Characterization of a miRNA Signature with Enhanced Diagnostic and Prognostic Power for Patients with Bladder Carcinoma
Bladder carcinoma is globally among the most prevalent cancers and is associated with a high mortality rate at advanced stages. Its detection relies on invasive diagnostic methods that are unpleasant for the patient. Non-invasive molecular biomarkers, such as miRNAs, could serve as alternatives for...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/22/16243 |
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| author | Maria Samara Panagiotis J. Vlachostergios Eleni Thodou Ioannis Zachos Lampros Mitrakas Konstantinos Evmorfopoulos Vassilios Tzortzis Antonis Giakountis |
| author_facet | Maria Samara Panagiotis J. Vlachostergios Eleni Thodou Ioannis Zachos Lampros Mitrakas Konstantinos Evmorfopoulos Vassilios Tzortzis Antonis Giakountis |
| author_sort | Maria Samara |
| collection | DOAJ |
| description | Bladder carcinoma is globally among the most prevalent cancers and is associated with a high mortality rate at advanced stages. Its detection relies on invasive diagnostic methods that are unpleasant for the patient. Non-invasive molecular biomarkers, such as miRNAs, could serve as alternatives for early detection and prognosis of this malignancy. We designed a computational approach that combines transcriptome profiling, survival analyses, and calculation of diagnostic power in order to isolate miRNA signatures with high diagnostic and prognostic utility. Our analysis of TCGA-BLCA data from 429 patients yielded one miRNA signature, consisting of five upregulated and three downregulated miRNAs with cumulative diagnostic power that outperforms current diagnostic methods. The same miRNAs have a strong prognostic significance since their expression is associated with the overall survival of bladder cancer patients. We evaluated the expression of this signature in 19 solid cancer types, supporting its unique diagnostic utility for bladder carcinoma. We provide computational evidence regarding the functional implications of this miRNA signature in cell cycle regulation, demonstrating its abundance in body fluids, including peripheral blood and urine. Our study characterized a novel miRNA signature with the potential to serve as a non-invasive method for bladder cancer diagnosis and prognosis. |
| first_indexed | 2024-03-09T16:45:43Z |
| format | Article |
| id | doaj.art-30a241a1740147e8aa4505e93846698a |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T16:45:43Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-30a241a1740147e8aa4505e93846698a2023-11-24T14:46:43ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-11-0124221624310.3390/ijms242216243Characterization of a miRNA Signature with Enhanced Diagnostic and Prognostic Power for Patients with Bladder CarcinomaMaria Samara0Panagiotis J. Vlachostergios1Eleni Thodou2Ioannis Zachos3Lampros Mitrakas4Konstantinos Evmorfopoulos5Vassilios Tzortzis6Antonis Giakountis7Department of Pathology, Faculty of Medicine, School of Health Sciences, University of Thessaly, 41335 Larissa, GreeceDepartment of Medicine, Division of Hematology and Medical Oncology, Weill Cornell Medicine, New York, NY 10065, USADepartment of Pathology, Faculty of Medicine, School of Health Sciences, University of Thessaly, 41335 Larissa, GreeceDepartment of Urology, Faculty of Medicine, School of Health Sciences, University of Thessaly, University Hospital of Larissa, 41335 Larissa, GreeceDepartment of Urology, Faculty of Medicine, School of Health Sciences, University of Thessaly, University Hospital of Larissa, 41335 Larissa, GreeceDepartment of Urology, Faculty of Medicine, School of Health Sciences, University of Thessaly, University Hospital of Larissa, 41335 Larissa, GreeceDepartment of Urology, Faculty of Medicine, School of Health Sciences, University of Thessaly, University Hospital of Larissa, 41335 Larissa, GreeceDepartment of Biochemistry and Biotechnology, University of Thessaly, 41335 Larissa, GreeceBladder carcinoma is globally among the most prevalent cancers and is associated with a high mortality rate at advanced stages. Its detection relies on invasive diagnostic methods that are unpleasant for the patient. Non-invasive molecular biomarkers, such as miRNAs, could serve as alternatives for early detection and prognosis of this malignancy. We designed a computational approach that combines transcriptome profiling, survival analyses, and calculation of diagnostic power in order to isolate miRNA signatures with high diagnostic and prognostic utility. Our analysis of TCGA-BLCA data from 429 patients yielded one miRNA signature, consisting of five upregulated and three downregulated miRNAs with cumulative diagnostic power that outperforms current diagnostic methods. The same miRNAs have a strong prognostic significance since their expression is associated with the overall survival of bladder cancer patients. We evaluated the expression of this signature in 19 solid cancer types, supporting its unique diagnostic utility for bladder carcinoma. We provide computational evidence regarding the functional implications of this miRNA signature in cell cycle regulation, demonstrating its abundance in body fluids, including peripheral blood and urine. Our study characterized a novel miRNA signature with the potential to serve as a non-invasive method for bladder cancer diagnosis and prognosis.https://www.mdpi.com/1422-0067/24/22/16243miRNAsbladder carcinomadiagnosis and prognosisgenitourinary transcriptomicsROC analysis |
| spellingShingle | Maria Samara Panagiotis J. Vlachostergios Eleni Thodou Ioannis Zachos Lampros Mitrakas Konstantinos Evmorfopoulos Vassilios Tzortzis Antonis Giakountis Characterization of a miRNA Signature with Enhanced Diagnostic and Prognostic Power for Patients with Bladder Carcinoma International Journal of Molecular Sciences miRNAs bladder carcinoma diagnosis and prognosis genitourinary transcriptomics ROC analysis |
| title | Characterization of a miRNA Signature with Enhanced Diagnostic and Prognostic Power for Patients with Bladder Carcinoma |
| title_full | Characterization of a miRNA Signature with Enhanced Diagnostic and Prognostic Power for Patients with Bladder Carcinoma |
| title_fullStr | Characterization of a miRNA Signature with Enhanced Diagnostic and Prognostic Power for Patients with Bladder Carcinoma |
| title_full_unstemmed | Characterization of a miRNA Signature with Enhanced Diagnostic and Prognostic Power for Patients with Bladder Carcinoma |
| title_short | Characterization of a miRNA Signature with Enhanced Diagnostic and Prognostic Power for Patients with Bladder Carcinoma |
| title_sort | characterization of a mirna signature with enhanced diagnostic and prognostic power for patients with bladder carcinoma |
| topic | miRNAs bladder carcinoma diagnosis and prognosis genitourinary transcriptomics ROC analysis |
| url | https://www.mdpi.com/1422-0067/24/22/16243 |
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