Incorporation of FGF-2 into Pharmaceutical Grade Fucoidan/Chitosan Polyelectrolyte Multilayers
Biopolymer polyelectrolyte multilayers are a commonly studied soft matter system for wound healing applications due to the biocompatibility and beneficial properties of naturally occurring polyelectrolytes. In this work, a popular biopolymer, chitosan, was combined with the lesser known polysacchari...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/18/11/531 |
| _version_ | 1797549801785524224 |
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| author | Natalie L. Benbow Samuel Karpiniec Marta Krasowska David A. Beattie |
| author_facet | Natalie L. Benbow Samuel Karpiniec Marta Krasowska David A. Beattie |
| author_sort | Natalie L. Benbow |
| collection | DOAJ |
| description | Biopolymer polyelectrolyte multilayers are a commonly studied soft matter system for wound healing applications due to the biocompatibility and beneficial properties of naturally occurring polyelectrolytes. In this work, a popular biopolymer, chitosan, was combined with the lesser known polysaccharide, fucoidan, to create a multilayer film capable of sequestering growth factor for later release. Fucoidan has been shown to act as a heparin-mimic due to similarities in the structure of the two molecules, however, the binding of fibroblast growth factor-2 to fucoidan has not been demonstrated in a multilayer system. This study assesses the ability of fucoidan to bind fibroblast growth factor-2 within a fucoidan/chitosan polyelectrolyte multilayer structure using attenuated total internal reflectance infrared spectroscopy and quartz crystal microbalance with dissipation monitoring. The fibroblast growth factor-2 was sequestered into the polyelectrolyte multilayer as a cationic layer in the uppermost layers of the film structure. In addition, the diffusion of fibroblast growth factor-2 into the multilayer has been assessed. |
| first_indexed | 2024-03-10T15:19:33Z |
| format | Article |
| id | doaj.art-30a75d7924f7477abe4796d0ff38cc23 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T15:19:33Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-30a75d7924f7477abe4796d0ff38cc232023-11-20T18:35:18ZengMDPI AGMarine Drugs1660-33972020-10-01181153110.3390/md18110531Incorporation of FGF-2 into Pharmaceutical Grade Fucoidan/Chitosan Polyelectrolyte MultilayersNatalie L. Benbow0Samuel Karpiniec1Marta Krasowska2David A. Beattie3Future Industries Institute, University of South Australia, Mawson Lakes, SA 5095, AustraliaMarinova, Cambridge, TAS 7170, AustraliaFuture Industries Institute, University of South Australia, Mawson Lakes, SA 5095, AustraliaFuture Industries Institute, University of South Australia, Mawson Lakes, SA 5095, AustraliaBiopolymer polyelectrolyte multilayers are a commonly studied soft matter system for wound healing applications due to the biocompatibility and beneficial properties of naturally occurring polyelectrolytes. In this work, a popular biopolymer, chitosan, was combined with the lesser known polysaccharide, fucoidan, to create a multilayer film capable of sequestering growth factor for later release. Fucoidan has been shown to act as a heparin-mimic due to similarities in the structure of the two molecules, however, the binding of fibroblast growth factor-2 to fucoidan has not been demonstrated in a multilayer system. This study assesses the ability of fucoidan to bind fibroblast growth factor-2 within a fucoidan/chitosan polyelectrolyte multilayer structure using attenuated total internal reflectance infrared spectroscopy and quartz crystal microbalance with dissipation monitoring. The fibroblast growth factor-2 was sequestered into the polyelectrolyte multilayer as a cationic layer in the uppermost layers of the film structure. In addition, the diffusion of fibroblast growth factor-2 into the multilayer has been assessed.https://www.mdpi.com/1660-3397/18/11/531fucoidanchitosanfibroblast growth factor-2polyelectrolyte multilayerinfrared spectroscopyquartz crystal microbalance |
| spellingShingle | Natalie L. Benbow Samuel Karpiniec Marta Krasowska David A. Beattie Incorporation of FGF-2 into Pharmaceutical Grade Fucoidan/Chitosan Polyelectrolyte Multilayers Marine Drugs fucoidan chitosan fibroblast growth factor-2 polyelectrolyte multilayer infrared spectroscopy quartz crystal microbalance |
| title | Incorporation of FGF-2 into Pharmaceutical Grade Fucoidan/Chitosan Polyelectrolyte Multilayers |
| title_full | Incorporation of FGF-2 into Pharmaceutical Grade Fucoidan/Chitosan Polyelectrolyte Multilayers |
| title_fullStr | Incorporation of FGF-2 into Pharmaceutical Grade Fucoidan/Chitosan Polyelectrolyte Multilayers |
| title_full_unstemmed | Incorporation of FGF-2 into Pharmaceutical Grade Fucoidan/Chitosan Polyelectrolyte Multilayers |
| title_short | Incorporation of FGF-2 into Pharmaceutical Grade Fucoidan/Chitosan Polyelectrolyte Multilayers |
| title_sort | incorporation of fgf 2 into pharmaceutical grade fucoidan chitosan polyelectrolyte multilayers |
| topic | fucoidan chitosan fibroblast growth factor-2 polyelectrolyte multilayer infrared spectroscopy quartz crystal microbalance |
| url | https://www.mdpi.com/1660-3397/18/11/531 |
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