | https://www.mdpi.com/2223-7747/9/12/1681 |
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| author | Jinkyung Lee Quynh Nhu Nguyen Jun Yeon Park Sullim Lee Gwi Seo Hwang Noriko Yamabe Sungyoul Choi Ki Sung Kang |
| author_facet | Jinkyung Lee Quynh Nhu Nguyen Jun Yeon Park Sullim Lee Gwi Seo Hwang Noriko Yamabe Sungyoul Choi Ki Sung Kang |
| author_sort | Jinkyung Lee |
| collection | DOAJ |
| description | Nephrotoxicity is a serious side effect of cisplatin, which is one of the most frequently used drugs for cancer treatment. This study aimed to assess the renoprotective effect of <i>Artemisia absinthium</i> extract and its bioactive compound (shikimic acid) against cisplatin-induced renal injury. An in vitro assay was performed in kidney tubular epithelial cells (LLC-PK1) with 50, 100, and 200 µg/mL <i>A. absinthium</i> extract and 25 and 50 µM shikimic acid, and cytotoxicity was induced by 25 µM cisplatin. BALB/c mice (6 weeks old) were injected with 16 mg/kg cisplatin once and orally administered 25 and 50 mg/kg shikimic acid daily for 4 days. The results showed that the <i>A. absinthium</i> extract reversed the decrease in renal cell viability induced by cisplatin, whereas it decreased the reactive oxidative stress accumulation and apoptosis in LLC-PK1 cells. Shikimic acid also reversed the effect on cell viability but decreased oxidative stress and apoptosis in renal cells compared with the levels in the cisplatin-treated group. Furthermore, shikimic acid protected against kidney injury in cisplatin-treated mice by reducing serum creatinine levels. The protective effect of shikimic acid against cisplatin-mediated kidney injury was confirmed by the recovery of histological kidney injury in cisplatin-treated mice. To the best of our knowledge, this study is the first report on the nephroprotective effect of <i>A. absinthium</i> extract and its mechanism of action against cisplatin-induced renal injury. |
| first_indexed | 2024-03-10T14:25:02Z |
| format | Article |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-10T14:25:02Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj.art-30a0dd9d285f48afa9b17ac45794b2902023-11-20T23:04:16ZengMDPI AGPlants2223-77472020-12-01912168110.3390/plants9121681Protective Effect of Shikimic Acid against Cisplatin-Induced Renal Injury: In Vitro and In Vivo StudiesJinkyung Lee0Quynh Nhu Nguyen1Jun Yeon Park2Sullim Lee3Gwi Seo Hwang4Noriko Yamabe5Sungyoul Choi6Ki Sung Kang7College of Korean Medicine, Gachon University, Seongnam 13120, KoreaCollege of Korean Medicine, Gachon University, Seongnam 13120, KoreaDepartment of Food Science and Biotechnology, Kyonggi University, Suwon 16227, KoreaCollege of Bio-Nano Technology, Gachon University, Seongnam-si, Gyeonggi-do 13120, KoreaCollege of Korean Medicine, Gachon University, Seongnam 13120, KoreaCollege of Korean Medicine, Gachon University, Seongnam 13120, KoreaCollege of Korean Medicine, Gachon University, Seongnam 13120, KoreaCollege of Korean Medicine, Gachon University, Seongnam 13120, KoreaNephrotoxicity is a serious side effect of cisplatin, which is one of the most frequently used drugs for cancer treatment. This study aimed to assess the renoprotective effect of <i>Artemisia absinthium</i> extract and its bioactive compound (shikimic acid) against cisplatin-induced renal injury. An in vitro assay was performed in kidney tubular epithelial cells (LLC-PK1) with 50, 100, and 200 µg/mL <i>A. absinthium</i> extract and 25 and 50 µM shikimic acid, and cytotoxicity was induced by 25 µM cisplatin. BALB/c mice (6 weeks old) were injected with 16 mg/kg cisplatin once and orally administered 25 and 50 mg/kg shikimic acid daily for 4 days. The results showed that the <i>A. absinthium</i> extract reversed the decrease in renal cell viability induced by cisplatin, whereas it decreased the reactive oxidative stress accumulation and apoptosis in LLC-PK1 cells. Shikimic acid also reversed the effect on cell viability but decreased oxidative stress and apoptosis in renal cells compared with the levels in the cisplatin-treated group. Furthermore, shikimic acid protected against kidney injury in cisplatin-treated mice by reducing serum creatinine levels. The protective effect of shikimic acid against cisplatin-mediated kidney injury was confirmed by the recovery of histological kidney injury in cisplatin-treated mice. To the best of our knowledge, this study is the first report on the nephroprotective effect of <i>A. absinthium</i> extract and its mechanism of action against cisplatin-induced renal injury.https://www.mdpi.com/2223-7747/9/12/1681<i>Artemisia absinthium</i>shikimic acidcisplatinnephrotoxicityapoptosis |
| spellingShingle | Jinkyung Lee Quynh Nhu Nguyen Jun Yeon Park Sullim Lee Gwi Seo Hwang Noriko Yamabe Sungyoul Choi Ki Sung Kang Protective Effect of Shikimic Acid against Cisplatin-Induced Renal Injury: In Vitro and In Vivo Studies Plants <i>Artemisia absinthium</i> shikimic acid cisplatin nephrotoxicity apoptosis |
| title | Protective Effect of Shikimic Acid against Cisplatin-Induced Renal Injury: In Vitro and In Vivo Studies |
| title_full | Protective Effect of Shikimic Acid against Cisplatin-Induced Renal Injury: In Vitro and In Vivo Studies |
| title_fullStr | Protective Effect of Shikimic Acid against Cisplatin-Induced Renal Injury: In Vitro and In Vivo Studies |
| title_full_unstemmed | Protective Effect of Shikimic Acid against Cisplatin-Induced Renal Injury: In Vitro and In Vivo Studies |
| title_short | Protective Effect of Shikimic Acid against Cisplatin-Induced Renal Injury: In Vitro and In Vivo Studies |
| title_sort | protective effect of shikimic acid against cisplatin induced renal injury in vitro and in vivo studies |
| topic | <i>Artemisia absinthium</i> shikimic acid cisplatin nephrotoxicity apoptosis |
| url | https://www.mdpi.com/2223-7747/9/12/1681 |
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